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Characterisation of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters.
FEMS Yeast Res. 2002 Dec; 2(4):539-50.FY

Abstract

The yeast glucose transporters Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 and Gal2, individually expressed in an hxt1-7 null mutant strain, demonstrate the phenomenon of countertransport. Thus, these transporters, which are the most important glucose transporters in Saccharomyces cerevisiae, are facilitated diffusion transporters. Apparent K(m)-values from high to low affinity, determined from countertransport and initial-uptake experiments, respectively, are: Hxt6 0.9+/-0.2 and 1.4+/-0.1 mM, Hxt7 1.3+/-0.3 and 1.9+/-0.1 mM, Gal2 1.5 and 1.6+/-0.1 mM, Hxt2 2.9+/-0.3 and 4.6+/-0.3 mM, Hxt4 6.2+/-0.5 and 6.2+/-0.3 mM, Hxt3 28.6+/-6.8 and 34.2+/-3.2 mM, and Hxt1 107+/-49 and 129+/-9 mM. From both independent methods, countertransport and initial uptake, the same range of apparent K(m)-values was obtained for each transporter. In contrast to that in human erythrocytes, the facilitated diffusion transport mechanism of glucose in yeast was symmetric. Besides facilitated diffusion there existed in all single glucose transport mutants, except for the HXT1 strain, significant first-order behaviour.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Philipps-University, D-35033 Marburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12702270

Citation

Maier, Andreas, et al. "Characterisation of Glucose Transport in Saccharomyces Cerevisiae With Plasma Membrane Vesicles (countertransport) and Intact Cells (initial Uptake) With Single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 Transporters." FEMS Yeast Research, vol. 2, no. 4, 2002, pp. 539-50.
Maier A, Völker B, Boles E, et al. Characterisation of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS Yeast Res. 2002;2(4):539-50.
Maier, A., Völker, B., Boles, E., & Fuhrmann, G. F. (2002). Characterisation of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS Yeast Research, 2(4), 539-50.
Maier A, et al. Characterisation of Glucose Transport in Saccharomyces Cerevisiae With Plasma Membrane Vesicles (countertransport) and Intact Cells (initial Uptake) With Single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 Transporters. FEMS Yeast Res. 2002;2(4):539-50. PubMed PMID: 12702270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. AU - Maier,Andreas, AU - Völker,Bernhard, AU - Boles,Eckhard, AU - Fuhrmann,Günter Fred, PY - 2003/4/19/pubmed PY - 2003/11/7/medline PY - 2003/4/19/entrez SP - 539 EP - 50 JF - FEMS yeast research JO - FEMS Yeast Res VL - 2 IS - 4 N2 - The yeast glucose transporters Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 and Gal2, individually expressed in an hxt1-7 null mutant strain, demonstrate the phenomenon of countertransport. Thus, these transporters, which are the most important glucose transporters in Saccharomyces cerevisiae, are facilitated diffusion transporters. Apparent K(m)-values from high to low affinity, determined from countertransport and initial-uptake experiments, respectively, are: Hxt6 0.9+/-0.2 and 1.4+/-0.1 mM, Hxt7 1.3+/-0.3 and 1.9+/-0.1 mM, Gal2 1.5 and 1.6+/-0.1 mM, Hxt2 2.9+/-0.3 and 4.6+/-0.3 mM, Hxt4 6.2+/-0.5 and 6.2+/-0.3 mM, Hxt3 28.6+/-6.8 and 34.2+/-3.2 mM, and Hxt1 107+/-49 and 129+/-9 mM. From both independent methods, countertransport and initial uptake, the same range of apparent K(m)-values was obtained for each transporter. In contrast to that in human erythrocytes, the facilitated diffusion transport mechanism of glucose in yeast was symmetric. Besides facilitated diffusion there existed in all single glucose transport mutants, except for the HXT1 strain, significant first-order behaviour. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/12702270/Characterisation_of_glucose_transport_in_Saccharomyces_cerevisiae_with_plasma_membrane_vesicles__countertransport__and_intact_cells__initial_uptake__with_single_Hxt1_Hxt2_Hxt3_Hxt4_Hxt6_Hxt7_or_Gal2_transporters_ DB - PRIME DP - Unbound Medicine ER -