Tags

Type your tag names separated by a space and hit enter

Sensitive determination of ephedrine and pseudoephedrine by capillary electrophoresis with laser-induced fluorescence detection.
Analyst. 2003 Apr; 128(4):369-72.A

Abstract

A CE-LIF method was developed for the separation and sensitive detection of ephedrine and pseudoephedrine after derivatization by 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol (NBD-C1). The derivatization and separation conditions were investigated in detail and the optimum conditions were obtained. Under the optimum experiment conditions, good linearity relationships (correlation coefficients: 0.9942 for ephedrine and 0.9970 for pseudoephedrine) between the peak heights and concentrations of the analytes were obtained (0.7-140 microM). The detection limits were 0.16 microM for ephedrine and 0.17 microM for pseudoephedrine, which indicated that the sensitivities were at least ten times improved over those reported in the literature obtained by UV detection. The method was applied to the analysis of ephedrine and pseudoephedrine in ephedra herb plants and preparations with good results.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12741643

Citation

Zhang, Jiyou, et al. "Sensitive Determination of Ephedrine and Pseudoephedrine By Capillary Electrophoresis With Laser-induced Fluorescence Detection." The Analyst, vol. 128, no. 4, 2003, pp. 369-72.
Zhang J, Xie J, Chen X, et al. Sensitive determination of ephedrine and pseudoephedrine by capillary electrophoresis with laser-induced fluorescence detection. Analyst. 2003;128(4):369-72.
Zhang, J., Xie, J., Chen, X., & Hua, Z. (2003). Sensitive determination of ephedrine and pseudoephedrine by capillary electrophoresis with laser-induced fluorescence detection. The Analyst, 128(4), 369-72.
Zhang J, et al. Sensitive Determination of Ephedrine and Pseudoephedrine By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Analyst. 2003;128(4):369-72. PubMed PMID: 12741643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of ephedrine and pseudoephedrine by capillary electrophoresis with laser-induced fluorescence detection. AU - Zhang,Jiyou, AU - Xie,Jianping, AU - Chen,Xingguo, AU - Hua,Zhide, PY - 2003/5/14/pubmed PY - 2003/7/17/medline PY - 2003/5/14/entrez SP - 369 EP - 72 JF - The Analyst JO - Analyst VL - 128 IS - 4 N2 - A CE-LIF method was developed for the separation and sensitive detection of ephedrine and pseudoephedrine after derivatization by 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol (NBD-C1). The derivatization and separation conditions were investigated in detail and the optimum conditions were obtained. Under the optimum experiment conditions, good linearity relationships (correlation coefficients: 0.9942 for ephedrine and 0.9970 for pseudoephedrine) between the peak heights and concentrations of the analytes were obtained (0.7-140 microM). The detection limits were 0.16 microM for ephedrine and 0.17 microM for pseudoephedrine, which indicated that the sensitivities were at least ten times improved over those reported in the literature obtained by UV detection. The method was applied to the analysis of ephedrine and pseudoephedrine in ephedra herb plants and preparations with good results. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/12741643/Sensitive_determination_of_ephedrine_and_pseudoephedrine_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1039/b211862a DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.