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Classical and latent class analysis evaluation of sputum polymerase chain reaction and urine antigen testing for diagnosis of pneumococcal pneumonia in adults.
J Infect Dis. 2003 May 01; 187(9):1416-23.JI

Abstract

Diagnosis of pneumococcal pneumonia is complicated by the lack of a diagnostic reference standard that is highly sensitive and specific. Latent class analysis (LCA) is a mathematical technique that relates an unobserved ("latent") infection to multiple diagnostic test results by use of a statistical model. We used classical analysis and LCA to evaluate the sensitivity and specificity of blood culture, sputum Gram stain, sputum polymerase chain reaction (PCR), and urine antigen testing for diagnosing pneumococcal pneumonia among 149 adults with community-acquired pneumonia. On the basis of LCA models, sensitivity of autolysin PCR and pneumolysin PCR was 82% and 89%, respectively, but specificity was low, 38% and 27%, respectively. For urine antigen testing, sensitivity was 77%-78%, and specificity was 67%-71%. Results of the LCA models were comparable with those obtained from classical analysis. LCA may be useful for diagnostic test evaluation and for determining the prevalence of pneumococcal infection in epidemiological studies of community-acquired pneumonia and in vaccine efficacy trials.

Authors+Show Affiliations

Respiratory Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. Jbutler@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12717623

Citation

Butler, Jay C., et al. "Classical and Latent Class Analysis Evaluation of Sputum Polymerase Chain Reaction and Urine Antigen Testing for Diagnosis of Pneumococcal Pneumonia in Adults." The Journal of Infectious Diseases, vol. 187, no. 9, 2003, pp. 1416-23.
Butler JC, Bosshardt SC, Phelan M, et al. Classical and latent class analysis evaluation of sputum polymerase chain reaction and urine antigen testing for diagnosis of pneumococcal pneumonia in adults. J Infect Dis. 2003;187(9):1416-23.
Butler, J. C., Bosshardt, S. C., Phelan, M., Moroney, S. M., Tondella, M. L., Farley, M. M., Schuchat, A., & Fields, B. S. (2003). Classical and latent class analysis evaluation of sputum polymerase chain reaction and urine antigen testing for diagnosis of pneumococcal pneumonia in adults. The Journal of Infectious Diseases, 187(9), 1416-23.
Butler JC, et al. Classical and Latent Class Analysis Evaluation of Sputum Polymerase Chain Reaction and Urine Antigen Testing for Diagnosis of Pneumococcal Pneumonia in Adults. J Infect Dis. 2003 May 1;187(9):1416-23. PubMed PMID: 12717623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Classical and latent class analysis evaluation of sputum polymerase chain reaction and urine antigen testing for diagnosis of pneumococcal pneumonia in adults. AU - Butler,Jay C, AU - Bosshardt,Stephen C, AU - Phelan,Maureen, AU - Moroney,Shannon M, AU - Tondella,M Lucia, AU - Farley,Monica M, AU - Schuchat,Anne, AU - Fields,Barry S, Y1 - 2003/04/09/ PY - 2002/10/18/received PY - 2003/01/14/accepted PY - 2003/4/30/pubmed PY - 2003/6/5/medline PY - 2003/4/30/entrez SP - 1416 EP - 23 JF - The Journal of infectious diseases JO - J Infect Dis VL - 187 IS - 9 N2 - Diagnosis of pneumococcal pneumonia is complicated by the lack of a diagnostic reference standard that is highly sensitive and specific. Latent class analysis (LCA) is a mathematical technique that relates an unobserved ("latent") infection to multiple diagnostic test results by use of a statistical model. We used classical analysis and LCA to evaluate the sensitivity and specificity of blood culture, sputum Gram stain, sputum polymerase chain reaction (PCR), and urine antigen testing for diagnosing pneumococcal pneumonia among 149 adults with community-acquired pneumonia. On the basis of LCA models, sensitivity of autolysin PCR and pneumolysin PCR was 82% and 89%, respectively, but specificity was low, 38% and 27%, respectively. For urine antigen testing, sensitivity was 77%-78%, and specificity was 67%-71%. Results of the LCA models were comparable with those obtained from classical analysis. LCA may be useful for diagnostic test evaluation and for determining the prevalence of pneumococcal infection in epidemiological studies of community-acquired pneumonia and in vaccine efficacy trials. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/12717623/Classical_and_latent_class_analysis_evaluation_of_sputum_polymerase_chain_reaction_and_urine_antigen_testing_for_diagnosis_of_pneumococcal_pneumonia_in_adults_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/374623 DB - PRIME DP - Unbound Medicine ER -