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Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus.
FEMS Microbiol Lett. 2003 May 16; 222(1):107-13.FM

Abstract

The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the murB gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity.

Authors+Show Affiliations

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12757953

Citation

Matsuo, Miki, et al. "Isolation and Mutation Site Determination of the Temperature-sensitive murB Mutants of Staphylococcus Aureus." FEMS Microbiology Letters, vol. 222, no. 1, 2003, pp. 107-13.
Matsuo M, Kurokawa K, Nishida S, et al. Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus. FEMS Microbiol Lett. 2003;222(1):107-13.
Matsuo, M., Kurokawa, K., Nishida, S., Li, Y., Takimura, H., Kaito, C., Fukuhara, N., Maki, H., Miura, K., Murakami, K., & Sekimizu, K. (2003). Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus. FEMS Microbiology Letters, 222(1), 107-13.
Matsuo M, et al. Isolation and Mutation Site Determination of the Temperature-sensitive murB Mutants of Staphylococcus Aureus. FEMS Microbiol Lett. 2003 May 16;222(1):107-13. PubMed PMID: 12757953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus. AU - Matsuo,Miki, AU - Kurokawa,Kenji, AU - Nishida,Satoshi, AU - Li,Yan, AU - Takimura,Haruto, AU - Kaito,Chikara, AU - Fukuhara,Norio, AU - Maki,Hideki, AU - Miura,Kenji, AU - Murakami,Kazuhisa, AU - Sekimizu,Kazuhisa, PY - 2003/5/22/pubmed PY - 2003/8/7/medline PY - 2003/5/22/entrez SP - 107 EP - 13 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 222 IS - 1 N2 - The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the murB gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/12757953/Isolation_and_mutation_site_determination_of_the_temperature_sensitive_murB_mutants_of_Staphylococcus_aureus_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/S0378-1097(03)00260-X DB - PRIME DP - Unbound Medicine ER -