Tags

Type your tag names separated by a space and hit enter

Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y.
Am J Clin Nutr. 2003 May; 77(5):1278-86.AJ

Abstract

BACKGROUND

There is little information about the relation between the fatty acid composition of human immune cells and the function of those cells over the habitual range of fatty acid intakes.

OBJECTIVE

The objective of the study was to determine the relation between the fatty acid composition of human peripheral blood mononuclear cell (PBMC) phospholipids and the functions of human immune cells.

DESIGN

One hundred fifty healthy adult subjects provided a fasting blood sample. The phagocytic and oxidative burst activities of monocytes and neutrophils were measured in whole blood. PBMCs were isolated and used to measure lymphocyte proliferation in response to the T cell mitogen concanavalin A and the production of cytokines in response to concanavalin A or bacterial lipopolysaccharide. The fatty acid composition of plasma and PBMC phospholipids was determined.

RESULTS

Wide variations in fatty acid composition of PBMC phospholipids and immune cell functions were identified among the subjects. The proportions of total polyunsaturated fatty acids (PUFAs), of total n-6 and n-3 PUFAs, and of several individual PUFAs in PBMC phospholipids were positively correlated with phagocytosis by neutrophils and monocytes, neutrophil oxidative burst, lymphocyte proliferation, and interferon gamma production. The ratios of saturated fatty acids to PUFAs and of n-6 to n-3 PUFAs were negatively correlated with these same immune functions. The relation of PBMC fatty acid composition to monocyte oxidative burst was the reverse of its relation to monocyte phagocytosis and neutrophil oxidative burst.

CONCLUSION

Variations in the fatty acid composition of PBMC phospholipids account for some of the variability in immune cell functions among healthy adults.

Authors+Show Affiliations

Institute of Human Nutrition, University of Southampton, Southampton, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12716683

Citation

Kew, Samantha, et al. "Relation Between the Fatty Acid Composition of Peripheral Blood Mononuclear Cells and Measures of Immune Cell Function in Healthy, Free-living Subjects Aged 25-72 Y." The American Journal of Clinical Nutrition, vol. 77, no. 5, 2003, pp. 1278-86.
Kew S, Banerjee T, Minihane AM, et al. Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y. Am J Clin Nutr. 2003;77(5):1278-86.
Kew, S., Banerjee, T., Minihane, A. M., Finnegan, Y. E., Williams, C. M., & Calder, P. C. (2003). Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y. The American Journal of Clinical Nutrition, 77(5), 1278-86.
Kew S, et al. Relation Between the Fatty Acid Composition of Peripheral Blood Mononuclear Cells and Measures of Immune Cell Function in Healthy, Free-living Subjects Aged 25-72 Y. Am J Clin Nutr. 2003;77(5):1278-86. PubMed PMID: 12716683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y. AU - Kew,Samantha, AU - Banerjee,Tapati, AU - Minihane,Anne M, AU - Finnegan,Yvonne E, AU - Williams,Christine M, AU - Calder,Philip C, PY - 2003/4/30/pubmed PY - 2003/5/21/medline PY - 2003/4/30/entrez SP - 1278 EP - 86 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 77 IS - 5 N2 - BACKGROUND: There is little information about the relation between the fatty acid composition of human immune cells and the function of those cells over the habitual range of fatty acid intakes. OBJECTIVE: The objective of the study was to determine the relation between the fatty acid composition of human peripheral blood mononuclear cell (PBMC) phospholipids and the functions of human immune cells. DESIGN: One hundred fifty healthy adult subjects provided a fasting blood sample. The phagocytic and oxidative burst activities of monocytes and neutrophils were measured in whole blood. PBMCs were isolated and used to measure lymphocyte proliferation in response to the T cell mitogen concanavalin A and the production of cytokines in response to concanavalin A or bacterial lipopolysaccharide. The fatty acid composition of plasma and PBMC phospholipids was determined. RESULTS: Wide variations in fatty acid composition of PBMC phospholipids and immune cell functions were identified among the subjects. The proportions of total polyunsaturated fatty acids (PUFAs), of total n-6 and n-3 PUFAs, and of several individual PUFAs in PBMC phospholipids were positively correlated with phagocytosis by neutrophils and monocytes, neutrophil oxidative burst, lymphocyte proliferation, and interferon gamma production. The ratios of saturated fatty acids to PUFAs and of n-6 to n-3 PUFAs were negatively correlated with these same immune functions. The relation of PBMC fatty acid composition to monocyte oxidative burst was the reverse of its relation to monocyte phagocytosis and neutrophil oxidative burst. CONCLUSION: Variations in the fatty acid composition of PBMC phospholipids account for some of the variability in immune cell functions among healthy adults. SN - 0002-9165 UR - https://www.unboundmedicine.com/medline/citation/12716683/Relation_between_the_fatty_acid_composition_of_peripheral_blood_mononuclear_cells_and_measures_of_immune_cell_function_in_healthy_free_living_subjects_aged_25_72_y_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/77.5.1278 DB - PRIME DP - Unbound Medicine ER -