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Cyanobacterial ecotypes in different optical microenvironments of a 68 degrees C hot spring mat community revealed by 16S-23S rRNA internal transcribed spacer region variation.
Appl Environ Microbiol. 2003 May; 69(5):2893-8.AE

Abstract

We examined the population of unicellular cyanobacteria (Synechococcus) in the upper 3-mm vertical interval of a 68 degrees C region of a microbial mat in a hot spring effluent channel (Yellowstone National Park, Wyoming). Fluorescence microscopy and microsensor measurements of O(2) and oxygenic photosynthesis demonstrated the existence of physiologically distinct Synechococcus populations at different depths along a light gradient quantified by scalar irradiance microprobes. Molecular methods were used to evaluate whether physiologically distinct populations could be correlated with genetically distinct populations over the vertical interval. We were unable to identify patterns in genetic variation in Synechococcus 16S rRNA sequences that correlate with different vertically distributed populations. However, patterns of variation at the internal transcribed spacer locus separating 16S and 23S rRNA genes suggested the existence of closely related but genetically distinct populations corresponding to different functional populations occurring at different depths.

Authors+Show Affiliations

Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717-3120, USA. mferris@chnola-research.orgNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12732563

Citation

Ferris, Mike J., et al. "Cyanobacterial Ecotypes in Different Optical Microenvironments of a 68 Degrees C Hot Spring Mat Community Revealed By 16S-23S rRNA Internal Transcribed Spacer Region Variation." Applied and Environmental Microbiology, vol. 69, no. 5, 2003, pp. 2893-8.
Ferris MJ, Kühl M, Wieland A, et al. Cyanobacterial ecotypes in different optical microenvironments of a 68 degrees C hot spring mat community revealed by 16S-23S rRNA internal transcribed spacer region variation. Appl Environ Microbiol. 2003;69(5):2893-8.
Ferris, M. J., Kühl, M., Wieland, A., & Ward, D. M. (2003). Cyanobacterial ecotypes in different optical microenvironments of a 68 degrees C hot spring mat community revealed by 16S-23S rRNA internal transcribed spacer region variation. Applied and Environmental Microbiology, 69(5), 2893-8.
Ferris MJ, et al. Cyanobacterial Ecotypes in Different Optical Microenvironments of a 68 Degrees C Hot Spring Mat Community Revealed By 16S-23S rRNA Internal Transcribed Spacer Region Variation. Appl Environ Microbiol. 2003;69(5):2893-8. PubMed PMID: 12732563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyanobacterial ecotypes in different optical microenvironments of a 68 degrees C hot spring mat community revealed by 16S-23S rRNA internal transcribed spacer region variation. AU - Ferris,Mike J, AU - Kühl,Michael, AU - Wieland,Andrea, AU - Ward,David M, PY - 2003/5/7/pubmed PY - 2003/8/6/medline PY - 2003/5/7/entrez SP - 2893 EP - 8 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 69 IS - 5 N2 - We examined the population of unicellular cyanobacteria (Synechococcus) in the upper 3-mm vertical interval of a 68 degrees C region of a microbial mat in a hot spring effluent channel (Yellowstone National Park, Wyoming). Fluorescence microscopy and microsensor measurements of O(2) and oxygenic photosynthesis demonstrated the existence of physiologically distinct Synechococcus populations at different depths along a light gradient quantified by scalar irradiance microprobes. Molecular methods were used to evaluate whether physiologically distinct populations could be correlated with genetically distinct populations over the vertical interval. We were unable to identify patterns in genetic variation in Synechococcus 16S rRNA sequences that correlate with different vertically distributed populations. However, patterns of variation at the internal transcribed spacer locus separating 16S and 23S rRNA genes suggested the existence of closely related but genetically distinct populations corresponding to different functional populations occurring at different depths. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/12732563/Cyanobacterial_ecotypes_in_different_optical_microenvironments_of_a_68_degrees_C_hot_spring_mat_community_revealed_by_16S_23S_rRNA_internal_transcribed_spacer_region_variation_ L2 - https://journals.asm.org/doi/10.1128/AEM.69.5.2893-2898.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -