Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.FEBS Lett. 2003 Apr 24; 541(1-3):115-20.FL
Abstract
The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.
MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
12706830
Citation
Wolff, Murielle, et al. "Isoprenoid Biosynthesis Via the Methylerythritol Phosphate Pathway: the (E)-4-hydroxy-3-methylbut-2-enyl Diphosphate Reductase (LytB/IspH) From Escherichia Coli Is a [4Fe-4S] Protein." FEBS Letters, vol. 541, no. 1-3, 2003, pp. 115-20.
Wolff M, Seemann M, Tse Sum Bui B, et al. Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein. FEBS Lett. 2003;541(1-3):115-20.
Wolff, M., Seemann, M., Tse Sum Bui, B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A., & Rohmer, M. (2003). Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein. FEBS Letters, 541(1-3), 115-20.
Wolff M, et al. Isoprenoid Biosynthesis Via the Methylerythritol Phosphate Pathway: the (E)-4-hydroxy-3-methylbut-2-enyl Diphosphate Reductase (LytB/IspH) From Escherichia Coli Is a [4Fe-4S] Protein. FEBS Lett. 2003 Apr 24;541(1-3):115-20. PubMed PMID: 12706830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.
AU - Wolff,Murielle,
AU - Seemann,Myriam,
AU - Tse Sum Bui,Bernadette,
AU - Frapart,Yves,
AU - Tritsch,Denis,
AU - Garcia Estrabot,Ana,
AU - Rodríguez-Concepción,Manuel,
AU - Boronat,Albert,
AU - Marquet,Andrée,
AU - Rohmer,Michel,
PY - 2003/4/23/pubmed
PY - 2003/5/16/medline
PY - 2003/4/23/entrez
SP - 115
EP - 20
JF - FEBS letters
JO - FEBS Lett
VL - 541
IS - 1-3
N2 - The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.
SN - 0014-5793
UR - https://www.unboundmedicine.com/medline/citation/12706830/Isoprenoid_biosynthesis_via_the_methylerythritol_phosphate_pathway:_the__E__4_hydroxy_3_methylbut_2_enyl_diphosphate_reductase__LytB/IspH__from_Escherichia_coli_is_a_[4Fe_4S]_protein_
DB - PRIME
DP - Unbound Medicine
ER -
