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Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome.
Can J Vet Res. 2003 May; 67(2):114-20.CJ

Abstract

A comparative serologic and virologic study was performed in pigs from 5 herds with postweaning multisystemic wasting syndrome (PMWS) and 2 herds without PMWS in Quebec. In each herd, 60 blood samples were collected at 4-wk intervals from pigs from 3 to 23 wk of age. The serum was evaluated for the presence of antibodies to porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as for the presence of nucleic acid of PCV2, PRRSV, an porcine parvovirus (PPV), by means of the polymerase chain reaction (PCR). Serologic profiles for PCV2 were very similar in 6 of the 7 herds, including the 2 without PMWS, and were characterized by a gradual decrease in antibody titres from 3 until 11 wk of age, followed by seroconversion at 15 wk, and high PCV2 antibody titres thereafter in all pigs. Only starting at 11 to 15 wk of age could PCV2 viremia be detected, except in 1 herd, in which clinical signs were observed at 6 to 7 wk of age. A PCV2 viremia could be detected within the same pigs for a minimum of 8 wk, and the virus could still be detected in 41% of the serum samples obtained at 23 wk of age. The antibody level did not appear to influence the occurrence of disease, since titres were similar in pigs in the herds with or without PMWS. Infection with PRRSV, as demonstrated by PCR and seroconversion, preceded that of PCV2 by at least 1 mo in both types of herd. Both PRRSV and PCV2 were detected in some pigs in 5 of the 7 herds, including 1 herd without PMWS. Porcine parvovirus could be detected in serum by PCR in 2 herds with PMWS after the onset of clinical signs and also in 1 herd without PMWS. Genomic analysis of PCV2 strains identified in the herds without PMWS indicated complete or very high homology (99.4% to 100%) with the PCV2 strains identified in 4 herds with PMWS. In our field study, the triggering of PMWS in the herds could not be linked to coinfection with either PRRSV or PPV or to the use of a specific immunostimulant, such as vaccines, or to particular genomic differences between the PCV2 strains identified.

Authors+Show Affiliations

Laboratoire d'hygiene vétérinaire et alimentaire, Agence canadienne d'inspection des aliments, 3400 Casavant ouest, St-Hyacinthe, Québec J2S 8E3. larocheller@inspection.gc.caNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12760476

Citation

Larochelle, Renée, et al. "Comparative Serologic and Virologic Study of Commercial Swine Herds With and Without Postweaning Multisystemic Wasting Syndrome." Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire, vol. 67, no. 2, 2003, pp. 114-20.
Larochelle R, Magar R, D'Allaire S. Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. Can J Vet Res. 2003;67(2):114-20.
Larochelle, R., Magar, R., & D'Allaire, S. (2003). Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire, 67(2), 114-20.
Larochelle R, Magar R, D'Allaire S. Comparative Serologic and Virologic Study of Commercial Swine Herds With and Without Postweaning Multisystemic Wasting Syndrome. Can J Vet Res. 2003;67(2):114-20. PubMed PMID: 12760476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. AU - Larochelle,Renée, AU - Magar,Ronald, AU - D'Allaire,Sylvie, PY - 2003/5/23/pubmed PY - 2003/8/13/medline PY - 2003/5/23/entrez SP - 114 EP - 20 JF - Canadian journal of veterinary research = Revue canadienne de recherche veterinaire JO - Can. J. Vet. Res. VL - 67 IS - 2 N2 - A comparative serologic and virologic study was performed in pigs from 5 herds with postweaning multisystemic wasting syndrome (PMWS) and 2 herds without PMWS in Quebec. In each herd, 60 blood samples were collected at 4-wk intervals from pigs from 3 to 23 wk of age. The serum was evaluated for the presence of antibodies to porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as for the presence of nucleic acid of PCV2, PRRSV, an porcine parvovirus (PPV), by means of the polymerase chain reaction (PCR). Serologic profiles for PCV2 were very similar in 6 of the 7 herds, including the 2 without PMWS, and were characterized by a gradual decrease in antibody titres from 3 until 11 wk of age, followed by seroconversion at 15 wk, and high PCV2 antibody titres thereafter in all pigs. Only starting at 11 to 15 wk of age could PCV2 viremia be detected, except in 1 herd, in which clinical signs were observed at 6 to 7 wk of age. A PCV2 viremia could be detected within the same pigs for a minimum of 8 wk, and the virus could still be detected in 41% of the serum samples obtained at 23 wk of age. The antibody level did not appear to influence the occurrence of disease, since titres were similar in pigs in the herds with or without PMWS. Infection with PRRSV, as demonstrated by PCR and seroconversion, preceded that of PCV2 by at least 1 mo in both types of herd. Both PRRSV and PCV2 were detected in some pigs in 5 of the 7 herds, including 1 herd without PMWS. Porcine parvovirus could be detected in serum by PCR in 2 herds with PMWS after the onset of clinical signs and also in 1 herd without PMWS. Genomic analysis of PCV2 strains identified in the herds without PMWS indicated complete or very high homology (99.4% to 100%) with the PCV2 strains identified in 4 herds with PMWS. In our field study, the triggering of PMWS in the herds could not be linked to coinfection with either PRRSV or PPV or to the use of a specific immunostimulant, such as vaccines, or to particular genomic differences between the PCV2 strains identified. SN - 0830-9000 UR - https://www.unboundmedicine.com/medline/citation/12760476/Comparative_serologic_and_virologic_study_of_commercial_swine_herds_with_and_without_postweaning_multisystemic_wasting_syndrome_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/12760476/ DB - PRIME DP - Unbound Medicine ER -