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Peh production, flagellum synthesis, and virulence reduced in Erwinia carotovora subsp. carotovora by mutation in a homologue of cytR.
Mol Plant Microbe Interact. 2003 May; 16(5):389-97.MP

Abstract

Erwinia carotovora subsp. carotovora is a causal agent of soft-rot diseases in a wide variety of plants. Here, we have isolated a new regulatory factor involved in the virulence of E. carotovora subsp. carotovora by in vivo insertional mutagenesis using a transposon Tn5. The gene was homologous to cytR encoding a transcriptional repressor of nucleoside uptake and catabolism genes in Escherichia coli, Salmonella typhimurium, and Vibrio cholerae. Phenotypic characterization of a nonpolar deletion mutant of the cytR homologue (delta cytR) revealed that the delta cytR mutant produced a reduced level of polygalacturonase (Peh) and lost its motility compared to that in the parental strain. With electron microscopy, the delta cytR mutant was shown to be aflagellate. Furthermore, the expression of fliA and fliC (encoding sigma28 and flagellin, respectively) was also reduced in delta cytR mutant. The virulence of delta cytR mutant was reduced in Chinese cabbage and potato compared to that of the parental strain. These results suggest that the CytR homologue of E. carotovora subsp. carotovora positively controls Peh production and flagellum synthesis and plays an important role in its pathogenicity.

Authors+Show Affiliations

Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12744509

Citation

Matsumoto, Hiroyuki, et al. "Peh Production, Flagellum Synthesis, and Virulence Reduced in Erwinia Carotovora Subsp. Carotovora By Mutation in a Homologue of CytR." Molecular Plant-microbe Interactions : MPMI, vol. 16, no. 5, 2003, pp. 389-97.
Matsumoto H, Muroi H, Umehara M, et al. Peh production, flagellum synthesis, and virulence reduced in Erwinia carotovora subsp. carotovora by mutation in a homologue of cytR. Mol Plant Microbe Interact. 2003;16(5):389-97.
Matsumoto, H., Muroi, H., Umehara, M., Yoshitake, Y., & Tsuyumu, S. (2003). Peh production, flagellum synthesis, and virulence reduced in Erwinia carotovora subsp. carotovora by mutation in a homologue of cytR. Molecular Plant-microbe Interactions : MPMI, 16(5), 389-97.
Matsumoto H, et al. Peh Production, Flagellum Synthesis, and Virulence Reduced in Erwinia Carotovora Subsp. Carotovora By Mutation in a Homologue of CytR. Mol Plant Microbe Interact. 2003;16(5):389-97. PubMed PMID: 12744509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peh production, flagellum synthesis, and virulence reduced in Erwinia carotovora subsp. carotovora by mutation in a homologue of cytR. AU - Matsumoto,Hiroyuki, AU - Muroi,Hironobu, AU - Umehara,Masahiro, AU - Yoshitake,Yoshimasa, AU - Tsuyumu,Shinji, PY - 2003/5/15/pubmed PY - 2003/9/17/medline PY - 2003/5/15/entrez SP - 389 EP - 97 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 16 IS - 5 N2 - Erwinia carotovora subsp. carotovora is a causal agent of soft-rot diseases in a wide variety of plants. Here, we have isolated a new regulatory factor involved in the virulence of E. carotovora subsp. carotovora by in vivo insertional mutagenesis using a transposon Tn5. The gene was homologous to cytR encoding a transcriptional repressor of nucleoside uptake and catabolism genes in Escherichia coli, Salmonella typhimurium, and Vibrio cholerae. Phenotypic characterization of a nonpolar deletion mutant of the cytR homologue (delta cytR) revealed that the delta cytR mutant produced a reduced level of polygalacturonase (Peh) and lost its motility compared to that in the parental strain. With electron microscopy, the delta cytR mutant was shown to be aflagellate. Furthermore, the expression of fliA and fliC (encoding sigma28 and flagellin, respectively) was also reduced in delta cytR mutant. The virulence of delta cytR mutant was reduced in Chinese cabbage and potato compared to that of the parental strain. These results suggest that the CytR homologue of E. carotovora subsp. carotovora positively controls Peh production and flagellum synthesis and plays an important role in its pathogenicity. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/12744509/Peh_production_flagellum_synthesis_and_virulence_reduced_in_Erwinia_carotovora_subsp__carotovora_by_mutation_in_a_homologue_of_cytR_ L2 - https://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2003.16.5.389?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -