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Oxidative neurotoxicity in rat cerebral cortex neurons: synergistic effects of H2O2 and NO on apoptosis involving activation of p38 mitogen-activated protein kinase and caspase-3.
J Neurosci Res. 2003 May 15; 72(4):508-19.JN

Abstract

Oxidative stress in the brain has been increasingly associated with the development of numerous human neurological diseases. Microglia, activated upon neuronal injury or inflammatory stimulation, are known to release superoxide anion (*O(2) (-)), hydrogen peroxide (H(2)O(2)), and nitric oxide (NO), thereby further contributing to oxidative neurotoxicity. The reaction of NO and *O(2) (-), forming the toxic peroxynitrite (ONOO(-)), has been proposed to play a pathogenic role in neuronal injury. However, the interactions between H(2)O(2) and NO during oxidative stress, which may promote or diminish cell death, is less clear. In this study, we explored oxidative neurotoxicity induced by H(2)O(2) plus NO in primary cultures of rat cerebral cortex neurons. As the mechanisms may involve reactions between H(2)O(2) and NO, we monitored the production of ONOO(-)and reactive oxygen species (ROS) throughout the experiments. Results indicated that the NO donor S-nitroso-N-acetyl-D, L-penicillamine (SNAP) and H(2)O(2) by themselves elicited neuronal death in a concentration- and time-dependent manner. Sublytic concentrations of H(2)O(2) plus SNAP were sufficient to induce neuronal apoptosis as determined by DNA laddering and fluorescent staining of apoptotic nuclei. Transient ONOO(-)increase was accompanied by rapid H(2)O(2) decay and NO production, whereas ROS slowly decreased following treatment. Furthermore, p38 mitogen-activated protein kinase (MAPK) activation and the cleavage of caspase-3 were observed. Conversely, inhibition of p38 MAPK and caspase-3 significantly reduced apoptotic death induced by H(2)O(2) plus SNAP. These data suggest that H(2)O(2) and NO act synergistically to induce neuronal death through apoptosis in which activation of p38 MAPK and caspase-3 is involved.

Authors+Show Affiliations

Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12704812

Citation

Wang, Jiz-Yuh, et al. "Oxidative Neurotoxicity in Rat Cerebral Cortex Neurons: Synergistic Effects of H2O2 and NO On Apoptosis Involving Activation of P38 Mitogen-activated Protein Kinase and Caspase-3." Journal of Neuroscience Research, vol. 72, no. 4, 2003, pp. 508-19.
Wang JY, Shum AY, Ho YJ, et al. Oxidative neurotoxicity in rat cerebral cortex neurons: synergistic effects of H2O2 and NO on apoptosis involving activation of p38 mitogen-activated protein kinase and caspase-3. J Neurosci Res. 2003;72(4):508-19.
Wang, J. Y., Shum, A. Y., Ho, Y. J., & Wang, J. Y. (2003). Oxidative neurotoxicity in rat cerebral cortex neurons: synergistic effects of H2O2 and NO on apoptosis involving activation of p38 mitogen-activated protein kinase and caspase-3. Journal of Neuroscience Research, 72(4), 508-19.
Wang JY, et al. Oxidative Neurotoxicity in Rat Cerebral Cortex Neurons: Synergistic Effects of H2O2 and NO On Apoptosis Involving Activation of P38 Mitogen-activated Protein Kinase and Caspase-3. J Neurosci Res. 2003 May 15;72(4):508-19. PubMed PMID: 12704812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative neurotoxicity in rat cerebral cortex neurons: synergistic effects of H2O2 and NO on apoptosis involving activation of p38 mitogen-activated protein kinase and caspase-3. AU - Wang,Jiz-Yuh, AU - Shum,Andrew Y C, AU - Ho,Yi-Jung, AU - Wang,Jia-Yi, PY - 2003/4/22/pubmed PY - 2003/6/7/medline PY - 2003/4/22/entrez SP - 508 EP - 19 JF - Journal of neuroscience research JO - J Neurosci Res VL - 72 IS - 4 N2 - Oxidative stress in the brain has been increasingly associated with the development of numerous human neurological diseases. Microglia, activated upon neuronal injury or inflammatory stimulation, are known to release superoxide anion (*O(2) (-)), hydrogen peroxide (H(2)O(2)), and nitric oxide (NO), thereby further contributing to oxidative neurotoxicity. The reaction of NO and *O(2) (-), forming the toxic peroxynitrite (ONOO(-)), has been proposed to play a pathogenic role in neuronal injury. However, the interactions between H(2)O(2) and NO during oxidative stress, which may promote or diminish cell death, is less clear. In this study, we explored oxidative neurotoxicity induced by H(2)O(2) plus NO in primary cultures of rat cerebral cortex neurons. As the mechanisms may involve reactions between H(2)O(2) and NO, we monitored the production of ONOO(-)and reactive oxygen species (ROS) throughout the experiments. Results indicated that the NO donor S-nitroso-N-acetyl-D, L-penicillamine (SNAP) and H(2)O(2) by themselves elicited neuronal death in a concentration- and time-dependent manner. Sublytic concentrations of H(2)O(2) plus SNAP were sufficient to induce neuronal apoptosis as determined by DNA laddering and fluorescent staining of apoptotic nuclei. Transient ONOO(-)increase was accompanied by rapid H(2)O(2) decay and NO production, whereas ROS slowly decreased following treatment. Furthermore, p38 mitogen-activated protein kinase (MAPK) activation and the cleavage of caspase-3 were observed. Conversely, inhibition of p38 MAPK and caspase-3 significantly reduced apoptotic death induced by H(2)O(2) plus SNAP. These data suggest that H(2)O(2) and NO act synergistically to induce neuronal death through apoptosis in which activation of p38 MAPK and caspase-3 is involved. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/12704812/Oxidative_neurotoxicity_in_rat_cerebral_cortex_neurons:_synergistic_effects_of_H2O2_and_NO_on_apoptosis_involving_activation_of_p38_mitogen_activated_protein_kinase_and_caspase_3_ L2 - https://doi.org/10.1002/jnr.10597 DB - PRIME DP - Unbound Medicine ER -