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Influence of fish oil supplementation on in vivo and in vitro oxidation resistance of low-density lipoprotein in type 2 diabetes.
Eur J Clin Nutr. 2003 May; 57(5):713-20.EJ

Abstract

OBJECTIVE

Fish oil supplement has been proposed as a non-pharmacological strategy to correct the atherogenic lipid profile associated with type 2 diabetes mellitus. However, fish oil may have deleterious effects on lipid peroxidation and glycemic control.

DESIGN

In this study, 44 type 2 diabetic patients were randomized to vitamin E standardized (53.6 mg/day) supplementation (capsules) with 4 g daily of either fish oil (n=23) or corn oil (n=21) for 8 weeks preceded by a 4 week run-in period of corn oil supplementation. LDL was isolated by density gradient ultracentrifugation and oxidized in vitro with Cu(2+). As a marker of in vivo oxidation malondialdehyde concentration in LDL (LDL-MDA) was measured.

RESULTS

Fish oil reduced both mean lag time (before, 57.8; after, 48.8 min, P<0.001) and mean propagation rate (before, 0.018 DeltaOD/min; after, 0.015 DeltaOD/min, P<0.001), whereas corn oil had no influence on lag time and propagation rate. The changes in lag time and propagation rate differed significantly between fish oil and corn oil treatment. LDL-MDA changes differed borderline significantly between groups (FO, 110.4 pmol/mg protein; CO, 6.7 pmol/mg protein; P=0.057). Fish oil supplementation had no influence on glycemic control as assessed from HbA(1c) and fasting blood glucose.

CONCLUSION

According to our findings, fish oil supplementation leads to increased in vivo oxidation and increased in vitro oxidation susceptibility of LDL particles. More studies are needed to clarify the clinical importance of this finding.

Authors+Show Affiliations

Research Department of Human Nutrition, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12771973

Citation

Pedersen, H, et al. "Influence of Fish Oil Supplementation On in Vivo and in Vitro Oxidation Resistance of Low-density Lipoprotein in Type 2 Diabetes." European Journal of Clinical Nutrition, vol. 57, no. 5, 2003, pp. 713-20.
Pedersen H, Petersen M, Major-Pedersen A, et al. Influence of fish oil supplementation on in vivo and in vitro oxidation resistance of low-density lipoprotein in type 2 diabetes. Eur J Clin Nutr. 2003;57(5):713-20.
Pedersen, H., Petersen, M., Major-Pedersen, A., Jensen, T., Nielsen, N. S., Lauridsen, S. T., & Marckmann, P. (2003). Influence of fish oil supplementation on in vivo and in vitro oxidation resistance of low-density lipoprotein in type 2 diabetes. European Journal of Clinical Nutrition, 57(5), 713-20.
Pedersen H, et al. Influence of Fish Oil Supplementation On in Vivo and in Vitro Oxidation Resistance of Low-density Lipoprotein in Type 2 Diabetes. Eur J Clin Nutr. 2003;57(5):713-20. PubMed PMID: 12771973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of fish oil supplementation on in vivo and in vitro oxidation resistance of low-density lipoprotein in type 2 diabetes. AU - Pedersen,H, AU - Petersen,M, AU - Major-Pedersen,A, AU - Jensen,T, AU - Nielsen,N S, AU - Lauridsen,S T, AU - Marckmann,P, PY - 2002/01/18/received PY - 2002/07/22/revised PY - 2002/07/24/accepted PY - 2003/5/29/pubmed PY - 2003/9/4/medline PY - 2003/5/29/entrez SP - 713 EP - 20 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 57 IS - 5 N2 - OBJECTIVE: Fish oil supplement has been proposed as a non-pharmacological strategy to correct the atherogenic lipid profile associated with type 2 diabetes mellitus. However, fish oil may have deleterious effects on lipid peroxidation and glycemic control. DESIGN: In this study, 44 type 2 diabetic patients were randomized to vitamin E standardized (53.6 mg/day) supplementation (capsules) with 4 g daily of either fish oil (n=23) or corn oil (n=21) for 8 weeks preceded by a 4 week run-in period of corn oil supplementation. LDL was isolated by density gradient ultracentrifugation and oxidized in vitro with Cu(2+). As a marker of in vivo oxidation malondialdehyde concentration in LDL (LDL-MDA) was measured. RESULTS: Fish oil reduced both mean lag time (before, 57.8; after, 48.8 min, P<0.001) and mean propagation rate (before, 0.018 DeltaOD/min; after, 0.015 DeltaOD/min, P<0.001), whereas corn oil had no influence on lag time and propagation rate. The changes in lag time and propagation rate differed significantly between fish oil and corn oil treatment. LDL-MDA changes differed borderline significantly between groups (FO, 110.4 pmol/mg protein; CO, 6.7 pmol/mg protein; P=0.057). Fish oil supplementation had no influence on glycemic control as assessed from HbA(1c) and fasting blood glucose. CONCLUSION: According to our findings, fish oil supplementation leads to increased in vivo oxidation and increased in vitro oxidation susceptibility of LDL particles. More studies are needed to clarify the clinical importance of this finding. SN - 0954-3007 UR - https://www.unboundmedicine.com/medline/citation/12771973/Influence_of_fish_oil_supplementation_on_in_vivo_and_in_vitro_oxidation_resistance_of_low_density_lipoprotein_in_type_2_diabetes_ L2 - https://doi.org/10.1038/sj.ejcn.1601602 DB - PRIME DP - Unbound Medicine ER -