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Analysis of glycopeptide antibiotics using micellar electrokinetic chromatography and borate complexation.
Biomed Chromatogr. 2003 Mar-Apr; 17(2-3):172-81.BC

Abstract

Micellar electrokinetic chromatography (MEKC) was investigated as a technique for the separation and analysis of the following related glycopeptide antibiotics: alpha-avoparcin, beta-avoparcin, ristocetin A, ristocetin B and vancomycin. Sodium dodecyl sulfate (SDS) micelles were employed as the pseudostationary phase in conjunction with borate or CHES buffers at pH 9.2. A complete separation of the glycopeptides was achieved only when two separation mechanisms were employed simultaneously: (i) differential partitioning of the glycopeptides into SDS micelles; and (ii) differential complexation of the glycopeptides with the borate anion from the borate buffer. Quantitatively, linearity was confirmed for each antibiotic from 0.5 to 40 ppm, with correlation coefficients (r(2)) ranging from 0.9996 (vancomycin and beta-avoparcin) to 0.9986 (alpha-avoparcin). Detection limits ranging from 0.01 ppm (vancomycin) to 0.2 ppm (avoparcin) were achieved, and the mean recovery of avoparcin at the 10 ppm level was 99.2%.

Authors+Show Affiliations

Department of Chemistry, Villanova University, Villanova, PA 19085-1699, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12717807

Citation

Lucas, Carmelle, et al. "Analysis of Glycopeptide Antibiotics Using Micellar Electrokinetic Chromatography and Borate Complexation." Biomedical Chromatography : BMC, vol. 17, no. 2-3, 2003, pp. 172-81.
Lucas C, Foley JP, Ahuja ES. Analysis of glycopeptide antibiotics using micellar electrokinetic chromatography and borate complexation. Biomed Chromatogr. 2003;17(2-3):172-81.
Lucas, C., Foley, J. P., & Ahuja, E. S. (2003). Analysis of glycopeptide antibiotics using micellar electrokinetic chromatography and borate complexation. Biomedical Chromatography : BMC, 17(2-3), 172-81.
Lucas C, Foley JP, Ahuja ES. Analysis of Glycopeptide Antibiotics Using Micellar Electrokinetic Chromatography and Borate Complexation. Biomed Chromatogr. 2003 Mar-Apr;17(2-3):172-81. PubMed PMID: 12717807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of glycopeptide antibiotics using micellar electrokinetic chromatography and borate complexation. AU - Lucas,Carmelle, AU - Foley,Joe P, AU - Ahuja,Eric S, PY - 2003/4/30/pubmed PY - 2003/12/10/medline PY - 2003/4/30/entrez SP - 172 EP - 81 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 17 IS - 2-3 N2 - Micellar electrokinetic chromatography (MEKC) was investigated as a technique for the separation and analysis of the following related glycopeptide antibiotics: alpha-avoparcin, beta-avoparcin, ristocetin A, ristocetin B and vancomycin. Sodium dodecyl sulfate (SDS) micelles were employed as the pseudostationary phase in conjunction with borate or CHES buffers at pH 9.2. A complete separation of the glycopeptides was achieved only when two separation mechanisms were employed simultaneously: (i) differential partitioning of the glycopeptides into SDS micelles; and (ii) differential complexation of the glycopeptides with the borate anion from the borate buffer. Quantitatively, linearity was confirmed for each antibiotic from 0.5 to 40 ppm, with correlation coefficients (r(2)) ranging from 0.9996 (vancomycin and beta-avoparcin) to 0.9986 (alpha-avoparcin). Detection limits ranging from 0.01 ppm (vancomycin) to 0.2 ppm (avoparcin) were achieved, and the mean recovery of avoparcin at the 10 ppm level was 99.2%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/12717807/Analysis_of_glycopeptide_antibiotics_using_micellar_electrokinetic_chromatography_and_borate_complexation_ DB - PRIME DP - Unbound Medicine ER -