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Oxidized LDL autoantibodies are related to apolipoprotein E phenotype, independently of postprandial change in plasma triglycerides and LDL size, among patients with angiographically verified coronary artery disease and healthy controls.
J Biomed Sci. 2003 May-Jun; 10(3):345-51.JB

Abstract

OBJECTIVE

Oxidized low-density lipoprotein (LDL) autoantibodies (oxLDLab), apolipoprotein E (apoE) phenotype, postprandial triglyceride changes and LDL size are suggested to be risk factors for coronary artery disease (CAD). Our aim was to study the interaction between these new risk factors among patients with CAD and healthy controls.

METHODS

oxLDLab from 31 men with angiographically verified CAD and 31 healthy men were analyzed by enzyme-linked immunosorbent assay. Isoelectric focusing and immunoblotting were used for apoE phenotyping. Triglyceride level was measured after 12 h of fasting and 3, 5 and 7 h after a high-fat meal. Nondenaturing gradient gel electrophoresis was used to separate LDL particles according to size.

RESULTS

oxLD- Lab levels increased according to apoE phenotype in the following order: E2 < E3 < E4 (p = 0.004, ANOVA). The postprandial response of triglycerides, the size of LDL particles and the concentration of LDL and high-density lipoprotein (HDL) cholesterol did not differ between apoE phenotypes, and the use of these variables as covariates did not change the statistically significant difference in oxLDLab levels between apoE phenotypes (p = 0.01, ANCOVA). oxLDLab levels did not differ between the patient and control groups.

CONCLUSION

We found an association between apoE allele epsilon2 and decreased levels of oxLDLab, which was independent of the postprandial response of triglycerides, the size of LDL particles and plasma LDL and HDL cholesterol levels. The mechanism by which apoE affects oxidation of LDL remains unknown.

Authors+Show Affiliations

Laboratory of Atherosclerosis Genetics, Centre for Laboratory Medicine, Tampere University Hospital, PO Box 2000, FIN-33521 Tampere, Finland. Saara.Metso@kotiposti.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12711862

Citation

Metso, Saara, et al. "Oxidized LDL Autoantibodies Are Related to Apolipoprotein E Phenotype, Independently of Postprandial Change in Plasma Triglycerides and LDL Size, Among Patients With Angiographically Verified Coronary Artery Disease and Healthy Controls." Journal of Biomedical Science, vol. 10, no. 3, 2003, pp. 345-51.
Metso S, Nikkilä M, Laippala P, et al. Oxidized LDL autoantibodies are related to apolipoprotein E phenotype, independently of postprandial change in plasma triglycerides and LDL size, among patients with angiographically verified coronary artery disease and healthy controls. J Biomed Sci. 2003;10(3):345-51.
Metso, S., Nikkilä, M., Laippala, P., Jaakkola, O., Solakivi, T., & Lehtimäki, T. (2003). Oxidized LDL autoantibodies are related to apolipoprotein E phenotype, independently of postprandial change in plasma triglycerides and LDL size, among patients with angiographically verified coronary artery disease and healthy controls. Journal of Biomedical Science, 10(3), 345-51.
Metso S, et al. Oxidized LDL Autoantibodies Are Related to Apolipoprotein E Phenotype, Independently of Postprandial Change in Plasma Triglycerides and LDL Size, Among Patients With Angiographically Verified Coronary Artery Disease and Healthy Controls. J Biomed Sci. 2003;10(3):345-51. PubMed PMID: 12711862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidized LDL autoantibodies are related to apolipoprotein E phenotype, independently of postprandial change in plasma triglycerides and LDL size, among patients with angiographically verified coronary artery disease and healthy controls. AU - Metso,Saara, AU - Nikkilä,Matti, AU - Laippala,Pekka, AU - Jaakkola,Olli, AU - Solakivi,Tiina, AU - Lehtimäki,Terho, PY - 2002/10/10/received PY - 2003/01/23/accepted PY - 2003/4/25/pubmed PY - 2004/1/22/medline PY - 2003/4/25/entrez SP - 345 EP - 51 JF - Journal of biomedical science JO - J. Biomed. Sci. VL - 10 IS - 3 N2 - OBJECTIVE: Oxidized low-density lipoprotein (LDL) autoantibodies (oxLDLab), apolipoprotein E (apoE) phenotype, postprandial triglyceride changes and LDL size are suggested to be risk factors for coronary artery disease (CAD). Our aim was to study the interaction between these new risk factors among patients with CAD and healthy controls. METHODS: oxLDLab from 31 men with angiographically verified CAD and 31 healthy men were analyzed by enzyme-linked immunosorbent assay. Isoelectric focusing and immunoblotting were used for apoE phenotyping. Triglyceride level was measured after 12 h of fasting and 3, 5 and 7 h after a high-fat meal. Nondenaturing gradient gel electrophoresis was used to separate LDL particles according to size. RESULTS: oxLD- Lab levels increased according to apoE phenotype in the following order: E2 < E3 < E4 (p = 0.004, ANOVA). The postprandial response of triglycerides, the size of LDL particles and the concentration of LDL and high-density lipoprotein (HDL) cholesterol did not differ between apoE phenotypes, and the use of these variables as covariates did not change the statistically significant difference in oxLDLab levels between apoE phenotypes (p = 0.01, ANCOVA). oxLDLab levels did not differ between the patient and control groups. CONCLUSION: We found an association between apoE allele epsilon2 and decreased levels of oxLDLab, which was independent of the postprandial response of triglycerides, the size of LDL particles and plasma LDL and HDL cholesterol levels. The mechanism by which apoE affects oxidation of LDL remains unknown. SN - 1021-7770 UR - https://www.unboundmedicine.com/medline/citation/12711862/Oxidized_LDL_autoantibodies_are_related_to_apolipoprotein_E_phenotype_independently_of_postprandial_change_in_plasma_triglycerides_and_LDL_size_among_patients_with_angiographically_verified_coronary_artery_disease_and_healthy_controls_ L2 - https://medlineplus.gov/coronaryarterydisease.html DB - PRIME DP - Unbound Medicine ER -