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High-density lipoprotein stimulates endothelial cell migration and survival through sphingosine 1-phosphate and its receptors.
Arterioscler Thromb Vasc Biol. 2003 Jul 01; 23(7):1283-8.AT

Abstract

OBJECTIVE

Plasma high-density lipoprotein (HDL) level is inversely correlated with the risk of atherosclerosis. However, the cellular mechanism by which HDL exerts antiatherogenic actions is not well understood. In this study, we focus on the lipid components of HDL as mediators of the lipoprotein-induced antiatherogenic actions.

METHODS AND RESULTS

HDL and sphingosine 1-phosphate (S1P) stimulated the migration and survival of human umbilical vein endothelial cells. These responses to HDL and S1P were almost completely inhibited by pertussis toxin and other specific inhibitors for intracellular signaling pathways, although the inhibition profiles of migration and survival were different. The HDL-stimulated migration and survival of the cells were markedly inhibited by antisense oligonucleotides against the S1P receptors EDG-1/S1P1 and EDG-3/S1P3. Cell migration was sensitive to both receptors, but cell survival was exclusively sensitive to S1P1. The S1P-rich fraction and chromatographically purified S1P from HDL stimulated cell migration, but the rest of the fraction did not, as was the case of the cell survival.

CONCLUSIONS

HDL-induced endothelial cell migration and survival may be mediated by the lipoprotein component S1P and the lipid receptors S1P1 and S1P3.

Authors+Show Affiliations

Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12775579

Citation

Kimura, Takao, et al. "High-density Lipoprotein Stimulates Endothelial Cell Migration and Survival Through Sphingosine 1-phosphate and Its Receptors." Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 23, no. 7, 2003, pp. 1283-8.
Kimura T, Sato K, Malchinkhuu E, et al. High-density lipoprotein stimulates endothelial cell migration and survival through sphingosine 1-phosphate and its receptors. Arterioscler Thromb Vasc Biol. 2003;23(7):1283-8.
Kimura, T., Sato, K., Malchinkhuu, E., Tomura, H., Tamama, K., Kuwabara, A., Murakami, M., & Okajima, F. (2003). High-density lipoprotein stimulates endothelial cell migration and survival through sphingosine 1-phosphate and its receptors. Arteriosclerosis, Thrombosis, and Vascular Biology, 23(7), 1283-8.
Kimura T, et al. High-density Lipoprotein Stimulates Endothelial Cell Migration and Survival Through Sphingosine 1-phosphate and Its Receptors. Arterioscler Thromb Vasc Biol. 2003 Jul 1;23(7):1283-8. PubMed PMID: 12775579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-density lipoprotein stimulates endothelial cell migration and survival through sphingosine 1-phosphate and its receptors. AU - Kimura,Takao, AU - Sato,Koichi, AU - Malchinkhuu,Enkhzol, AU - Tomura,Hideaki, AU - Tamama,Kenichi, AU - Kuwabara,Atsushi, AU - Murakami,Masami, AU - Okajima,Fumikazu, Y1 - 2003/05/29/ PY - 2003/5/31/pubmed PY - 2004/2/6/medline PY - 2003/5/31/entrez SP - 1283 EP - 8 JF - Arteriosclerosis, thrombosis, and vascular biology JO - Arterioscler Thromb Vasc Biol VL - 23 IS - 7 N2 - OBJECTIVE: Plasma high-density lipoprotein (HDL) level is inversely correlated with the risk of atherosclerosis. However, the cellular mechanism by which HDL exerts antiatherogenic actions is not well understood. In this study, we focus on the lipid components of HDL as mediators of the lipoprotein-induced antiatherogenic actions. METHODS AND RESULTS: HDL and sphingosine 1-phosphate (S1P) stimulated the migration and survival of human umbilical vein endothelial cells. These responses to HDL and S1P were almost completely inhibited by pertussis toxin and other specific inhibitors for intracellular signaling pathways, although the inhibition profiles of migration and survival were different. The HDL-stimulated migration and survival of the cells were markedly inhibited by antisense oligonucleotides against the S1P receptors EDG-1/S1P1 and EDG-3/S1P3. Cell migration was sensitive to both receptors, but cell survival was exclusively sensitive to S1P1. The S1P-rich fraction and chromatographically purified S1P from HDL stimulated cell migration, but the rest of the fraction did not, as was the case of the cell survival. CONCLUSIONS: HDL-induced endothelial cell migration and survival may be mediated by the lipoprotein component S1P and the lipid receptors S1P1 and S1P3. SN - 1524-4636 UR - https://www.unboundmedicine.com/medline/citation/12775579/High_density_lipoprotein_stimulates_endothelial_cell_migration_and_survival_through_sphingosine_1_phosphate_and_its_receptors_ L2 - https://www.ahajournals.org/doi/10.1161/01.ATV.0000079011.67194.5A?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -