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Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm.
Theor Appl Genet. 2003 Sep; 107(5):823-30.TA

Abstract

Seed oil of current zero erucic-acid germplasm of Ethiopian mustard (Brassica carinata A. Braun) is characterized by a low concentration of oleic acid and high concentrations of linoleic and linolenic acids. Sources of increased oleic-acid (HO) and reduced linolenic-acid (LL) concentration have been developed separately in high erucic-acid germplasm. The objectives of the present research were to study the inheritance of the HO and LL traits in crosses HO x LL, and to develop HOLL recombinants, both in high erucic-acid and zero erucic-acid backgrounds. The HO mutant N2-3591 (about 20% oleic acid compared to 9% in conventional high erucic-acid materials), was reciprocally crossed with the LL lines N2-4961 and HF-186 (both with about 5% linolenic acid compared to 12% in standard high erucic-acid materials). Increased oleic acid concentration of N2-3591 was found to be controlled by alleles at one locus (Ol), whereas three different loci for reduced linolenic-acid concentration (Ln, Ln1 and Ln2) were identified in N2-4961 and HF-186. Crosses between N2-3591 and N2-4961 generated HOLL recombinants where levels of increased oleic-acid and reduced linolenic-acid were similar to those of the parents. However, a transgressive segregation for oleic acid was observed in crosses between N2-3591 and HF-186, where F(2) seeds with up to 29.7% oleic acid were obtained, in comparison to an upper limit of 25.1% in the N2-3591 parent grown in the same environment. The transgressive increased oleic-acid was expressed in the F(3) generation and was attributed to the presence of a second locus, designated Ol2. The transgressive trait was transferred to the zero erucic-acid line 25X-1, resulting in a zero erucic-acid germplasm with very high oleic-acid concentration (83.9% compared to 32.9% in 25X-1) and low linolenic-acid concentration (5.0% compared to 16% in 25X-1). Additionally, two other lines exhibiting different stable levels of increased oleic-acid (70.7% and 79.5%, respectively) and reduced levels of linolenic-acid (7.5% and 8.7%, respectively) were isolated.

Authors+Show Affiliations

Velasco L
Instituto de Agricultura Sostenible (CSIC), Apartado 4084, E-14080 Córdoba, Spain. ia2veval@uco.es
Nabloussi A
No affiliation info available
De Haro A
No affiliation info available
Fernández-Martínez JM
No affiliation info available

MeSH

BrassicaBreedingCrosses, GeneticErucic AcidsEthiopiaLinoleic AcidsOleic AcidPlant OilsRecombination, GeneticSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12756471

Citation

Velasco, L, et al. "Development of High-oleic, Low-linolenic Acid Ethiopian-mustard (Brassica Carinata) Germplasm." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 107, no. 5, 2003, pp. 823-30.
Velasco L, Nabloussi A, De Haro A, et al. Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm. Theor Appl Genet. 2003;107(5):823-30.
Velasco, L., Nabloussi, A., De Haro, A., & Fernández-Martínez, J. M. (2003). Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 107(5), 823-30.
Velasco L, et al. Development of High-oleic, Low-linolenic Acid Ethiopian-mustard (Brassica Carinata) Germplasm. Theor Appl Genet. 2003;107(5):823-30. PubMed PMID: 12756471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm. AU - Velasco,L, AU - Nabloussi,A, AU - De Haro,A, AU - Fernández-Martínez,J M, Y1 - 2003/05/17/ PY - 2002/12/12/received PY - 2003/03/25/accepted PY - 2003/5/21/pubmed PY - 2004/1/6/medline PY - 2003/5/21/entrez SP - 823 EP - 30 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 107 IS - 5 N2 - Seed oil of current zero erucic-acid germplasm of Ethiopian mustard (Brassica carinata A. Braun) is characterized by a low concentration of oleic acid and high concentrations of linoleic and linolenic acids. Sources of increased oleic-acid (HO) and reduced linolenic-acid (LL) concentration have been developed separately in high erucic-acid germplasm. The objectives of the present research were to study the inheritance of the HO and LL traits in crosses HO x LL, and to develop HOLL recombinants, both in high erucic-acid and zero erucic-acid backgrounds. The HO mutant N2-3591 (about 20% oleic acid compared to 9% in conventional high erucic-acid materials), was reciprocally crossed with the LL lines N2-4961 and HF-186 (both with about 5% linolenic acid compared to 12% in standard high erucic-acid materials). Increased oleic acid concentration of N2-3591 was found to be controlled by alleles at one locus (Ol), whereas three different loci for reduced linolenic-acid concentration (Ln, Ln1 and Ln2) were identified in N2-4961 and HF-186. Crosses between N2-3591 and N2-4961 generated HOLL recombinants where levels of increased oleic-acid and reduced linolenic-acid were similar to those of the parents. However, a transgressive segregation for oleic acid was observed in crosses between N2-3591 and HF-186, where F(2) seeds with up to 29.7% oleic acid were obtained, in comparison to an upper limit of 25.1% in the N2-3591 parent grown in the same environment. The transgressive increased oleic-acid was expressed in the F(3) generation and was attributed to the presence of a second locus, designated Ol2. The transgressive trait was transferred to the zero erucic-acid line 25X-1, resulting in a zero erucic-acid germplasm with very high oleic-acid concentration (83.9% compared to 32.9% in 25X-1) and low linolenic-acid concentration (5.0% compared to 16% in 25X-1). Additionally, two other lines exhibiting different stable levels of increased oleic-acid (70.7% and 79.5%, respectively) and reduced levels of linolenic-acid (7.5% and 8.7%, respectively) were isolated. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/12756471/Development_of_high_oleic_low_linolenic_acid_Ethiopian_mustard__Brassica_carinata__germplasm_ DB - PRIME DP - Unbound Medicine ER -