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Determination of in vivo kinetics of the starvation-induced Hxt5 glucose transporter of Saccharomyces cerevisiae.
FEMS Yeast Res. 2002 Aug; 2(3):283-91.FY

Abstract

We have investigated the role and the kinetic properties of the Hxt5 glucose transporter of Saccharomyces cerevisiae. The HXT5 gene was not expressed during growth of the yeast cells in rich medium with glucose or raffinose. However, it became strongly induced during nitrogen or carbon starvation. We have constructed yeast strains constitutively expressing only Hxt5, Hxt1 (low affinity) or Hxt7 (high affinity), but no other glucose transporters. Aerobic fed-batch cultures at quasi steady-state conditions, and aerobic and anaerobic chemostat cultures at steady-state conditions of these strains were used for estimation of the kinetic properties of the individual transporters under in vivo conditions, by investigating the dynamic responses of the strains to changes in extracellular glucose concentration. The K(m) value and the growth properties of the HXT5 single expression strain indicate that Hxt5 is a transporter with intermediate affinity.

Authors+Show Affiliations

Institut für Bioverfahrenstechnik, Universität Stuttgart, Allmandring 31, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12702277

Citation

Buziol, Stefan, et al. "Determination of in Vivo Kinetics of the Starvation-induced Hxt5 Glucose Transporter of Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 2, no. 3, 2002, pp. 283-91.
Buziol S, Becker J, Baumeister A, et al. Determination of in vivo kinetics of the starvation-induced Hxt5 glucose transporter of Saccharomyces cerevisiae. FEMS Yeast Res. 2002;2(3):283-91.
Buziol, S., Becker, J., Baumeister, A., Jung, S., Mauch, K., Reuss, M., & Boles, E. (2002). Determination of in vivo kinetics of the starvation-induced Hxt5 glucose transporter of Saccharomyces cerevisiae. FEMS Yeast Research, 2(3), 283-91.
Buziol S, et al. Determination of in Vivo Kinetics of the Starvation-induced Hxt5 Glucose Transporter of Saccharomyces Cerevisiae. FEMS Yeast Res. 2002;2(3):283-91. PubMed PMID: 12702277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of in vivo kinetics of the starvation-induced Hxt5 glucose transporter of Saccharomyces cerevisiae. AU - Buziol,Stefan, AU - Becker,Jessica, AU - Baumeister,Anja, AU - Jung,Susanne, AU - Mauch,Klaus, AU - Reuss,Matthias, AU - Boles,Eckhard, PY - 2003/4/19/pubmed PY - 2003/11/7/medline PY - 2003/4/19/entrez SP - 283 EP - 91 JF - FEMS yeast research JO - FEMS Yeast Res VL - 2 IS - 3 N2 - We have investigated the role and the kinetic properties of the Hxt5 glucose transporter of Saccharomyces cerevisiae. The HXT5 gene was not expressed during growth of the yeast cells in rich medium with glucose or raffinose. However, it became strongly induced during nitrogen or carbon starvation. We have constructed yeast strains constitutively expressing only Hxt5, Hxt1 (low affinity) or Hxt7 (high affinity), but no other glucose transporters. Aerobic fed-batch cultures at quasi steady-state conditions, and aerobic and anaerobic chemostat cultures at steady-state conditions of these strains were used for estimation of the kinetic properties of the individual transporters under in vivo conditions, by investigating the dynamic responses of the strains to changes in extracellular glucose concentration. The K(m) value and the growth properties of the HXT5 single expression strain indicate that Hxt5 is a transporter with intermediate affinity. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/12702277/Determination_of_in_vivo_kinetics_of_the_starvation_induced_Hxt5_glucose_transporter_of_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1016/S1567-1356(02)00113-7 DB - PRIME DP - Unbound Medicine ER -