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Strawberry anthocyanins are recovered in urine as glucuro- and sulfoconjugates in humans.
J Nutr. 2003 May; 133(5):1296-301.JN

Abstract

Anthocyanins are phenolic compounds widely distributed in fruits and vegetables. Their consumption has been shown to prevent some chronic diseases. Anthocyanin metabolism, however, is still not fully understood. The aim of this work was to evaluate the bioavailability of anthocyanins in humans consuming a meal containing strawberries and to identify possible metabolites in urine. Six healthy volunteers (three women and three men) consumed a meal containing 200 g strawberries (providing 179 micro mol pelargonidin-3-glucoside). Urine samples were collected before and after the meal and rapidly treated by solid-phase extraction. Identification and quantification of anthocyanin metabolites were carried out by HPLC-ESI-MS-MS and HPLC with UV-visible detection, respectively. In addition to pelargonidin-3-glucoside, five anthocyanin metabolites were identified in urine: three monoglucuronides of pelargonidin, one sulfoconjugate of pelargonidin and pelargonidin itself. Total urinary excretion of strawberry anthocyanin metabolites corresponded to 1.80 +/- 0.29% (mean +/- SEM, n = 6) of pelargonidin-3-glucoside ingested. More than 80% of this excretion was related to a monoglucuronide. Four hours after the meal, more than two-thirds of anthocyanin metabolites had been excreted, although urinary excretion of the metabolites continued until the end of the 24-h experiment. This study demonstrated that anthocyanins were glucuro- and sulfo-conjugated in humans and that the main metabolite of strawberry anthocyanins in human urine was a monoglucuronide of pelargonidin.

Authors+Show Affiliations

Felgines C
Laboratoire de Pharmacognosie, Faculté de Pharmacie, 63001 Clermont-Ferrand, France. Catherine.FELGINES@u-clermont1.fr
Talavéra S
No affiliation info available
Gonthier MP
No affiliation info available
Texier O
No affiliation info available
Scalbert A
No affiliation info available
Lamaison JL
No affiliation info available
Rémésy C
No affiliation info available

MeSH

AnthocyaninsChromatography, High Pressure LiquidFragariaFruitGlucuronidesGlycosuriaHumansMetabolic Clearance RateSulfatesVegetables

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12730413

Citation

Felgines, Catherine, et al. "Strawberry Anthocyanins Are Recovered in Urine as Glucuro- and Sulfoconjugates in Humans." The Journal of Nutrition, vol. 133, no. 5, 2003, pp. 1296-301.
Felgines C, Talavéra S, Gonthier MP, et al. Strawberry anthocyanins are recovered in urine as glucuro- and sulfoconjugates in humans. J Nutr. 2003;133(5):1296-301.
Felgines, C., Talavéra, S., Gonthier, M. P., Texier, O., Scalbert, A., Lamaison, J. L., & Rémésy, C. (2003). Strawberry anthocyanins are recovered in urine as glucuro- and sulfoconjugates in humans. The Journal of Nutrition, 133(5), 1296-301.
Felgines C, et al. Strawberry Anthocyanins Are Recovered in Urine as Glucuro- and Sulfoconjugates in Humans. J Nutr. 2003;133(5):1296-301. PubMed PMID: 12730413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strawberry anthocyanins are recovered in urine as glucuro- and sulfoconjugates in humans. AU - Felgines,Catherine, AU - Talavéra,Séverine, AU - Gonthier,Marie-Paule, AU - Texier,Odile, AU - Scalbert,Augustin, AU - Lamaison,Jean-Louis, AU - Rémésy,Christian, PY - 2003/5/6/pubmed PY - 2003/6/25/medline PY - 2003/5/6/entrez SP - 1296 EP - 301 JF - The Journal of nutrition JO - J Nutr VL - 133 IS - 5 N2 - Anthocyanins are phenolic compounds widely distributed in fruits and vegetables. Their consumption has been shown to prevent some chronic diseases. Anthocyanin metabolism, however, is still not fully understood. The aim of this work was to evaluate the bioavailability of anthocyanins in humans consuming a meal containing strawberries and to identify possible metabolites in urine. Six healthy volunteers (three women and three men) consumed a meal containing 200 g strawberries (providing 179 micro mol pelargonidin-3-glucoside). Urine samples were collected before and after the meal and rapidly treated by solid-phase extraction. Identification and quantification of anthocyanin metabolites were carried out by HPLC-ESI-MS-MS and HPLC with UV-visible detection, respectively. In addition to pelargonidin-3-glucoside, five anthocyanin metabolites were identified in urine: three monoglucuronides of pelargonidin, one sulfoconjugate of pelargonidin and pelargonidin itself. Total urinary excretion of strawberry anthocyanin metabolites corresponded to 1.80 +/- 0.29% (mean +/- SEM, n = 6) of pelargonidin-3-glucoside ingested. More than 80% of this excretion was related to a monoglucuronide. Four hours after the meal, more than two-thirds of anthocyanin metabolites had been excreted, although urinary excretion of the metabolites continued until the end of the 24-h experiment. This study demonstrated that anthocyanins were glucuro- and sulfo-conjugated in humans and that the main metabolite of strawberry anthocyanins in human urine was a monoglucuronide of pelargonidin. SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/12730413/Strawberry_anthocyanins_are_recovered_in_urine_as_glucuro__and_sulfoconjugates_in_humans_ DB - PRIME DP - Unbound Medicine ER -