TY - JOUR T1 - Protection of chickens against highly lethal H5N1 and H7N1 avian influenza viruses with a recombinant fowlpox virus co-expressing H5 haemagglutinin and N1 neuraminidase genes. AU - Qiao,Chuan-Ling, AU - Yu,Kang-Zhen, AU - Jiang,Yong-Ping, AU - Jia,Yong-Qing, AU - Tian,Guo-Bin, AU - Liu,Ming, AU - Deng,Guo-Hua, AU - Wang,Xiu-Rong, AU - Meng,Qing-Wen, AU - Tang,Xiu-Ying, PY - 2003/5/15/pubmed PY - 2003/9/25/medline PY - 2003/5/15/entrez SP - 25 EP - 32 JF - Avian pathology : journal of the W.V.P.A JO - Avian Pathol VL - 32 IS - 1 N2 - Inactivated whole avian influenza virus (AIV) vaccine provides protection against homologous haemagglutinin (HA) subtype virus, but poor protection against a heterologous HA virus. Moreover, it induces chickens to produce antibodies to cross-reactive antigens, especially nucleoprotein, which is limits AIV serological surveillance. In this study, a recombinant fowlpox virus co-expressing HA (H5 subtype) and NA (NI subtype)genes of AIV was evaluated for its ability to protect chickens against intramuscular challenge with a lethal dose of highly pathogenic (HP) AIV. Susceptible chickens were also vaccinated by wing-web puncture with the parent fowlpox vaccine virus. Following challenge 4 weeks later with HPAIV, all chickens vaccinated with recombinant virus were protected, while the chickens vaccinated with either the unaltered parent fowlpox vaccine virus or unvaccinated controls experienced 100% mortality following challenge. This protection was accompanied by the high levels of specific antibody to the respective components of the recombinant vaccine. The above results showed that rFPV-HA-NA could be a potential vaccine to replace current inactivated vaccines for preventing AI. SN - 0307-9457 UR - https://www.unboundmedicine.com/medline/citation/12745375/Protection_of_chickens_against_highly_lethal_H5N1_and_H7N1_avian_influenza_viruses_with_a_recombinant_fowlpox_virus_co_expressing_H5_haemagglutinin_and_N1_neuraminidase_genes_ DB - PRIME DP - Unbound Medicine ER -