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Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae.
FEMS Yeast Res. 2001 Jul; 1(2):139-49.FY

Abstract

The tendency of Saccharomyces cerevisiae to favor alcoholic fermentation over respiration is a complication in aerobic, biomass-directed applications of this yeast. Overproduction of Hap4p, a positive transcriptional regulator of genes involved in respiratory metabolism, has been reported to positively affect the balance between respiration and fermentation in aerobic glucose-grown batch cultures. In this study, the effects of HAP4 overexpression have been quantified in the prototrophic S. cerevisiae strain CEN.PK 113-7D under a variety of growth conditions. In aerobic glucose-limited chemostat cultures, overexpression of HAP4 increased the specific growth rate at which aerobic fermentation set in by about 10% relative to the isogenic wild-type. Upon relief of glucose-limited conditions, the HAP4-overexpressing strain produced slightly less ethanol than the wild-type strain. The effect of Hap4p overproduction was most drastic in aerobic, glucose-grown chemostat cultures in which ammonium was limiting. In such cultures, the biomass yield on glucose was double that of the wild-type.

Authors+Show Affiliations

Kluwer Laboratory of Biotechnology, Delft University of Technology, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12702359

Citation

van Maris, A J., et al. "Modulating the Distribution of Fluxes Among Respiration and Fermentation By Overexpression of HAP4 in Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 1, no. 2, 2001, pp. 139-49.
van Maris AJ, Bakker BM, Brandt M, et al. Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae. FEMS Yeast Res. 2001;1(2):139-49.
van Maris, A. J., Bakker, B. M., Brandt, M., Boorsma, A., Teixeira de Mattos, M. J., Grivell, L. A., Pronk, J. T., & Blom, J. (2001). Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae. FEMS Yeast Research, 1(2), 139-49.
van Maris AJ, et al. Modulating the Distribution of Fluxes Among Respiration and Fermentation By Overexpression of HAP4 in Saccharomyces Cerevisiae. FEMS Yeast Res. 2001;1(2):139-49. PubMed PMID: 12702359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae. AU - van Maris,A J, AU - Bakker,B M, AU - Brandt,M, AU - Boorsma,A, AU - Teixeira de Mattos,M J, AU - Grivell,L A, AU - Pronk,J T, AU - Blom,J, PY - 2003/4/19/pubmed PY - 2003/6/14/medline PY - 2003/4/19/entrez SP - 139 EP - 49 JF - FEMS yeast research JO - FEMS Yeast Res VL - 1 IS - 2 N2 - The tendency of Saccharomyces cerevisiae to favor alcoholic fermentation over respiration is a complication in aerobic, biomass-directed applications of this yeast. Overproduction of Hap4p, a positive transcriptional regulator of genes involved in respiratory metabolism, has been reported to positively affect the balance between respiration and fermentation in aerobic glucose-grown batch cultures. In this study, the effects of HAP4 overexpression have been quantified in the prototrophic S. cerevisiae strain CEN.PK 113-7D under a variety of growth conditions. In aerobic glucose-limited chemostat cultures, overexpression of HAP4 increased the specific growth rate at which aerobic fermentation set in by about 10% relative to the isogenic wild-type. Upon relief of glucose-limited conditions, the HAP4-overexpressing strain produced slightly less ethanol than the wild-type strain. The effect of Hap4p overproduction was most drastic in aerobic, glucose-grown chemostat cultures in which ammonium was limiting. In such cultures, the biomass yield on glucose was double that of the wild-type. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/12702359/Modulating_the_distribution_of_fluxes_among_respiration_and_fermentation_by_overexpression_of_HAP4_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2001.tb00025.x DB - PRIME DP - Unbound Medicine ER -