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Predicting urine culture results by dipstick testing and phase contrast microscopy.
Pathology. 2003 Apr; 35(2):161-5.P

Abstract

AIMS

Urine is the most frequently received clinical specimen for bacterial culture. To determine whether dipstick or microscopy results reliably predicted the presence or absence of a reportable urinary pathogen we performed dipstick testing and phase contrast microscopy on unspun urine from 500 specimens. We also investigated the relationship between the presence of squamous epithelial cells (SECs) and mixed growth on culture.

METHODS

For each specimen, the presence of leukocyte esterase and urinary nitrite was recorded as well as the quantity of leukocytes (WBC), SECs, and erythrocytes. All cultures had the number and type of each organism recorded. Pyuria was defined as > or =10 WBC/mm(3).

RESULTS

Specimens with <10 SEC/mm(3) had fewer isolates (0.9 isolate per culture) than specimens with > or =10 SEC/mm(3) (two isolates per culture); therefore, SEC contamination was defined as > or =10 SEC/mm(3). Of 500 specimens, 266 (53%) had no growth and 136 (27%) had mixed growth. A total of 288 (58%) specimens had negative dipstick results and completely normal microscopy. Of these, 11 (4%) had a pure growth of a urinary pathogen but the pathogen was present in only three (1%) at > or =10(5) CFU/mL. Of 413 urine specimens without SEC contamination, 323 (78%) had either no detectable growth or pure growth compared with only 41 of 87 (47%) with SEC contamination (P<0.001). Of the 413 urine specimens without SEC contamination, 90 (22%) had > or =2 organisms compared with 46 of 87 (53%) with contamination (P<0.01). Pyuria was present in only 21 of 266 (8%) urine specimens without growth but was present in 60 of 95 (64%) specimens containing a reportable pathogen (P<0.01).

CONCLUSIONS

SEC contamination does not reliably predict cultures with mixed growth, urine specimens with negative dipstick results and microscopy rarely contain a reportable urinary pathogen, and screening algorithms are warranted and justified.

Authors+Show Affiliations

Clinical Microbiology Laboratory, Medical Center, Duke University School of Medicine, Durham, NC, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

12745465

Citation

Smith, Peter, et al. "Predicting Urine Culture Results By Dipstick Testing and Phase Contrast Microscopy." Pathology, vol. 35, no. 2, 2003, pp. 161-5.
Smith P, Morris A, Reller LB. Predicting urine culture results by dipstick testing and phase contrast microscopy. Pathology. 2003;35(2):161-5.
Smith, P., Morris, A., & Reller, L. B. (2003). Predicting urine culture results by dipstick testing and phase contrast microscopy. Pathology, 35(2), 161-5.
Smith P, Morris A, Reller LB. Predicting Urine Culture Results By Dipstick Testing and Phase Contrast Microscopy. Pathology. 2003;35(2):161-5. PubMed PMID: 12745465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Predicting urine culture results by dipstick testing and phase contrast microscopy. AU - Smith,Peter, AU - Morris,Arthur, AU - Reller,L Barth, PY - 2003/5/15/pubmed PY - 2003/7/30/medline PY - 2003/5/15/entrez SP - 161 EP - 5 JF - Pathology JO - Pathology VL - 35 IS - 2 N2 - AIMS: Urine is the most frequently received clinical specimen for bacterial culture. To determine whether dipstick or microscopy results reliably predicted the presence or absence of a reportable urinary pathogen we performed dipstick testing and phase contrast microscopy on unspun urine from 500 specimens. We also investigated the relationship between the presence of squamous epithelial cells (SECs) and mixed growth on culture. METHODS: For each specimen, the presence of leukocyte esterase and urinary nitrite was recorded as well as the quantity of leukocytes (WBC), SECs, and erythrocytes. All cultures had the number and type of each organism recorded. Pyuria was defined as > or =10 WBC/mm(3). RESULTS: Specimens with <10 SEC/mm(3) had fewer isolates (0.9 isolate per culture) than specimens with > or =10 SEC/mm(3) (two isolates per culture); therefore, SEC contamination was defined as > or =10 SEC/mm(3). Of 500 specimens, 266 (53%) had no growth and 136 (27%) had mixed growth. A total of 288 (58%) specimens had negative dipstick results and completely normal microscopy. Of these, 11 (4%) had a pure growth of a urinary pathogen but the pathogen was present in only three (1%) at > or =10(5) CFU/mL. Of 413 urine specimens without SEC contamination, 323 (78%) had either no detectable growth or pure growth compared with only 41 of 87 (47%) with SEC contamination (P<0.001). Of the 413 urine specimens without SEC contamination, 90 (22%) had > or =2 organisms compared with 46 of 87 (53%) with contamination (P<0.01). Pyuria was present in only 21 of 266 (8%) urine specimens without growth but was present in 60 of 95 (64%) specimens containing a reportable pathogen (P<0.01). CONCLUSIONS: SEC contamination does not reliably predict cultures with mixed growth, urine specimens with negative dipstick results and microscopy rarely contain a reportable urinary pathogen, and screening algorithms are warranted and justified. SN - 0031-3025 UR - https://www.unboundmedicine.com/medline/citation/12745465/Predicting_urine_culture_results_by_dipstick_testing_and_phase_contrast_microscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/1FJTTYRLRB9G0C5Q DB - PRIME DP - Unbound Medicine ER -