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Identification of cross-reactive and genuine Parietaria judaica pollen allergens.
J Allergy Clin Immunol. 2003 May; 111(5):974-9.JA

Abstract

BACKGROUND

The weed Parietaria judaica is one of the most important pollen allergen sources in the Mediterranean area.

OBJECTIVE

We sought to identify P judaica pollen allergen, which might be used to serologically distinguish genuine Parietaria sensitization and cross-reactivity to allergens from other weed species (eg, mugwort and ragweed).

METHODS

The allergen profile of P judaica IgE-reactive sera from weed pollen-sensitized allergic individuals from the Mediterranean region (n = 36) with high Parietaria pollen exposure and from weed pollen-allergic patients with little or no Parietaria exposure (Austria, n = 42; Scandinavia, n = 8; United States, n = 19) was established by CAP FEIA measurements and by IgE immunoblot inhibition experiments with recombinant allergens.

RESULTS

The majority (83%) of the Mediterranean weed pollen-allergic patients mounted high IgE antibody levels (mean specific IgE, 20.89 kUA/L) against recombinant (r) Par j 2, whereas only 7% of the non-Mediterranean weed-allergic patients showed low IgE reactivity to rPar j 2 (mean specific IgE, 1.03 kUA/L). The cytoskeletal protein profilin and a 2-EF-hand calcium-binding allergen were identified as cross-reactive Parietaria allergens, which were recognized preferentially by Parietaria -positive, non-Mediterranean weed pollen-allergic patients.

CONCLUSION

rPar j 2 might be used as a diagnostic marker allergen to identify weed pollen-allergic patients who are genuinely sensitized against Parietaria pollen and thus would be particularly suited for specific immunotherapy with Parietaria pollen extract.

Authors+Show Affiliations

Department of Pathophysiology, AKH, University of Vienna, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12743560

Citation

Stumvoll, Sabine, et al. "Identification of Cross-reactive and Genuine Parietaria Judaica Pollen Allergens." The Journal of Allergy and Clinical Immunology, vol. 111, no. 5, 2003, pp. 974-9.
Stumvoll S, Westritschnig K, Lidholm J, et al. Identification of cross-reactive and genuine Parietaria judaica pollen allergens. J Allergy Clin Immunol. 2003;111(5):974-9.
Stumvoll, S., Westritschnig, K., Lidholm, J., Spitzauer, S., Colombo, P., Duro, G., Kraft, D., Geraci, D., & Valenta, R. (2003). Identification of cross-reactive and genuine Parietaria judaica pollen allergens. The Journal of Allergy and Clinical Immunology, 111(5), 974-9.
Stumvoll S, et al. Identification of Cross-reactive and Genuine Parietaria Judaica Pollen Allergens. J Allergy Clin Immunol. 2003;111(5):974-9. PubMed PMID: 12743560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cross-reactive and genuine Parietaria judaica pollen allergens. AU - Stumvoll,Sabine, AU - Westritschnig,Kerstin, AU - Lidholm,Jonas, AU - Spitzauer,Susanne, AU - Colombo,Paolo, AU - Duro,Giovanni, AU - Kraft,Dietrich, AU - Geraci,Domenico, AU - Valenta,Rudolf, PY - 2003/5/14/pubmed PY - 2003/6/21/medline PY - 2003/5/14/entrez SP - 974 EP - 9 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 111 IS - 5 N2 - BACKGROUND: The weed Parietaria judaica is one of the most important pollen allergen sources in the Mediterranean area. OBJECTIVE: We sought to identify P judaica pollen allergen, which might be used to serologically distinguish genuine Parietaria sensitization and cross-reactivity to allergens from other weed species (eg, mugwort and ragweed). METHODS: The allergen profile of P judaica IgE-reactive sera from weed pollen-sensitized allergic individuals from the Mediterranean region (n = 36) with high Parietaria pollen exposure and from weed pollen-allergic patients with little or no Parietaria exposure (Austria, n = 42; Scandinavia, n = 8; United States, n = 19) was established by CAP FEIA measurements and by IgE immunoblot inhibition experiments with recombinant allergens. RESULTS: The majority (83%) of the Mediterranean weed pollen-allergic patients mounted high IgE antibody levels (mean specific IgE, 20.89 kUA/L) against recombinant (r) Par j 2, whereas only 7% of the non-Mediterranean weed-allergic patients showed low IgE reactivity to rPar j 2 (mean specific IgE, 1.03 kUA/L). The cytoskeletal protein profilin and a 2-EF-hand calcium-binding allergen were identified as cross-reactive Parietaria allergens, which were recognized preferentially by Parietaria -positive, non-Mediterranean weed pollen-allergic patients. CONCLUSION: rPar j 2 might be used as a diagnostic marker allergen to identify weed pollen-allergic patients who are genuinely sensitized against Parietaria pollen and thus would be particularly suited for specific immunotherapy with Parietaria pollen extract. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/12743560/Identification_of_cross_reactive_and_genuine_Parietaria_judaica_pollen_allergens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674903007413 DB - PRIME DP - Unbound Medicine ER -