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Latency pattern of Epstein-Barr virus and methylation status in Epstein-Barr virus-associated hemophagocytic syndrome.
J Med Virol. 2003 Jul; 70(3):410-9.JM

Abstract

Expression of different panels of latent gene transcripts is controlled by usage of three distinct Epstein-Barr virus (EBV) nuclear antigen (EBNA) promoters (Wp, Cp, and Qp). EBV-associated hemophagocytic syndrome, which is often a fatal disease and generally occurs after primary EBV infection, is characterized by monoclonal or oligoclonal proliferation of EBV-infected T cells. The latency pattern and EBNA promoter (Wp, Cp, and Qp) usage in EBV-infected cells from three patients with EBV-associated hemophagocytic syndrome were examined by reverse transcription-polymerase chain reaction (PCR). Three samples from the patients expressed EBER, EBNA1, EBNA2, latent membrane protein (LMP)1, and LMP2A transcripts. The transcripts of EBNA1 were initiated from not only Wp/Cp but also Qp. Lytic cycle Fp-initiated EBNA1 and EBV lytic gene BZLF1 transcripts were not detected. The methylation statuses of three EBNA promoters in three patients with EBV-associated hemophagocytic syndrome and in two patients with infectious mononucleosis were also analyzed using bisulfite PCR analysis. Wp was hypermethylated, and Qp was unmethylated in both diseases. Cp was highly methylated in EBV-associated hemophagocytic syndrome, however, whereas Cp was almost unmethylated in infectious mononucleosis. These results suggest that there may be distinct EBV-infected cell populations in EBV-associated hemophagocytic syndrome, which exhibit different patterns of EBV latent gene expression. The methylation status in Cp and phenotype of EBV-infected cells may be critical differences in EBV-associated hemophagocytic syndrome and infectious mononucleosis.

Authors+Show Affiliations

Department of Pediatrics, Hokkaido University School of Medicine, Sapporo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12767005

Citation

Yoshioka, Mikio, et al. "Latency Pattern of Epstein-Barr Virus and Methylation Status in Epstein-Barr Virus-associated Hemophagocytic Syndrome." Journal of Medical Virology, vol. 70, no. 3, 2003, pp. 410-9.
Yoshioka M, Kikuta H, Ishiguro N, et al. Latency pattern of Epstein-Barr virus and methylation status in Epstein-Barr virus-associated hemophagocytic syndrome. J Med Virol. 2003;70(3):410-9.
Yoshioka, M., Kikuta, H., Ishiguro, N., Endo, R., & Kobayashi, K. (2003). Latency pattern of Epstein-Barr virus and methylation status in Epstein-Barr virus-associated hemophagocytic syndrome. Journal of Medical Virology, 70(3), 410-9.
Yoshioka M, et al. Latency Pattern of Epstein-Barr Virus and Methylation Status in Epstein-Barr Virus-associated Hemophagocytic Syndrome. J Med Virol. 2003;70(3):410-9. PubMed PMID: 12767005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Latency pattern of Epstein-Barr virus and methylation status in Epstein-Barr virus-associated hemophagocytic syndrome. AU - Yoshioka,Mikio, AU - Kikuta,Hideaki, AU - Ishiguro,Nobuhisa, AU - Endo,Rika, AU - Kobayashi,Kunihiko, PY - 2003/5/27/pubmed PY - 2003/8/2/medline PY - 2003/5/27/entrez SP - 410 EP - 9 JF - Journal of medical virology JO - J. Med. Virol. VL - 70 IS - 3 N2 - Expression of different panels of latent gene transcripts is controlled by usage of three distinct Epstein-Barr virus (EBV) nuclear antigen (EBNA) promoters (Wp, Cp, and Qp). EBV-associated hemophagocytic syndrome, which is often a fatal disease and generally occurs after primary EBV infection, is characterized by monoclonal or oligoclonal proliferation of EBV-infected T cells. The latency pattern and EBNA promoter (Wp, Cp, and Qp) usage in EBV-infected cells from three patients with EBV-associated hemophagocytic syndrome were examined by reverse transcription-polymerase chain reaction (PCR). Three samples from the patients expressed EBER, EBNA1, EBNA2, latent membrane protein (LMP)1, and LMP2A transcripts. The transcripts of EBNA1 were initiated from not only Wp/Cp but also Qp. Lytic cycle Fp-initiated EBNA1 and EBV lytic gene BZLF1 transcripts were not detected. The methylation statuses of three EBNA promoters in three patients with EBV-associated hemophagocytic syndrome and in two patients with infectious mononucleosis were also analyzed using bisulfite PCR analysis. Wp was hypermethylated, and Qp was unmethylated in both diseases. Cp was highly methylated in EBV-associated hemophagocytic syndrome, however, whereas Cp was almost unmethylated in infectious mononucleosis. These results suggest that there may be distinct EBV-infected cell populations in EBV-associated hemophagocytic syndrome, which exhibit different patterns of EBV latent gene expression. The methylation status in Cp and phenotype of EBV-infected cells may be critical differences in EBV-associated hemophagocytic syndrome and infectious mononucleosis. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12767005/Latency_pattern_of_Epstein_Barr_virus_and_methylation_status_in_Epstein_Barr_virus_associated_hemophagocytic_syndrome_ L2 - https://doi.org/10.1002/jmv.10411 DB - PRIME DP - Unbound Medicine ER -