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Amylin in human pancreatic islets.
Pathology. 2003 Feb; 35(1):34-6.P

Abstract

AIMS

Double and triple immunocytochemical staining was used to co-localise amylin with four pancreatic hormones, including insulin, glucagon, somatostatin and pancreatic polypeptide.

METHODS

Normal human adult islets were initially stained for amylin using the avidin-biotin alkaline phosphatase method. The stained sections were subsequently stained for four pancreatic hormones, respectively, with the immunoperoxidase method.

RESULTS

The same islet cells that were positively stained for amylin, were also positively stained for insulin, and were positively stained for glucagon, somatostatin and pancreatic polypeptide in lesser cell numbers.

CONCLUSIONS

Amylin is known to co-secrete and is co-localised with insulin and three other islet hormones are co-localised with amylin. This co-localisation of amylin with all four pancreatic hormones may support a role for amylin in secretory processes of these hormones.

Authors+Show Affiliations

Department of Pathology, University of Kansas Medical Center, Kansas City, Kansas, USA. Ttomita@ttmcelp.ttuhsc.edu

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12701681

Citation

Tomita, T. "Amylin in Human Pancreatic Islets." Pathology, vol. 35, no. 1, 2003, pp. 34-6.
Tomita T. Amylin in human pancreatic islets. Pathology. 2003;35(1):34-6.
Tomita, T. (2003). Amylin in human pancreatic islets. Pathology, 35(1), 34-6.
Tomita T. Amylin in Human Pancreatic Islets. Pathology. 2003;35(1):34-6. PubMed PMID: 12701681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amylin in human pancreatic islets. A1 - Tomita,T, PY - 2003/4/19/pubmed PY - 2003/10/17/medline PY - 2003/4/19/entrez SP - 34 EP - 6 JF - Pathology JO - Pathology VL - 35 IS - 1 N2 - AIMS: Double and triple immunocytochemical staining was used to co-localise amylin with four pancreatic hormones, including insulin, glucagon, somatostatin and pancreatic polypeptide. METHODS: Normal human adult islets were initially stained for amylin using the avidin-biotin alkaline phosphatase method. The stained sections were subsequently stained for four pancreatic hormones, respectively, with the immunoperoxidase method. RESULTS: The same islet cells that were positively stained for amylin, were also positively stained for insulin, and were positively stained for glucagon, somatostatin and pancreatic polypeptide in lesser cell numbers. CONCLUSIONS: Amylin is known to co-secrete and is co-localised with insulin and three other islet hormones are co-localised with amylin. This co-localisation of amylin with all four pancreatic hormones may support a role for amylin in secretory processes of these hormones. SN - 0031-3025 UR - https://www.unboundmedicine.com/medline/citation/12701681/Amylin_in_human_pancreatic_islets_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12701681.ui DB - PRIME DP - Unbound Medicine ER -