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Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode.
Anal Bioanal Chem. 2003 May; 376(2):205-9.AB

Abstract

A nanostructured film electrode, a multi-wall carbon nanotubes (MWNT)-modified glassy carbon electrode (GCE), is described for the simultaneous determination of guanine and adenine. The properties of the MWNT-modified GCE were investigated by scanning electron microscopy (SEM) and cyclic voltammetry. The oxidation peak currents of guanine and adenine increased significantly at the MWNT-modified GCE in contrast to those at the bare GCE. The experimental parameters were optimized and a direct electrochemical method for the simultaneous determination of guanine and adenine was proposed. Using the MWNT-modified GCE, a sensitive and direct electrochemical technique for the measurement of native DNA was also developed, and the value of (G+C)/(A+T) of HCl-digested DNA was detected.

Authors+Show Affiliations

Wu K
Department of Chemistry, Wuhan University, 430072 Wuhan, P.R. China.
Fei J
No affiliation info available
Bai W
No affiliation info available
Hu S
No affiliation info available

MeSH

AdenineAdsorptionAnimalsCattleDNAElectrochemistryElectrodesGuanineHydrogen-Ion ConcentrationNucleic Acid ConformationOrganophosphatesSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12709773

Citation

Wu, Kangbing, et al. "Direct Electrochemistry of DNA, Guanine and Adenine at a Nanostructured Film-modified Electrode." Analytical and Bioanalytical Chemistry, vol. 376, no. 2, 2003, pp. 205-9.
Wu K, Fei J, Bai W, et al. Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Anal Bioanal Chem. 2003;376(2):205-9.
Wu, K., Fei, J., Bai, W., & Hu, S. (2003). Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Analytical and Bioanalytical Chemistry, 376(2), 205-9.
Wu K, et al. Direct Electrochemistry of DNA, Guanine and Adenine at a Nanostructured Film-modified Electrode. Anal Bioanal Chem. 2003;376(2):205-9. PubMed PMID: 12709773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. AU - Wu,Kangbing, AU - Fei,Junjie, AU - Bai,Wen, AU - Hu,Shengshui, Y1 - 2003/04/23/ PY - 2003/01/15/received PY - 2003/02/15/revised PY - 2003/02/24/accepted PY - 2003/4/24/pubmed PY - 2003/11/13/medline PY - 2003/4/24/entrez SP - 205 EP - 9 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 376 IS - 2 N2 - A nanostructured film electrode, a multi-wall carbon nanotubes (MWNT)-modified glassy carbon electrode (GCE), is described for the simultaneous determination of guanine and adenine. The properties of the MWNT-modified GCE were investigated by scanning electron microscopy (SEM) and cyclic voltammetry. The oxidation peak currents of guanine and adenine increased significantly at the MWNT-modified GCE in contrast to those at the bare GCE. The experimental parameters were optimized and a direct electrochemical method for the simultaneous determination of guanine and adenine was proposed. Using the MWNT-modified GCE, a sensitive and direct electrochemical technique for the measurement of native DNA was also developed, and the value of (G+C)/(A+T) of HCl-digested DNA was detected. SN - 1618-2642 UR - https://www.unboundmedicine.com/medline/citation/12709773/Direct_electrochemistry_of_DNA_guanine_and_adenine_at_a_nanostructured_film_modified_electrode_ DB - PRIME DP - Unbound Medicine ER -