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RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae.
Nucleic Acids Res. 2003 May 15; 31(10):2524-33.NA

Abstract

A strain of Saccharomyces cerevisiae, defective in small subunit ribosomal RNA processing, has a mutation in YOR145c ORF that converts Gly235 to Asp. Yor145c is a nucleolar protein required for cell viability and has been reported recently to be present in 90S pre-ribosomal particles. The Gly235Asp mutation in YOR145c is found in a KH-type RNA-binding domain and causes a marked deficiency in 18S rRNA production. Detailed studies by northern blotting and primer extension analyses show that the mutant strain impairs the early pre-rRNA processing cleavage essentially at sites A1 and A2, leading to accumulation of a 22S dead-end processing product that is found in only a few rRNA processing mutants. Furthermore, U3, U14, snR10 and snR30 snoRNAs, involved in early pre-rRNA cleavages, are not destabilized by the YOR145c mutation. As the protein encoded by YOR145c is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as RRP20.

Authors+Show Affiliations

Molecular Genetics and Evolution Group, Research School of Biological Sciences, The Australian National University, GPO Box 475, Canberra ACT 2601, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12736301

Citation

Senapin, Saengchan, et al. "RRP20, a Component of the 90S Preribosome, Is Required for pre-18S rRNA Processing in Saccharomyces Cerevisiae." Nucleic Acids Research, vol. 31, no. 10, 2003, pp. 2524-33.
Senapin S, Clark-Walker GD, Chen XJ, et al. RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2003;31(10):2524-33.
Senapin, S., Clark-Walker, G. D., Chen, X. J., Séraphin, B., & Daugeron, M. C. (2003). RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Research, 31(10), 2524-33.
Senapin S, et al. RRP20, a Component of the 90S Preribosome, Is Required for pre-18S rRNA Processing in Saccharomyces Cerevisiae. Nucleic Acids Res. 2003 May 15;31(10):2524-33. PubMed PMID: 12736301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae. AU - Senapin,Saengchan, AU - Clark-Walker,G Desmond, AU - Chen,Xin Jie, AU - Séraphin,Bertrand, AU - Daugeron,Marie-Claire, PY - 2003/5/9/pubmed PY - 2003/6/26/medline PY - 2003/5/9/entrez SP - 2524 EP - 33 JF - Nucleic acids research JO - Nucleic Acids Res VL - 31 IS - 10 N2 - A strain of Saccharomyces cerevisiae, defective in small subunit ribosomal RNA processing, has a mutation in YOR145c ORF that converts Gly235 to Asp. Yor145c is a nucleolar protein required for cell viability and has been reported recently to be present in 90S pre-ribosomal particles. The Gly235Asp mutation in YOR145c is found in a KH-type RNA-binding domain and causes a marked deficiency in 18S rRNA production. Detailed studies by northern blotting and primer extension analyses show that the mutant strain impairs the early pre-rRNA processing cleavage essentially at sites A1 and A2, leading to accumulation of a 22S dead-end processing product that is found in only a few rRNA processing mutants. Furthermore, U3, U14, snR10 and snR30 snoRNAs, involved in early pre-rRNA cleavages, are not destabilized by the YOR145c mutation. As the protein encoded by YOR145c is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as RRP20. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/12736301/RRP20_a_component_of_the_90S_preribosome_is_required_for_pre_18S_rRNA_processing_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkg366 DB - PRIME DP - Unbound Medicine ER -