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Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids).
J Virol. 2003 Jun; 77(11):6528-32.JV

Abstract

Viroids, small circular RNAs that replicate independently and in most cases incite diseases in plants, are classified into the families Pospiviroidae, composed of species with a central conserved region (CCR) and without hammerhead ribozymes, and Avsunviroidae, composed of three members lacking CCR but able to self-cleave in both polarity strands through hammerhead ribozymes. Here we report the biological and molecular properties of Eggplant latent viroid (ELVd). Purified circular ELVd induces symptomless infections when inoculated into eggplant seedlings. ELVd can be transmitted horizontally and through seed. Sequencing 10 complete cDNA clones showed that ELVd is a circular RNA of 332 to 335 nucleotides with high variability. This RNA can adopt a quasi-rod-like secondary structure of minimal free energy and alternative foldings that permit formation of stable hammerhead structures in plus and minus strands. The ribozymes are active in vitro and, most likely, in vivo. Considering the ELVd properties to be intermediate between those of the two genera of family Avsunviroidae, we propose ELVd as the type species of a third genus with the name ELAVIROID:

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Authors+Show Affiliations

Fadda Z
Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Spain.
Daròs JA
No affiliation info available
Fagoaga C
No affiliation info available
Flores R
No affiliation info available
Duran-Vila N
No affiliation info available

MeSH

Base SequenceMolecular Sequence DataPlant DiseasesRNA, CatalyticRNA, ViralSequence Analysis, DNASolanum melongenaViroids

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12743309

Citation

Fadda, Z, et al. "Eggplant Latent Viroid, the Candidate Type Species for a New Genus Within the Family Avsunviroidae (hammerhead Viroids)." Journal of Virology, vol. 77, no. 11, 2003, pp. 6528-32.
Fadda Z, Daròs JA, Fagoaga C, et al. Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). J Virol. 2003;77(11):6528-32.
Fadda, Z., Daròs, J. A., Fagoaga, C., Flores, R., & Duran-Vila, N. (2003). Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). Journal of Virology, 77(11), 6528-32.
Fadda Z, et al. Eggplant Latent Viroid, the Candidate Type Species for a New Genus Within the Family Avsunviroidae (hammerhead Viroids). J Virol. 2003;77(11):6528-32. PubMed PMID: 12743309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). AU - Fadda,Z, AU - Daròs,J A, AU - Fagoaga,C, AU - Flores,R, AU - Duran-Vila,N, PY - 2003/5/14/pubmed PY - 2003/6/13/medline PY - 2003/5/14/entrez SP - 6528 EP - 32 JF - Journal of virology JO - J Virol VL - 77 IS - 11 N2 - Viroids, small circular RNAs that replicate independently and in most cases incite diseases in plants, are classified into the families Pospiviroidae, composed of species with a central conserved region (CCR) and without hammerhead ribozymes, and Avsunviroidae, composed of three members lacking CCR but able to self-cleave in both polarity strands through hammerhead ribozymes. Here we report the biological and molecular properties of Eggplant latent viroid (ELVd). Purified circular ELVd induces symptomless infections when inoculated into eggplant seedlings. ELVd can be transmitted horizontally and through seed. Sequencing 10 complete cDNA clones showed that ELVd is a circular RNA of 332 to 335 nucleotides with high variability. This RNA can adopt a quasi-rod-like secondary structure of minimal free energy and alternative foldings that permit formation of stable hammerhead structures in plus and minus strands. The ribozymes are active in vitro and, most likely, in vivo. Considering the ELVd properties to be intermediate between those of the two genera of family Avsunviroidae, we propose ELVd as the type species of a third genus with the name ELAVIROID: SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/12743309/Eggplant_latent_viroid_the_candidate_type_species_for_a_new_genus_within_the_family_Avsunviroidae__hammerhead_viroids__ DB - PRIME DP - Unbound Medicine ER -