[Determination of curculigoside in crude medicine Curculigo orchioides by HPLC].Zhongguo Zhong Yao Za Zhi. 2002 Mar; 27(3):192-4.ZZ
To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.
The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm.
The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.
This method could be used to control the quality of crude medicine C. orchioides.