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Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry.
J Mass Spectrom. 2003 Apr; 38(4):427-37.JM

Abstract

Structural characterization of arabinoxylans from wheat by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry using a Q-TOF mass analyser (ESI-Q-TOF) or an ion trap (IT) mass analyser is presented. An arabinoxylan sample digested with endoxylanase A was analysed using MALDI-TOF mass spectrometry (MS), resulting in the identification of molecular ions for structures with up to 22 monosaccharide residues. As the two-component monosaccharides xylose and arabinose are isobaric, structures differing in the number of arabinose branching residues were indistinguishable based on molecular mass and also fragmentation pattern upon collision-induced dissociation (CID). Permethylation followed by ESI-CID analyses using ITMS was performed to obtain structural information regarding the number of arabinose branching residues and their spatial arrangement along the xylose backbone. Analysis of the signal corresponding to an oligomer with six monosaccharide residues showed the presence of at least four isomeric structures differing in degree of branching and position of the branched residue relative to the cleavage site of the enzyme. This is the first demonstration of the use of ESI-ITMS for the structural characterization of arabinoxylan mixtures.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12717755

Citation

Matamoros Fernández, Lobvi E., et al. "Characterization of Plant Oligosaccharides By Matrix-assisted Laser Desorption/ionization and Electrospray Mass Spectrometry." Journal of Mass Spectrometry : JMS, vol. 38, no. 4, 2003, pp. 427-37.
Matamoros Fernández LE, Obel N, Scheller HV, et al. Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry. J Mass Spectrom. 2003;38(4):427-37.
Matamoros Fernández, L. E., Obel, N., Scheller, H. V., & Roepstorff, P. (2003). Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry. Journal of Mass Spectrometry : JMS, 38(4), 427-37.
Matamoros Fernández LE, et al. Characterization of Plant Oligosaccharides By Matrix-assisted Laser Desorption/ionization and Electrospray Mass Spectrometry. J Mass Spectrom. 2003;38(4):427-37. PubMed PMID: 12717755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of plant oligosaccharides by matrix-assisted laser desorption/ionization and electrospray mass spectrometry. AU - Matamoros Fernández,Lobvi E, AU - Obel,Nicolai, AU - Scheller,Henrik Vibe, AU - Roepstorff,Peter, PY - 2003/4/30/pubmed PY - 2003/7/25/medline PY - 2003/4/30/entrez SP - 427 EP - 37 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 38 IS - 4 N2 - Structural characterization of arabinoxylans from wheat by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry using a Q-TOF mass analyser (ESI-Q-TOF) or an ion trap (IT) mass analyser is presented. An arabinoxylan sample digested with endoxylanase A was analysed using MALDI-TOF mass spectrometry (MS), resulting in the identification of molecular ions for structures with up to 22 monosaccharide residues. As the two-component monosaccharides xylose and arabinose are isobaric, structures differing in the number of arabinose branching residues were indistinguishable based on molecular mass and also fragmentation pattern upon collision-induced dissociation (CID). Permethylation followed by ESI-CID analyses using ITMS was performed to obtain structural information regarding the number of arabinose branching residues and their spatial arrangement along the xylose backbone. Analysis of the signal corresponding to an oligomer with six monosaccharide residues showed the presence of at least four isomeric structures differing in degree of branching and position of the branched residue relative to the cleavage site of the enzyme. This is the first demonstration of the use of ESI-ITMS for the structural characterization of arabinoxylan mixtures. SN - 1076-5174 UR - https://www.unboundmedicine.com/medline/citation/12717755/Characterization_of_plant_oligosaccharides_by_matrix_assisted_laser_desorption/ionization_and_electrospray_mass_spectrometry_ L2 - https://doi.org/10.1002/jms.455 DB - PRIME DP - Unbound Medicine ER -