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[Determination of curculigoside in crude medicine Curculigo orchioides by HPLC].
Zhongguo Zhong Yao Za Zhi. 2002 Mar; 27(3):192-4.ZZ

Abstract

OBJECTIVE

To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.

METHOD

The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm.

RESULT

The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.

CONCLUSION

This method could be used to control the quality of crude medicine C. orchioides.

Authors+Show Affiliations

Guangdong Provincial Institute for Drug Control, Guangzhou 510180, Guangdong, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12774398

Citation

Lu, Hui-wen, et al. "[Determination of Curculigoside in Crude Medicine Curculigo Orchioides By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 27, no. 3, 2002, pp. 192-4.
Lu HW, Zhu BH, Liang YK. [Determination of curculigoside in crude medicine Curculigo orchioides by HPLC]. Zhongguo Zhong Yao Za Zhi. 2002;27(3):192-4.
Lu, H. W., Zhu, B. H., & Liang, Y. K. (2002). [Determination of curculigoside in crude medicine Curculigo orchioides by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 27(3), 192-4.
Lu HW, Zhu BH, Liang YK. [Determination of Curculigoside in Crude Medicine Curculigo Orchioides By HPLC]. Zhongguo Zhong Yao Za Zhi. 2002;27(3):192-4. PubMed PMID: 12774398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of curculigoside in crude medicine Curculigo orchioides by HPLC]. AU - Lu,Hui-wen, AU - Zhu,Bing-hui, AU - Liang,Ying-kang, PY - 2003/5/31/pubmed PY - 2003/9/25/medline PY - 2003/5/31/entrez SP - 192 EP - 4 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 27 IS - 3 N2 - OBJECTIVE: To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine. METHOD: The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm. RESULT: The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%. CONCLUSION: This method could be used to control the quality of crude medicine C. orchioides. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/12774398/[Determination_of_curculigoside_in_crude_medicine_Curculigo_orchioides_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -