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Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae).
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Apr; 134(4):481-90.CB

Abstract

The physiology of the gut lumen of the red flour beetle, T. castaneum, was studied to determine the conditions for optimal protein hydrolysis. Although the pH of gut lumen extracts from T. castaneum was 6.5, maximum hydrolysis of casein by gut proteinases occurred at pH 4.2. The synthetic substrate N-alpha-benzoyl-DL-arginine-rho-nitroanilide was hydrolyzed by T. castaneum gut proteinases in both acidic and alkaline buffers, whereas hydrolysis of N-succinyl-ala-ala-pro-phe rho-nitroanilide occurred in alkaline buffer. Inhibitors of T. castaneum digestive proteinases were examined to identify potential biopesticides for incorporation in transgenic seed. Cysteine proteinase inhibitors from potato, Job's tears, and sea anemone (equistatin) were effective inhibitors of in vitro casein hydrolysis by T. castaneum proteinases. Other inhibitors of T. castaneum proteinases included leupeptin, L-trans-epoxysuccinylleucylamido [4-guanidino] butane (E-64), tosyl-L-lysine chloromethyl ketone, and antipain. Casein hydrolysis was inhibited weakly by chymostatin, N-tosyl-L-phenylalanine chloromethyl ketone, and soybean trypsin inhibitor (Kunitz). The soybean trypsin inhibitor had no significant effect on growth when it was bioassayed alone, but it was effective when used in combination with potato cysteine proteinase inhibitor. In other bioassays with single inhibitors, larval growth was suppressed by the cysteine proteinase inhibitors from potato, Job's tears, or sea anemone. Levels of inhibition were similar to that observed with E-64, although the moles of proteinaceous inhibitor tested were approximately 1000-fold less. These proteinaceous inhibitors are promising candidates for transgenic seed technology to reduce seed damage by T. castaneum.

Authors+Show Affiliations

USDA ARS Grain Marketing and Production Research Center, 1515 College Ave., Manhattan, KS 66502, USA. bso@ksu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12727298

Citation

Oppert, B, et al. "Effects of Proteinase Inhibitors On Digestive Proteinases and Growth of the Red Flour Beetle, Tribolium Castaneum (Herbst) (Coleoptera: Tenebrionidae)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 134, no. 4, 2003, pp. 481-90.
Oppert B, Morgan TD, Hartzer K, et al. Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Comp Biochem Physiol C Toxicol Pharmacol. 2003;134(4):481-90.
Oppert, B., Morgan, T. D., Hartzer, K., Lenarcic, B., Galesa, K., Brzin, J., Turk, V., Yoza, K., Ohtsubo, K., & Kramer, K. J. (2003). Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 134(4), 481-90.
Oppert B, et al. Effects of Proteinase Inhibitors On Digestive Proteinases and Growth of the Red Flour Beetle, Tribolium Castaneum (Herbst) (Coleoptera: Tenebrionidae). Comp Biochem Physiol C Toxicol Pharmacol. 2003;134(4):481-90. PubMed PMID: 12727298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). AU - Oppert,B, AU - Morgan,T D, AU - Hartzer,K, AU - Lenarcic,B, AU - Galesa,K, AU - Brzin,J, AU - Turk,V, AU - Yoza,K, AU - Ohtsubo,K, AU - Kramer,K J, PY - 2003/5/3/pubmed PY - 2004/1/22/medline PY - 2003/5/3/entrez SP - 481 EP - 90 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 134 IS - 4 N2 - The physiology of the gut lumen of the red flour beetle, T. castaneum, was studied to determine the conditions for optimal protein hydrolysis. Although the pH of gut lumen extracts from T. castaneum was 6.5, maximum hydrolysis of casein by gut proteinases occurred at pH 4.2. The synthetic substrate N-alpha-benzoyl-DL-arginine-rho-nitroanilide was hydrolyzed by T. castaneum gut proteinases in both acidic and alkaline buffers, whereas hydrolysis of N-succinyl-ala-ala-pro-phe rho-nitroanilide occurred in alkaline buffer. Inhibitors of T. castaneum digestive proteinases were examined to identify potential biopesticides for incorporation in transgenic seed. Cysteine proteinase inhibitors from potato, Job's tears, and sea anemone (equistatin) were effective inhibitors of in vitro casein hydrolysis by T. castaneum proteinases. Other inhibitors of T. castaneum proteinases included leupeptin, L-trans-epoxysuccinylleucylamido [4-guanidino] butane (E-64), tosyl-L-lysine chloromethyl ketone, and antipain. Casein hydrolysis was inhibited weakly by chymostatin, N-tosyl-L-phenylalanine chloromethyl ketone, and soybean trypsin inhibitor (Kunitz). The soybean trypsin inhibitor had no significant effect on growth when it was bioassayed alone, but it was effective when used in combination with potato cysteine proteinase inhibitor. In other bioassays with single inhibitors, larval growth was suppressed by the cysteine proteinase inhibitors from potato, Job's tears, or sea anemone. Levels of inhibition were similar to that observed with E-64, although the moles of proteinaceous inhibitor tested were approximately 1000-fold less. These proteinaceous inhibitors are promising candidates for transgenic seed technology to reduce seed damage by T. castaneum. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/12727298/Effects_of_proteinase_inhibitors_on_digestive_proteinases_and_growth_of_the_red_flour_beetle_Tribolium_castaneum__Herbst___Coleoptera:_Tenebrionidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532045603000425 DB - PRIME DP - Unbound Medicine ER -