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Homocysteine, methylenetetrahydrofolate reductase C677T polymorphism and the B-vitamins: a facet of nature-nurture interplay.
Clin Chem Lab Med. 2003 Apr; 41(4):547-53.CC

Abstract

BACKGROUND

Methylenetetrahydrofolate reductase 677 (MTHFR 677) polymorphism may provoke hyperhomocysteinemia when folate status is low. The influence of MTHFR 677 mutation on homocysteine (HCY) levels in relation to vitamin B12 and folate status was investigated in the current study.

SUBJECTS AND METHODS

113 vegetarians, 123 omnivorous Germans, and 117 omnivorous Syrians were recruited. MTHFR 677 genotype, HCY, methylmalonic acid (MMA), total serum vitamin B12, serum folate, and vitamin B6 were determined using conventional methods.

RESULTS

Omnivorous Germans displayed the lowest HCY levels compared with vegetarians and Syrians (median 8.0, 10.4, and 11.3 micromol/l, respectively). The highest serum folate and the highest MMA levels were found in vegetarians (median folate = 30.0; MMA = 355 nmol/l). Among vegetarians and Syrians, TT subjects had higher HCY levels than other genotypes which were, however, no longer significant in the highest folate tertiles. When the data were pooled, the odds ratio (OR, 95% CI) for HCY > 12 micromol/l was 3.81 (1.55-9.34) in TT compared with CC subjects. The OR increased to 28.85 (4.63-179.62) in TT subjects who had folate in the lowest tertile, and to 21.84 (4.81-99.1) in TT subjects who had MMA in the highest MMA tertile.

CONCLUSION

MTHFR 677 TT individuals are more liable to hyperhomocysteinemia under vitamin B12 deficiency than the other two genotypes. In such a case, relative folate shortage may progressively increase HCY levels. TT individuals may have increased folate and vitamin B12 requirements compared to the other CC and CT genotypes.

Authors+Show Affiliations

Herrmann W
Department of Clinical Chemistry, Saarland Medical School, Homburg, Germany. kchwher@uniklinik-saarland.de
Obeid R
No affiliation info available
Schorr H
No affiliation info available
Zarzour W
No affiliation info available
Geisel J
No affiliation info available

MeSH

AdolescentAdultAgedAged, 80 and overFemaleFolic AcidHomocysteineHumansHyperhomocysteinemiaMaleMethylenetetrahydrofolate Reductase (NADPH2)Methylmalonic AcidMiddle AgedOdds RatioOxidoreductases Acting on CH-NH Group DonorsPolymorphism, GeneticVitamin B 12Vitamin B 6

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12747601

Citation

Herrmann, Wolfgang, et al. "Homocysteine, Methylenetetrahydrofolate Reductase C677T Polymorphism and the B-vitamins: a Facet of Nature-nurture Interplay." Clinical Chemistry and Laboratory Medicine, vol. 41, no. 4, 2003, pp. 547-53.
Herrmann W, Obeid R, Schorr H, et al. Homocysteine, methylenetetrahydrofolate reductase C677T polymorphism and the B-vitamins: a facet of nature-nurture interplay. Clin Chem Lab Med. 2003;41(4):547-53.
Herrmann, W., Obeid, R., Schorr, H., Zarzour, W., & Geisel, J. (2003). Homocysteine, methylenetetrahydrofolate reductase C677T polymorphism and the B-vitamins: a facet of nature-nurture interplay. Clinical Chemistry and Laboratory Medicine, 41(4), 547-53.
Herrmann W, et al. Homocysteine, Methylenetetrahydrofolate Reductase C677T Polymorphism and the B-vitamins: a Facet of Nature-nurture Interplay. Clin Chem Lab Med. 2003;41(4):547-53. PubMed PMID: 12747601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Homocysteine, methylenetetrahydrofolate reductase C677T polymorphism and the B-vitamins: a facet of nature-nurture interplay. AU - Herrmann,Wolfgang, AU - Obeid,Rima, AU - Schorr,Heike, AU - Zarzour,Wafika, AU - Geisel,Jürgen, PY - 2003/5/16/pubmed PY - 2003/6/26/medline PY - 2003/5/16/entrez SP - 547 EP - 53 JF - Clinical chemistry and laboratory medicine JO - Clin Chem Lab Med VL - 41 IS - 4 N2 - BACKGROUND: Methylenetetrahydrofolate reductase 677 (MTHFR 677) polymorphism may provoke hyperhomocysteinemia when folate status is low. The influence of MTHFR 677 mutation on homocysteine (HCY) levels in relation to vitamin B12 and folate status was investigated in the current study. SUBJECTS AND METHODS: 113 vegetarians, 123 omnivorous Germans, and 117 omnivorous Syrians were recruited. MTHFR 677 genotype, HCY, methylmalonic acid (MMA), total serum vitamin B12, serum folate, and vitamin B6 were determined using conventional methods. RESULTS: Omnivorous Germans displayed the lowest HCY levels compared with vegetarians and Syrians (median 8.0, 10.4, and 11.3 micromol/l, respectively). The highest serum folate and the highest MMA levels were found in vegetarians (median folate = 30.0; MMA = 355 nmol/l). Among vegetarians and Syrians, TT subjects had higher HCY levels than other genotypes which were, however, no longer significant in the highest folate tertiles. When the data were pooled, the odds ratio (OR, 95% CI) for HCY > 12 micromol/l was 3.81 (1.55-9.34) in TT compared with CC subjects. The OR increased to 28.85 (4.63-179.62) in TT subjects who had folate in the lowest tertile, and to 21.84 (4.81-99.1) in TT subjects who had MMA in the highest MMA tertile. CONCLUSION: MTHFR 677 TT individuals are more liable to hyperhomocysteinemia under vitamin B12 deficiency than the other two genotypes. In such a case, relative folate shortage may progressively increase HCY levels. TT individuals may have increased folate and vitamin B12 requirements compared to the other CC and CT genotypes. SN - 1434-6621 UR - https://www.unboundmedicine.com/medline/citation/12747601/Homocysteine_methylenetetrahydrofolate_reductase_C677T_polymorphism_and_the_B_vitamins:_a_facet_of_nature_nurture_interplay_ DB - PRIME DP - Unbound Medicine ER -