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Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii.
FEMS Yeast Res 2003; 3(4):433-40FY

Abstract

In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. Trehalose synthesis in yeast is mediated by a complex of trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2). In this study, genes encoding TPS1 and TPS2 were isolated from Zygosaccharomyces rouxii (designated ZrTPS1 and ZrTPS2, respectively). They were functionally identified by their complementation of the tps1 and tps2 yeast deletion mutants, which are unable to grow on glucose medium and with heat, respectively. Full-length ZrTPS1 cDNA is composed of 1476 nucleotides encoding a protein of 492 amino acids with a molecular mass of 56 kDa. ZrTPS2 cDNA consists of 2843 nucleotides with an open reading frame of 2700 bp, which encodes a polypeptide of 900 amino acids with a molecular mass of 104 kDa. The amino acid sequence encoded by ZrTPS1 has relatively high homology with TPS1 of Saccharomyces cerevisiae and Schizosaccharomyces pombe, compared with TPS2. Western blot analysis showed that the antibody against S. cerevisiae TPS1 recognizes ZrTPS1. Under normal growth conditions, ZrTPS1 and ZrTPS2 were highly and constitutively expressed, unlike S. cerevisiae TPS1 and TPS2. Salt stress and heat stress reduced the expression of the ZrTPS1 and ZrTPS2 genes, respectively.

Authors+Show Affiliations

Division of Applied Biological Sciences, Sunmoon University, 336-708, Asan, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12748054

Citation

Kwon, Hawk-Bin, et al. "Cloning and Characterization of Genes Encoding Trehalose-6-phosphate Synthase (TPS1) and Trehalose-6-phosphate Phosphatase (TPS2) From Zygosaccharomyces Rouxii." FEMS Yeast Research, vol. 3, no. 4, 2003, pp. 433-40.
Kwon HB, Yeo ET, Hahn SE, et al. Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii. FEMS Yeast Res. 2003;3(4):433-40.
Kwon, H. B., Yeo, E. T., Hahn, S. E., Bae, S. C., Kim, D. Y., & Byun, M. O. (2003). Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii. FEMS Yeast Research, 3(4), pp. 433-40.
Kwon HB, et al. Cloning and Characterization of Genes Encoding Trehalose-6-phosphate Synthase (TPS1) and Trehalose-6-phosphate Phosphatase (TPS2) From Zygosaccharomyces Rouxii. FEMS Yeast Res. 2003;3(4):433-40. PubMed PMID: 12748054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii. AU - Kwon,Hawk-Bin, AU - Yeo,Eun-Taeg, AU - Hahn,Sang-Eun, AU - Bae,Shin-Chul, AU - Kim,Dool-Yi, AU - Byun,Myung-Ok, PY - 2003/5/16/pubmed PY - 2003/9/4/medline PY - 2003/5/16/entrez SP - 433 EP - 40 JF - FEMS yeast research JO - FEMS Yeast Res. VL - 3 IS - 4 N2 - In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. Trehalose synthesis in yeast is mediated by a complex of trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2). In this study, genes encoding TPS1 and TPS2 were isolated from Zygosaccharomyces rouxii (designated ZrTPS1 and ZrTPS2, respectively). They were functionally identified by their complementation of the tps1 and tps2 yeast deletion mutants, which are unable to grow on glucose medium and with heat, respectively. Full-length ZrTPS1 cDNA is composed of 1476 nucleotides encoding a protein of 492 amino acids with a molecular mass of 56 kDa. ZrTPS2 cDNA consists of 2843 nucleotides with an open reading frame of 2700 bp, which encodes a polypeptide of 900 amino acids with a molecular mass of 104 kDa. The amino acid sequence encoded by ZrTPS1 has relatively high homology with TPS1 of Saccharomyces cerevisiae and Schizosaccharomyces pombe, compared with TPS2. Western blot analysis showed that the antibody against S. cerevisiae TPS1 recognizes ZrTPS1. Under normal growth conditions, ZrTPS1 and ZrTPS2 were highly and constitutively expressed, unlike S. cerevisiae TPS1 and TPS2. Salt stress and heat stress reduced the expression of the ZrTPS1 and ZrTPS2 genes, respectively. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/12748054/Cloning_and_characterization_of_genes_encoding_trehalose_6_phosphate_synthase__TPS1__and_trehalose_6_phosphate_phosphatase__TPS2__from_Zygosaccharomyces_rouxii_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1016/S1567-1356(03)00035-7 DB - PRIME DP - Unbound Medicine ER -