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Proteolytic gut activities in the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae).
Arch Insect Biochem Physiol. 2003 May; 53(1):19-29.AI

Abstract

Digestive endoprotease activities of the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae), were characterized based on the ability of gut extracts to hydrolyze specific synthetic substrates, optimal pH, and hydrolysis sensitivity to protease inhibitors. Larvae of this species were found to use a complex proteolytic system that includes cathepsin D-, cathepsin B-, trypsin-, and chymotrypsin-like activities. Trypsin-like activity was evenly distributed among the anterior, middle, and posterior portions of the gut, whereas cathepsin B- and cathepsin D-like activities were mainly located in the anterior and middle sections, and the chymotrypsin-like activity was highest in the middle and posterior sections. Gelatin-containing native-PAGE gels indicated the presence of several aspartyl, cysteine, and serine protease forms and confirmed the spatial organization of the proteolytic digestive process.

Authors+Show Affiliations

Center for Genetic Engineering and Biotechnology, Sancti Spiritus, Cuba.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12701111

Citation

Hernández, Carlos A., et al. "Proteolytic Gut Activities in the Rice Water Weevil, Lissorhoptrus Brevirostris Suffrian (Coleoptera: Curculionidae)." Archives of Insect Biochemistry and Physiology, vol. 53, no. 1, 2003, pp. 19-29.
Hernández CA, Pujol M, Alfonso-Rubí J, et al. Proteolytic gut activities in the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae). Arch Insect Biochem Physiol. 2003;53(1):19-29.
Hernández, C. A., Pujol, M., Alfonso-Rubí, J., Armas, R., Coll, Y., Pérez, M., González, A., Ruiz, M., Castañera, P., & Ortego, F. (2003). Proteolytic gut activities in the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae). Archives of Insect Biochemistry and Physiology, 53(1), 19-29.
Hernández CA, et al. Proteolytic Gut Activities in the Rice Water Weevil, Lissorhoptrus Brevirostris Suffrian (Coleoptera: Curculionidae). Arch Insect Biochem Physiol. 2003;53(1):19-29. PubMed PMID: 12701111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteolytic gut activities in the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae). AU - Hernández,Carlos A, AU - Pujol,Merardo, AU - Alfonso-Rubí,Julio, AU - Armas,Raúl, AU - Coll,Yamilet, AU - Pérez,Maylin, AU - González,Annerys, AU - Ruiz,Marisa, AU - Castañera,Pedro, AU - Ortego,Félix, PY - 2003/4/18/pubmed PY - 2003/6/5/medline PY - 2003/4/18/entrez SP - 19 EP - 29 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 53 IS - 1 N2 - Digestive endoprotease activities of the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae), were characterized based on the ability of gut extracts to hydrolyze specific synthetic substrates, optimal pH, and hydrolysis sensitivity to protease inhibitors. Larvae of this species were found to use a complex proteolytic system that includes cathepsin D-, cathepsin B-, trypsin-, and chymotrypsin-like activities. Trypsin-like activity was evenly distributed among the anterior, middle, and posterior portions of the gut, whereas cathepsin B- and cathepsin D-like activities were mainly located in the anterior and middle sections, and the chymotrypsin-like activity was highest in the middle and posterior sections. Gelatin-containing native-PAGE gels indicated the presence of several aspartyl, cysteine, and serine protease forms and confirmed the spatial organization of the proteolytic digestive process. SN - 0739-4462 UR - https://www.unboundmedicine.com/medline/citation/12701111/Proteolytic_gut_activities_in_the_rice_water_weevil_Lissorhoptrus_brevirostris_Suffrian__Coleoptera:_Curculionidae__ L2 - https://doi.org/10.1002/arch.10083 DB - PRIME DP - Unbound Medicine ER -