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Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes.
Nucleic Acids Res. 2003 May 15; 31(10):2576-85.NA

Abstract

Sister chromatid exchange (SCE) can occur by several recombination mechanisms, including those directly initiated by double-strand breaks (DSBs), such as gap repair and break-induced replication (BIR), and those initiated when DNA polymerases stall, such as template switching. To elucidate SCE recombination mechanisms, we determined whether spontaneous and DNA damage-associated SCE requires specific genes within the RAD52 and RAD3 epistasis groups in Saccharomyces cerevisiae strains containing two his3 fragments, his3-Delta5' and his3-Delta3'::HOcs. SCE frequencies were measured after cells were exposed to UV, X-rays, 4-nitroquinoline 1-oxide (4-NQO) and methyl methanesulfonate (MMS), or when an HO endonuclease-induced DSB was introduced at his3-Delta3'::HOcs. Our data indicate that genes involved in gap repair, such as RAD55, RAD57 and RAD54, are required for DNA damage-associated SCE but not for spontaneous SCE. RAD50 and RAD59, genes required for BIR, are required for X-ray-associated SCE but not for SCE stimulated by HO-induced DSBs. In comparison with wild type, rates of spontaneous SCE are 10-fold lower in rad51 rad1 but not in either rad51 rad50 or rad51 rad59 double mutants. We propose that gap repair mechanisms are important in DNA damage-associated recombination, whereas alternative pathways, including a template switch pathway, play a role in spontaneous SCE.

Authors+Show Affiliations

Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208-3479, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12736307

Citation

Dong, Zheng, and Michael Fasullo. "Multiple Recombination Pathways for Sister Chromatid Exchange in Saccharomyces Cerevisiae: Role of RAD1 and the RAD52 Epistasis Group Genes." Nucleic Acids Research, vol. 31, no. 10, 2003, pp. 2576-85.
Dong Z, Fasullo M. Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes. Nucleic Acids Res. 2003;31(10):2576-85.
Dong, Z., & Fasullo, M. (2003). Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes. Nucleic Acids Research, 31(10), 2576-85.
Dong Z, Fasullo M. Multiple Recombination Pathways for Sister Chromatid Exchange in Saccharomyces Cerevisiae: Role of RAD1 and the RAD52 Epistasis Group Genes. Nucleic Acids Res. 2003 May 15;31(10):2576-85. PubMed PMID: 12736307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes. AU - Dong,Zheng, AU - Fasullo,Michael, PY - 2003/5/9/pubmed PY - 2003/6/26/medline PY - 2003/5/9/entrez SP - 2576 EP - 85 JF - Nucleic acids research JO - Nucleic Acids Res VL - 31 IS - 10 N2 - Sister chromatid exchange (SCE) can occur by several recombination mechanisms, including those directly initiated by double-strand breaks (DSBs), such as gap repair and break-induced replication (BIR), and those initiated when DNA polymerases stall, such as template switching. To elucidate SCE recombination mechanisms, we determined whether spontaneous and DNA damage-associated SCE requires specific genes within the RAD52 and RAD3 epistasis groups in Saccharomyces cerevisiae strains containing two his3 fragments, his3-Delta5' and his3-Delta3'::HOcs. SCE frequencies were measured after cells were exposed to UV, X-rays, 4-nitroquinoline 1-oxide (4-NQO) and methyl methanesulfonate (MMS), or when an HO endonuclease-induced DSB was introduced at his3-Delta3'::HOcs. Our data indicate that genes involved in gap repair, such as RAD55, RAD57 and RAD54, are required for DNA damage-associated SCE but not for spontaneous SCE. RAD50 and RAD59, genes required for BIR, are required for X-ray-associated SCE but not for SCE stimulated by HO-induced DSBs. In comparison with wild type, rates of spontaneous SCE are 10-fold lower in rad51 rad1 but not in either rad51 rad50 or rad51 rad59 double mutants. We propose that gap repair mechanisms are important in DNA damage-associated recombination, whereas alternative pathways, including a template switch pathway, play a role in spontaneous SCE. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/12736307/Multiple_recombination_pathways_for_sister_chromatid_exchange_in_Saccharomyces_cerevisiae:_role_of_RAD1_and_the_RAD52_epistasis_group_genes_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkg352 DB - PRIME DP - Unbound Medicine ER -