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Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus.
Clin Diagn Lab Immunol. 2003 May; 10(3):439-42.CD

Abstract

Measles is a highly contagious respiratory virus infection, with typical clinical symptoms including maculopapular rash, fever, cough, coryza, and conjunctivitis. Despite implementation of widespread vaccination programs throughout the world, the rates of global morbidity and mortality are still considerable. This study was performed to design a reliable indirect enzyme-linked immunosorbent assay (ELISA) to measure measles-specific immunoglobulin M (IgM). First, human IgM was purified, and then an anti-IgM antibody was produced in rabbits and purified in a multistep process. The rabbit IgG against human IgM was conjugated with peroxidase. Measles virus-infected Vero cells produced viral antigen. One hundred serum samples from infants of 9 to 18 months of age, mostly vaccinated, were evaluated for determining the presence of specific IgM antibodies against measles virus. The samples were also evaluated for neutralizing antibodies against measles virus by a microneutralization test (MNT). By comparing the results of the ELISA with those of MNT, it was demonstrated that ELISA had a sensitivity and specificity of 100 and 92%, respectively. On the other hand, when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit (EIAgen; Adaltis Italia SPa, Bologna, Italy), a high level of agreement was shown (k = 0.926).

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Authors+Show Affiliations

Roodbari F
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
Roustai MH
No affiliation info available
Mostafaie A
No affiliation info available
Soleimanjdahi H
No affiliation info available
Foroshani RS
No affiliation info available
Sabahi F
No affiliation info available

MeSH

AnimalsAntibodies, Anti-IdiotypicAntibodies, ViralChlorocebus aethiopsEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectHumansImmunoglobulin MInfantIranMeaslesMeasles virusNeutralization TestsRabbitsSensitivity and SpecificityVero Cells

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12738645

Citation

Roodbari, F, et al. "Development of an Enzyme-linked Immunosorbent Assay for Immunoglobulin M Antibodies Against Measles Virus." Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 3, 2003, pp. 439-42.
Roodbari F, Roustai MH, Mostafaie A, et al. Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus. Clin Diagn Lab Immunol. 2003;10(3):439-42.
Roodbari, F., Roustai, M. H., Mostafaie, A., Soleimanjdahi, H., Foroshani, R. S., & Sabahi, F. (2003). Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus. Clinical and Diagnostic Laboratory Immunology, 10(3), 439-42.
Roodbari F, et al. Development of an Enzyme-linked Immunosorbent Assay for Immunoglobulin M Antibodies Against Measles Virus. Clin Diagn Lab Immunol. 2003;10(3):439-42. PubMed PMID: 12738645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus. AU - Roodbari,F, AU - Roustai,M H, AU - Mostafaie,A, AU - Soleimanjdahi,H, AU - Foroshani,R Sarrami, AU - Sabahi,F, PY - 2003/5/10/pubmed PY - 2004/3/17/medline PY - 2003/5/10/entrez SP - 439 EP - 42 JF - Clinical and diagnostic laboratory immunology JO - Clin Diagn Lab Immunol VL - 10 IS - 3 N2 - Measles is a highly contagious respiratory virus infection, with typical clinical symptoms including maculopapular rash, fever, cough, coryza, and conjunctivitis. Despite implementation of widespread vaccination programs throughout the world, the rates of global morbidity and mortality are still considerable. This study was performed to design a reliable indirect enzyme-linked immunosorbent assay (ELISA) to measure measles-specific immunoglobulin M (IgM). First, human IgM was purified, and then an anti-IgM antibody was produced in rabbits and purified in a multistep process. The rabbit IgG against human IgM was conjugated with peroxidase. Measles virus-infected Vero cells produced viral antigen. One hundred serum samples from infants of 9 to 18 months of age, mostly vaccinated, were evaluated for determining the presence of specific IgM antibodies against measles virus. The samples were also evaluated for neutralizing antibodies against measles virus by a microneutralization test (MNT). By comparing the results of the ELISA with those of MNT, it was demonstrated that ELISA had a sensitivity and specificity of 100 and 92%, respectively. On the other hand, when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit (EIAgen; Adaltis Italia SPa, Bologna, Italy), a high level of agreement was shown (k = 0.926). SN - 1071-412X UR - https://www.unboundmedicine.com/medline/citation/12738645/Development_of_an_enzyme_linked_immunosorbent_assay_for_immunoglobulin_M_antibodies_against_measles_virus_ DB - PRIME DP - Unbound Medicine ER -