Tags

Type your tag names separated by a space and hit enter

Microbial transformation of naproxen by Cunninghamella species.
Acta Pharmacol Sin. 2003 May; 24(5):442-7.AP

Abstract

AIM

The metabolites of naproxen produced by Cunninghamella species were isolated and identified, and further to compare the similarities between microbial transformation and mammalian metabolism.

METHODS

Naproxen was transformed by three strains of Cunninghammella species (Cunninghamella blakeslesna AS 3.153, Cunninghamella echinulata AS 3.2004, and Cunninghamella elegans AS 3.156). The metabolites of naproxen were separated and assayed by liquid chromatography-mass spectrometry method. Semi-preparative HPLC was used to isolate the major metabolite, and the structure was identified by nuclear magnetic resonance (NMR) and mass spectrometry.

RESULTS

Naproxen was transformed into 2 metabolites, desmethylnaproxen and desmethylnaproxen-6-O-sulfate, both were the known mammalian metabolites. The conjugated metabolite was newly detected in microbial transformation samples.

CONCLUSION

The microbial transformation of naproxen has some similarities with the metabolism of naproxen in mammals. The fungi belonging to Cunninghamella species could be used as complementary in vitro models for drug metabolism to predict and produce the metabolites of drugs in mammals.

Authors+Show Affiliations

Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China. zhongdf@china.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12740180

Citation

Zhong, Da-Fang, et al. "Microbial Transformation of Naproxen By Cunninghamella Species." Acta Pharmacologica Sinica, vol. 24, no. 5, 2003, pp. 442-7.
Zhong DF, Sun L, Liu L, et al. Microbial transformation of naproxen by Cunninghamella species. Acta Pharmacol Sin. 2003;24(5):442-7.
Zhong, D. F., Sun, L., Liu, L., & Huang, H. H. (2003). Microbial transformation of naproxen by Cunninghamella species. Acta Pharmacologica Sinica, 24(5), 442-7.
Zhong DF, et al. Microbial Transformation of Naproxen By Cunninghamella Species. Acta Pharmacol Sin. 2003;24(5):442-7. PubMed PMID: 12740180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial transformation of naproxen by Cunninghamella species. AU - Zhong,Da-Fang, AU - Sun,Lu, AU - Liu,Lei, AU - Huang,Hai-Hua, PY - 2003/5/13/pubmed PY - 2003/10/8/medline PY - 2003/5/13/entrez SP - 442 EP - 7 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 24 IS - 5 N2 - AIM: The metabolites of naproxen produced by Cunninghamella species were isolated and identified, and further to compare the similarities between microbial transformation and mammalian metabolism. METHODS: Naproxen was transformed by three strains of Cunninghammella species (Cunninghamella blakeslesna AS 3.153, Cunninghamella echinulata AS 3.2004, and Cunninghamella elegans AS 3.156). The metabolites of naproxen were separated and assayed by liquid chromatography-mass spectrometry method. Semi-preparative HPLC was used to isolate the major metabolite, and the structure was identified by nuclear magnetic resonance (NMR) and mass spectrometry. RESULTS: Naproxen was transformed into 2 metabolites, desmethylnaproxen and desmethylnaproxen-6-O-sulfate, both were the known mammalian metabolites. The conjugated metabolite was newly detected in microbial transformation samples. CONCLUSION: The microbial transformation of naproxen has some similarities with the metabolism of naproxen in mammals. The fungi belonging to Cunninghamella species could be used as complementary in vitro models for drug metabolism to predict and produce the metabolites of drugs in mammals. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/12740180/Microbial_transformation_of_naproxen_by_Cunninghamella_species_ L2 - http://www.chinaphar.com/1671-4083/24/442.pdf DB - PRIME DP - Unbound Medicine ER -