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Immunosensor for detection of Legionella pneumophila using surface plasmon resonance.
Biosens Bioelectron. 2003 May; 18(5-6):605-11.BB

Abstract

Immunosensor using surface plasmon resonance (SPR) onto self-assembled protein G layer was developed for the detection of Legionella pneumophila. A self-assembled protein G layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2) and the activation process for chemical binding between free amine (-NH(2)) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of self-assembled protein G layer on Au substrate and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of self-assembled protein G layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein G were performed by atomic force microscope (AFM). The immunosensor for detection of L. pneumophila using SPR was developed and its detection limit could find up to 10(5) cells/ml.

Authors+Show Affiliations

Oh BK
Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, 100-611, Seoul, South Korea.
Kim YK
No affiliation info available
Lee W
No affiliation info available
Bae YM
No affiliation info available
Lee WH
No affiliation info available
Choi JW
No affiliation info available

MeSH

Biosensing TechniquesCoated Materials, BiocompatibleElectrodesEquipment DesignEquipment Failure AnalysisImmunoassayLegionella pneumophilaMicroscopy, Atomic ForceNerve Tissue ProteinsSurface Plasmon ResonanceSurface Properties

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

12706569

Citation

Oh, Byung-Keun, et al. "Immunosensor for Detection of Legionella Pneumophila Using Surface Plasmon Resonance." Biosensors & Bioelectronics, vol. 18, no. 5-6, 2003, pp. 605-11.
Oh BK, Kim YK, Lee W, et al. Immunosensor for detection of Legionella pneumophila using surface plasmon resonance. Biosens Bioelectron. 2003;18(5-6):605-11.
Oh, B. K., Kim, Y. K., Lee, W., Bae, Y. M., Lee, W. H., & Choi, J. W. (2003). Immunosensor for detection of Legionella pneumophila using surface plasmon resonance. Biosensors & Bioelectronics, 18(5-6), 605-11.
Oh BK, et al. Immunosensor for Detection of Legionella Pneumophila Using Surface Plasmon Resonance. Biosens Bioelectron. 2003;18(5-6):605-11. PubMed PMID: 12706569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosensor for detection of Legionella pneumophila using surface plasmon resonance. AU - Oh,Byung-Keun, AU - Kim,Young-Kee, AU - Lee,Woochang, AU - Bae,Young-Min, AU - Lee,Won-Hong, AU - Choi,Jeong-Woo, PY - 2003/4/23/pubmed PY - 2004/4/14/medline PY - 2003/4/23/entrez SP - 605 EP - 11 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 18 IS - 5-6 N2 - Immunosensor using surface plasmon resonance (SPR) onto self-assembled protein G layer was developed for the detection of Legionella pneumophila. A self-assembled protein G layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2) and the activation process for chemical binding between free amine (-NH(2)) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of self-assembled protein G layer on Au substrate and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of self-assembled protein G layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein G were performed by atomic force microscope (AFM). The immunosensor for detection of L. pneumophila using SPR was developed and its detection limit could find up to 10(5) cells/ml. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/12706569/Immunosensor_for_detection_of_Legionella_pneumophila_using_surface_plasmon_resonance_ DB - PRIME DP - Unbound Medicine ER -