Tags

Type your tag names separated by a space and hit enter

The diagnostic significance of enzyme linked immuno-sorbent assay for herpes simplex, varicella zoster and cytomegalovirus retinitis.
Indian J Ophthalmol. 2003 Mar; 51(1):71-5.IJ

Abstract

PURPOSE

To evaluate the diagnostic usefulness of enzyme linked immuno-sorbent assay (ELISA) in single serum samples to associate herpes simplex virus (HSV), varicella zoster virus (VZV) or cytomegalovirus (CMV) with viral retinitis as against polymerase chain reaction (PCR) on intraocular specimens. It was also designed to study the seroprevalence in normal healthy individuals, and the genomic prevalence of HSV, VZV and CMV in patients without an active viral inflammatory process.

METHODS

PCR for the detection of HSV, VZV and CMV genomes was done on 33 and 90 intraocular fluids from viral retinal patients and non-viral controls respectively. ELISA was done on 30 and 100 serum samples from viral retinitis patients and normal healthy controls respectively.

RESULTS

PCR did not detect HSV, VZV and CMV genomes except one, in which VZV-DNA was detected. ELISA showed prevalence rates of 28%, 83% and 90% for antibodies against HSV, VZV and CMV respectively in the normal population. In the 30 viral retinitis patients, PCR detected HSV-DNA in 2 (6.7%), VZV-DNA in 7 (23.3%) and CMV-DNA in 6 (20.0%) patients, while ELISA detected antibodies against HSV, VZV and CMV in 13 (43.3%), 24 (80.0%) and 23 (76.7%) patients respectively. ELISA was of value in indirect diagnosis only in 6 (20.0%) as compared to 15 (50.0%) of 30 patients by PCR, this difference was statistically significant (McNemar test, P value = 0.005).

CONCLUSION

Serology by ELISA is no longer a useful diagnostic tool to associate HSV, VZV and CMV viruses with viral retinitis.

Authors+Show Affiliations

L & T Microbiology Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, India. drhnm@sankaranethralaya.orgNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12701866

Citation

Madhavan, Hajib N., and Kannian Priya. "The Diagnostic Significance of Enzyme Linked Immuno-sorbent Assay for Herpes Simplex, Varicella Zoster and Cytomegalovirus Retinitis." Indian Journal of Ophthalmology, vol. 51, no. 1, 2003, pp. 71-5.
Madhavan HN, Priya K. The diagnostic significance of enzyme linked immuno-sorbent assay for herpes simplex, varicella zoster and cytomegalovirus retinitis. Indian J Ophthalmol. 2003;51(1):71-5.
Madhavan, H. N., & Priya, K. (2003). The diagnostic significance of enzyme linked immuno-sorbent assay for herpes simplex, varicella zoster and cytomegalovirus retinitis. Indian Journal of Ophthalmology, 51(1), 71-5.
Madhavan HN, Priya K. The Diagnostic Significance of Enzyme Linked Immuno-sorbent Assay for Herpes Simplex, Varicella Zoster and Cytomegalovirus Retinitis. Indian J Ophthalmol. 2003;51(1):71-5. PubMed PMID: 12701866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The diagnostic significance of enzyme linked immuno-sorbent assay for herpes simplex, varicella zoster and cytomegalovirus retinitis. AU - Madhavan,Hajib N, AU - Priya,Kannian, PY - 2003/4/19/pubmed PY - 2003/5/28/medline PY - 2003/4/19/entrez SP - 71 EP - 5 JF - Indian journal of ophthalmology JO - Indian J Ophthalmol VL - 51 IS - 1 N2 - PURPOSE: To evaluate the diagnostic usefulness of enzyme linked immuno-sorbent assay (ELISA) in single serum samples to associate herpes simplex virus (HSV), varicella zoster virus (VZV) or cytomegalovirus (CMV) with viral retinitis as against polymerase chain reaction (PCR) on intraocular specimens. It was also designed to study the seroprevalence in normal healthy individuals, and the genomic prevalence of HSV, VZV and CMV in patients without an active viral inflammatory process. METHODS: PCR for the detection of HSV, VZV and CMV genomes was done on 33 and 90 intraocular fluids from viral retinal patients and non-viral controls respectively. ELISA was done on 30 and 100 serum samples from viral retinitis patients and normal healthy controls respectively. RESULTS: PCR did not detect HSV, VZV and CMV genomes except one, in which VZV-DNA was detected. ELISA showed prevalence rates of 28%, 83% and 90% for antibodies against HSV, VZV and CMV respectively in the normal population. In the 30 viral retinitis patients, PCR detected HSV-DNA in 2 (6.7%), VZV-DNA in 7 (23.3%) and CMV-DNA in 6 (20.0%) patients, while ELISA detected antibodies against HSV, VZV and CMV in 13 (43.3%), 24 (80.0%) and 23 (76.7%) patients respectively. ELISA was of value in indirect diagnosis only in 6 (20.0%) as compared to 15 (50.0%) of 30 patients by PCR, this difference was statistically significant (McNemar test, P value = 0.005). CONCLUSION: Serology by ELISA is no longer a useful diagnostic tool to associate HSV, VZV and CMV viruses with viral retinitis. SN - 0301-4738 UR - https://www.unboundmedicine.com/medline/citation/12701866/The_diagnostic_significance_of_enzyme_linked_immuno_sorbent_assay_for_herpes_simplex_varicella_zoster_and_cytomegalovirus_retinitis_ L2 - http://www.ijo.in/article.asp?issn=0301-4738;year=2003;volume=51;issue=1;spage=71;epage=75;aulast=Madhavan DB - PRIME DP - Unbound Medicine ER -