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Routine analysis of amphetamine and methamphetamine in biological materials by gas chromatography-mass spectrometry and on-column derivatization.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jun 05; 789(1):65-71.JC

Abstract

A simple determination method of amphetamine (AP) and methamphetamine (MA) in biological materials was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). AP and MA in biological materials were adsorbed on the surface of Extrelut and then extracted and derivatized simultaneously on the Extrelut column. AP and MA were derivatized to the N-propoxycarbonyl derivatives using propylchloroformate. Pentadeuterated MA was used as an internal standard. The recoveries of AP and MA from urine were 88.2 and 92.5%, and those from blood were 89.7 and 90.3%, respectively. The calibration curves showed linearity in the range of 12.5-2000 ng/ml (ng/g) for AP and MA in urine and blood, and 0.25-20 ng/mg in hair. When urine samples containing two different concentrations (200 and 1000 ng/ml) of AP and MA, blood samples containing two different concentrations (200 and 1000 ng/g) of AP and MA, hair samples containing two different concentrations (0.5 and 5.0 ng/mg) of AP and MA, the coefficients of variation of intra-day and inter-day were 0.68-3.60% in urine, 0.42-4.58% in blood, and 1.20-13.1% in hair. Furthermore, this proposed method was applied to a medico-legal case of MA intoxication.

Authors+Show Affiliations

Department of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551, Hiroshima, Japan. nishidam@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12726844

Citation

Nishida, Manami, et al. "Routine Analysis of Amphetamine and Methamphetamine in Biological Materials By Gas Chromatography-mass Spectrometry and On-column Derivatization." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 789, no. 1, 2003, pp. 65-71.
Nishida M, Namera A, Yashiki M, et al. Routine analysis of amphetamine and methamphetamine in biological materials by gas chromatography-mass spectrometry and on-column derivatization. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;789(1):65-71.
Nishida, M., Namera, A., Yashiki, M., & Kojima, T. (2003). Routine analysis of amphetamine and methamphetamine in biological materials by gas chromatography-mass spectrometry and on-column derivatization. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 789(1), 65-71.
Nishida M, et al. Routine Analysis of Amphetamine and Methamphetamine in Biological Materials By Gas Chromatography-mass Spectrometry and On-column Derivatization. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jun 5;789(1):65-71. PubMed PMID: 12726844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Routine analysis of amphetamine and methamphetamine in biological materials by gas chromatography-mass spectrometry and on-column derivatization. AU - Nishida,Manami, AU - Namera,Akira, AU - Yashiki,Mikio, AU - Kojima,Tohru, PY - 2003/5/3/pubmed PY - 2004/2/11/medline PY - 2003/5/3/entrez SP - 65 EP - 71 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 789 IS - 1 N2 - A simple determination method of amphetamine (AP) and methamphetamine (MA) in biological materials was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). AP and MA in biological materials were adsorbed on the surface of Extrelut and then extracted and derivatized simultaneously on the Extrelut column. AP and MA were derivatized to the N-propoxycarbonyl derivatives using propylchloroformate. Pentadeuterated MA was used as an internal standard. The recoveries of AP and MA from urine were 88.2 and 92.5%, and those from blood were 89.7 and 90.3%, respectively. The calibration curves showed linearity in the range of 12.5-2000 ng/ml (ng/g) for AP and MA in urine and blood, and 0.25-20 ng/mg in hair. When urine samples containing two different concentrations (200 and 1000 ng/ml) of AP and MA, blood samples containing two different concentrations (200 and 1000 ng/g) of AP and MA, hair samples containing two different concentrations (0.5 and 5.0 ng/mg) of AP and MA, the coefficients of variation of intra-day and inter-day were 0.68-3.60% in urine, 0.42-4.58% in blood, and 1.20-13.1% in hair. Furthermore, this proposed method was applied to a medico-legal case of MA intoxication. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12726844/Routine_analysis_of_amphetamine_and_methamphetamine_in_biological_materials_by_gas_chromatography_mass_spectrometry_and_on_column_derivatization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023203000692 DB - PRIME DP - Unbound Medicine ER -