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Downregulation of electroacupuncture at ST36 on TNF-alpha in rats with ulcerative colitis.
World J Gastroenterol. 2003 May; 9(5):1028-33.WJ

Abstract

AIM

To investigate the regulatory effect of electroacupuncture (EA) at Zusanli (ST36) on tumor necrosis factor-alpha (TNF-alpha) in rats with ulcerative colitis (UC), and further elucidate the therapeutic mechanism of EA on UC.

METHODS

Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8): normal control group; UC control group; UC+ST36 group and UC+non-acupoint group. A solution containing ethanol and 2,4,6-trinitrobenzenesulfonic acid (TNBS) was instilled into the distal colon in the rat (at a dose of 100 mg/kg) to set up UC rat model. Rats in wakefulness state of UC+ST36 group were stimulated at ST36 by EA once a day, while those of UC+non-acupoint group were done at 0.5 cm beside ST36. After 10 d treatment, all rats were sacrificed simultaneously. Colon musocal inflammation and damage were assessed by measuring colon mass, morphologic damage score, colonic myeloperoxidase enzyme (MPO) activity, serum TNF-alpha and colonic TNF-alpha mRNA level. Morphologic damage score was examined under stereomicroscope. Colonic MPO activity was measured by spectrophotometer method. Serum TNF-alpha concentration was determined by radioimmunoassay (RIA). Colonic TNF-alpha mRNA expression level was analyzed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR).

RESULTS

Ratio of colonic mass/body mass (m(C)/m(B)) and activity of colonic MPO (microkat/g tissue) markedly increased (8.5+/-2.6 vs 2.5+/-0.4; 145+/-25 vs 24+/-8, P<0.01 vs normal control group). Compared with normal control rats, serum TNF-alpha and colonic TNF-alpha mRNA level in UC control group were increased 2.5 fold (2 278+/-170 vs 894+/-248, P<0.01) and 4.3 fold (0.98+/-0.11 vs 0.23+/-0.11, P<0.01), respectively. After EA at ST36, m(C)/m(B) and MPO activity were reduced significantly (5.3+/-2.0 vs 8.5+/-2.6; 104+/-36 vs 145+/-25, P<0.01, 0.05) compared with those of UC control group. Serum TNF-alpha and colonic TNF-alpha mRNA level were inhibited by EA stimulation at ST36 (P<0.01). The inhibitory rate was 16 % and 44 %, respectively. Morphologic damage score was also increased markedly in rat with UC (P<0.01), whereas it was decreased by EA at ST36 (P<0.05). There was no significant difference between UC control group and UC+EA at non-acupoint (P>0.05). Furthermore, these parameters were highly correlated with each other (P<0.01).

CONCLUSION

Serum TNF-alpha concentration and colonic TNF-alpha mRNA expression level are increased significantly in UC rats in correlation with the severity of disease. It indicates that TNF-alpha is closely involved in the immune abnormalities and inflammatory responses in UC. EA at ST36 has therapeutic effect on UC by downregulating serum TNF-alpha and colonic TNF-alpha mRNA expression. High levels of TNF-alpha and its corresponding mRNA expression seem to be implicated in the pathogenesis of UC.

Authors+Show Affiliations

Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shanxi Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12717850

Citation

Tian, Li, et al. "Downregulation of Electroacupuncture at ST36 On TNF-alpha in Rats With Ulcerative Colitis." World Journal of Gastroenterology, vol. 9, no. 5, 2003, pp. 1028-33.
Tian L, Huang YX, Tian M, et al. Downregulation of electroacupuncture at ST36 on TNF-alpha in rats with ulcerative colitis. World J Gastroenterol. 2003;9(5):1028-33.
Tian, L., Huang, Y. X., Tian, M., Gao, W., & Chang, Q. (2003). Downregulation of electroacupuncture at ST36 on TNF-alpha in rats with ulcerative colitis. World Journal of Gastroenterology, 9(5), 1028-33.
Tian L, et al. Downregulation of Electroacupuncture at ST36 On TNF-alpha in Rats With Ulcerative Colitis. World J Gastroenterol. 2003;9(5):1028-33. PubMed PMID: 12717850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of electroacupuncture at ST36 on TNF-alpha in rats with ulcerative colitis. AU - Tian,Li, AU - Huang,Yu-Xin, AU - Tian,Min, AU - Gao,Wei, AU - Chang,Qing, PY - 2003/4/30/pubmed PY - 2003/7/15/medline PY - 2003/4/30/entrez SP - 1028 EP - 33 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 9 IS - 5 N2 - AIM: To investigate the regulatory effect of electroacupuncture (EA) at Zusanli (ST36) on tumor necrosis factor-alpha (TNF-alpha) in rats with ulcerative colitis (UC), and further elucidate the therapeutic mechanism of EA on UC. METHODS: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8): normal control group; UC control group; UC+ST36 group and UC+non-acupoint group. A solution containing ethanol and 2,4,6-trinitrobenzenesulfonic acid (TNBS) was instilled into the distal colon in the rat (at a dose of 100 mg/kg) to set up UC rat model. Rats in wakefulness state of UC+ST36 group were stimulated at ST36 by EA once a day, while those of UC+non-acupoint group were done at 0.5 cm beside ST36. After 10 d treatment, all rats were sacrificed simultaneously. Colon musocal inflammation and damage were assessed by measuring colon mass, morphologic damage score, colonic myeloperoxidase enzyme (MPO) activity, serum TNF-alpha and colonic TNF-alpha mRNA level. Morphologic damage score was examined under stereomicroscope. Colonic MPO activity was measured by spectrophotometer method. Serum TNF-alpha concentration was determined by radioimmunoassay (RIA). Colonic TNF-alpha mRNA expression level was analyzed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Ratio of colonic mass/body mass (m(C)/m(B)) and activity of colonic MPO (microkat/g tissue) markedly increased (8.5+/-2.6 vs 2.5+/-0.4; 145+/-25 vs 24+/-8, P<0.01 vs normal control group). Compared with normal control rats, serum TNF-alpha and colonic TNF-alpha mRNA level in UC control group were increased 2.5 fold (2 278+/-170 vs 894+/-248, P<0.01) and 4.3 fold (0.98+/-0.11 vs 0.23+/-0.11, P<0.01), respectively. After EA at ST36, m(C)/m(B) and MPO activity were reduced significantly (5.3+/-2.0 vs 8.5+/-2.6; 104+/-36 vs 145+/-25, P<0.01, 0.05) compared with those of UC control group. Serum TNF-alpha and colonic TNF-alpha mRNA level were inhibited by EA stimulation at ST36 (P<0.01). The inhibitory rate was 16 % and 44 %, respectively. Morphologic damage score was also increased markedly in rat with UC (P<0.01), whereas it was decreased by EA at ST36 (P<0.05). There was no significant difference between UC control group and UC+EA at non-acupoint (P>0.05). Furthermore, these parameters were highly correlated with each other (P<0.01). CONCLUSION: Serum TNF-alpha concentration and colonic TNF-alpha mRNA expression level are increased significantly in UC rats in correlation with the severity of disease. It indicates that TNF-alpha is closely involved in the immune abnormalities and inflammatory responses in UC. EA at ST36 has therapeutic effect on UC by downregulating serum TNF-alpha and colonic TNF-alpha mRNA expression. High levels of TNF-alpha and its corresponding mRNA expression seem to be implicated in the pathogenesis of UC. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/12717850/Downregulation_of_electroacupuncture_at_ST36_on_TNF_alpha_in_rats_with_ulcerative_colitis_ L2 - http://www.wjgnet.com/1007-9327/full/v9/i5/1028.htm DB - PRIME DP - Unbound Medicine ER -