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Genomic mutations with amino acid substitutions of circulating hepatitis B virus found in non-B, non-C patients with hepatocellular carcinoma.
Intern Med. 2003 Apr; 42(4):322-30.IM

Abstract

OBJECTIVE

Most hepatocellular carcinoma (HCC) in Japan is caused by chronic infection with hepatitis B virus (HBV) or hepatitis C virus (HCV). HBV DNA has been detected in the serum and liver tissue of some proportion of those patients who are HBs antigen-negative and HCV antibody-negative; i.e., non-B, non-C (NBNC) patients with HCC. We sought to detect HBV DNA in the serum from NBNC HCC cases and to investigate genomic mutations of HBV in seronegative cases.

PATIENTS AND METHODS

The sera from 26 NBNC HCC patients were examined by polymerase chain reaction (PCR) followed by southern blotting for existence of HBV DNA. The precore/core and polymerase regions of the HBV genome in the sera from five seronegative cases were analyzed by direct sequence.

RESULTS

HBV DNA was detected in 17 of 26 patients (65.4%). Demographic factors such as age, gender, anti-HBs positivity, anti-HBc positivity, complication with cirrhosis, and excessive alcohol intake did not affect circulating HBV positivity. Genomic mutations with amino acid substitutions were detected in the polymerase and the precore regions from one of the five cases, and in the core region from four of the five cases.

CONCLUSIONS

PCR-based HBV screening is necessary in patients suffering from liver diseases of unknown etiology, although its etiological importance and benefit of viral elimination have not been established. Genomic mutations in the precore/core and the polymerase region detected in this study might be involved in the lack of HBsAg in NBNC HCC cases.

Authors+Show Affiliations

Department of Internal Medicine, School of Medicine, Keio University, Tokyo.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12729320

Citation

Nakamoto, Nobuhiro, et al. "Genomic Mutations With Amino Acid Substitutions of Circulating Hepatitis B Virus Found in non-B, non-C Patients With Hepatocellular Carcinoma." Internal Medicine (Tokyo, Japan), vol. 42, no. 4, 2003, pp. 322-30.
Nakamoto N, Saito H, Ebinuma H, et al. Genomic mutations with amino acid substitutions of circulating hepatitis B virus found in non-B, non-C patients with hepatocellular carcinoma. Intern Med. 2003;42(4):322-30.
Nakamoto, N., Saito, H., Ebinuma, H., Tada, S., Saito, Y., Kurita, S., Kitamura, K., & Ishii, H. (2003). Genomic mutations with amino acid substitutions of circulating hepatitis B virus found in non-B, non-C patients with hepatocellular carcinoma. Internal Medicine (Tokyo, Japan), 42(4), 322-30.
Nakamoto N, et al. Genomic Mutations With Amino Acid Substitutions of Circulating Hepatitis B Virus Found in non-B, non-C Patients With Hepatocellular Carcinoma. Intern Med. 2003;42(4):322-30. PubMed PMID: 12729320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic mutations with amino acid substitutions of circulating hepatitis B virus found in non-B, non-C patients with hepatocellular carcinoma. AU - Nakamoto,Nobuhiro, AU - Saito,Hidetsugu, AU - Ebinuma,Hirotoshi, AU - Tada,Shinichiro, AU - Saito,Yoshimasa, AU - Kurita,Satoshi, AU - Kitamura,Kumi, AU - Ishii,Hiromasa, PY - 2003/5/6/pubmed PY - 2003/7/31/medline PY - 2003/5/6/entrez SP - 322 EP - 30 JF - Internal medicine (Tokyo, Japan) JO - Intern. Med. VL - 42 IS - 4 N2 - OBJECTIVE: Most hepatocellular carcinoma (HCC) in Japan is caused by chronic infection with hepatitis B virus (HBV) or hepatitis C virus (HCV). HBV DNA has been detected in the serum and liver tissue of some proportion of those patients who are HBs antigen-negative and HCV antibody-negative; i.e., non-B, non-C (NBNC) patients with HCC. We sought to detect HBV DNA in the serum from NBNC HCC cases and to investigate genomic mutations of HBV in seronegative cases. PATIENTS AND METHODS: The sera from 26 NBNC HCC patients were examined by polymerase chain reaction (PCR) followed by southern blotting for existence of HBV DNA. The precore/core and polymerase regions of the HBV genome in the sera from five seronegative cases were analyzed by direct sequence. RESULTS: HBV DNA was detected in 17 of 26 patients (65.4%). Demographic factors such as age, gender, anti-HBs positivity, anti-HBc positivity, complication with cirrhosis, and excessive alcohol intake did not affect circulating HBV positivity. Genomic mutations with amino acid substitutions were detected in the polymerase and the precore regions from one of the five cases, and in the core region from four of the five cases. CONCLUSIONS: PCR-based HBV screening is necessary in patients suffering from liver diseases of unknown etiology, although its etiological importance and benefit of viral elimination have not been established. Genomic mutations in the precore/core and the polymerase region detected in this study might be involved in the lack of HBsAg in NBNC HCC cases. SN - 0918-2918 UR - https://www.unboundmedicine.com/medline/citation/12729320/Genomic_mutations_with_amino_acid_substitutions_of_circulating_hepatitis_B_virus_found_in_non_B_non_C_patients_with_hepatocellular_carcinoma_ L2 - https://joi.jlc.jst.go.jp/JST.Journalarchive/internalmedicine1992/42.322?from=PubMed DB - PRIME DP - Unbound Medicine ER -