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Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae.
Curr Genet. 2003 Jul; 43(4):263-72.CG

Abstract

A central problem in our understanding of mitochondrial (mt) function remains the question of how coordinate transcriptional control is accomplished between nucleus and mitochondria. Here, we report the initial characterization of a protein of previously unknown function, the product of the YMR030 W gene, that appears to mediate such coordinate gene expression. Expression of YMR030 W is glucose-repressible; a deletion mutant for this gene shows a severe growth defect on glycerol-, but not glucose- or ethanol-based medium. In that mutant, transcript levels from GUT1 and GUT2 are highly attenuated compared with those of the wild-type parent when both are grown on glycerol-based medium. Under the same growth conditions, transcripts from the mt OLI1 gene, which has one copy of a mt upstream activating sequence (UAS) in its 5'-flanking region, are attenuated in the DeltaYMR030 W mutant, but mRNA from the mt COX3 (OXI2) gene, which lacks the mt UAS, are not. Some nuclear genes encoding mt-related proteins also show low transcript levels in the DeltaYMR030 W mutant in comparison with those of the wild-type parent strain during glycerol-based growth. Localization of the protein, via its expression fused to green fluorescent protein, indicates that it is present in both nucleus and mitochondria, supporting a respiration-related transcriptional role for this gene product in both cellular genetic compartments. Because of its role in both respiratory growth and mt function, we designate the YMR030 W coding sequence RSF1 (respiration factor 1).

Authors+Show Affiliations

Department of Immunology and Microbiology, Wayne State University School of Medicine, Gordon H. Scott Hall, 540 East Canfield Avenue, Detroit, MI 48201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12734673

Citation

Lu, Lin, et al. "Rsf1p, a Protein Required for Respiratory Growth of Saccharomyces Cerevisiae." Current Genetics, vol. 43, no. 4, 2003, pp. 263-72.
Lu L, Roberts G, Simon K, et al. Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae. Curr Genet. 2003;43(4):263-72.
Lu, L., Roberts, G., Simon, K., Yu, J., & Hudson, A. P. (2003). Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae. Current Genetics, 43(4), 263-72.
Lu L, et al. Rsf1p, a Protein Required for Respiratory Growth of Saccharomyces Cerevisiae. Curr Genet. 2003;43(4):263-72. PubMed PMID: 12734673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae. AU - Lu,Lin, AU - Roberts,George, AU - Simon,Kirk, AU - Yu,Jia, AU - Hudson,Alan P, Y1 - 2003/05/07/ PY - 2003/02/04/received PY - 2003/04/01/revised PY - 2003/04/04/accepted PY - 2003/5/8/pubmed PY - 2004/4/29/medline PY - 2003/5/8/entrez SP - 263 EP - 72 JF - Current genetics JO - Curr Genet VL - 43 IS - 4 N2 - A central problem in our understanding of mitochondrial (mt) function remains the question of how coordinate transcriptional control is accomplished between nucleus and mitochondria. Here, we report the initial characterization of a protein of previously unknown function, the product of the YMR030 W gene, that appears to mediate such coordinate gene expression. Expression of YMR030 W is glucose-repressible; a deletion mutant for this gene shows a severe growth defect on glycerol-, but not glucose- or ethanol-based medium. In that mutant, transcript levels from GUT1 and GUT2 are highly attenuated compared with those of the wild-type parent when both are grown on glycerol-based medium. Under the same growth conditions, transcripts from the mt OLI1 gene, which has one copy of a mt upstream activating sequence (UAS) in its 5'-flanking region, are attenuated in the DeltaYMR030 W mutant, but mRNA from the mt COX3 (OXI2) gene, which lacks the mt UAS, are not. Some nuclear genes encoding mt-related proteins also show low transcript levels in the DeltaYMR030 W mutant in comparison with those of the wild-type parent strain during glycerol-based growth. Localization of the protein, via its expression fused to green fluorescent protein, indicates that it is present in both nucleus and mitochondria, supporting a respiration-related transcriptional role for this gene product in both cellular genetic compartments. Because of its role in both respiratory growth and mt function, we designate the YMR030 W coding sequence RSF1 (respiration factor 1). SN - 0172-8083 UR - https://www.unboundmedicine.com/medline/citation/12734673/Rsf1p_a_protein_required_for_respiratory_growth_of_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -