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Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations.
Thorax. 2003 Jun; 58(6):505-9.T

Abstract

BACKGROUND

Cysteinyl leukotrienes (Cys-LTs) and isoprostanes are inflammatory metabolites derived from arachidonic acid whose levels are increased in the airways of asthmatic patients. Isoprostanes are relatively stable and specific for lipid peroxidation, which makes them potentially reliable biomarkers for oxidative stress. A study was undertaken to evaluate the effect of a course of oral steroids on Cys-LT and 8-isoprostane levels in exhaled breath condensate of children with an asthma exacerbation.

METHODS

Exhaled breath condensate was collected and fractional exhaled nitric oxide (FE(NO)) and spirometric parameters were measured before and after a 5 day course of oral prednisone (1 mg/kg/day) in 15 asthmatic children with an asthma exacerbation. Cys-LT and 8-isoprostane concentrations were measured using an enzyme immunoassay. FE(NO) was measured using a chemiluminescence analyser. Exhaled breath condensate was also collected from 10 healthy children.

RESULTS

Before prednisone treatment both Cys-LT and 8-isoprostane concentrations were higher in asthmatic subjects (Cys-LTs, 12.7 pg/ml (IQR 5.4-15.6); 8-isoprostane, 12.0 pg/ml (9.4-29.5)) than in healthy children (Cys-LTs, 4.3 pg/ml (2.0-5.7), p=0.002; 8-isoprostane, 2.6 pg/ml (2.1-3.0), p<0.001). After prednisone treatment there was a significant decrease in both Cys-LT (5.2 pg/ml (3.9-8.8), p=0.005) and 8-isoprostane (8.4 pg/ml (5.4-11.6), p=0.04) concentrations, but 8-isoprostane levels remained higher than in controls (p<0.001). FE(NO) levels, which fell significantly after prednisone treatment (p<0.001), did not correlate significantly with either Cys-LT or 8-isoprostane concentrations.

CONCLUSION

After a 5 day course of oral prednisone there is a reduction in Cys-LT and 8-isoprostane levels in EBC of children with an asthma exacerbation, although 8-isoprostane levels remain higher than in controls. This finding suggests that corticosteroids may not be fully effective in reducing oxidative stress in children with an exacerbation of asthma.

Authors+Show Affiliations

Department of Pediatrics, University of Padova, Italy. eugi@pediatria.unipd.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12775861

Citation

Baraldi, E, et al. "Cysteinyl Leukotrienes and 8-isoprostane in Exhaled Breath Condensate of Children With Asthma Exacerbations." Thorax, vol. 58, no. 6, 2003, pp. 505-9.
Baraldi E, Carraro S, Alinovi R, et al. Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations. Thorax. 2003;58(6):505-9.
Baraldi, E., Carraro, S., Alinovi, R., Pesci, A., Ghiro, L., Bodini, A., Piacentini, G., Zacchello, F., & Zanconato, S. (2003). Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations. Thorax, 58(6), 505-9.
Baraldi E, et al. Cysteinyl Leukotrienes and 8-isoprostane in Exhaled Breath Condensate of Children With Asthma Exacerbations. Thorax. 2003;58(6):505-9. PubMed PMID: 12775861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations. AU - Baraldi,E, AU - Carraro,S, AU - Alinovi,R, AU - Pesci,A, AU - Ghiro,L, AU - Bodini,A, AU - Piacentini,G, AU - Zacchello,F, AU - Zanconato,S, PY - 2003/5/31/pubmed PY - 2003/7/8/medline PY - 2003/5/31/entrez SP - 505 EP - 9 JF - Thorax JO - Thorax VL - 58 IS - 6 N2 - BACKGROUND: Cysteinyl leukotrienes (Cys-LTs) and isoprostanes are inflammatory metabolites derived from arachidonic acid whose levels are increased in the airways of asthmatic patients. Isoprostanes are relatively stable and specific for lipid peroxidation, which makes them potentially reliable biomarkers for oxidative stress. A study was undertaken to evaluate the effect of a course of oral steroids on Cys-LT and 8-isoprostane levels in exhaled breath condensate of children with an asthma exacerbation. METHODS: Exhaled breath condensate was collected and fractional exhaled nitric oxide (FE(NO)) and spirometric parameters were measured before and after a 5 day course of oral prednisone (1 mg/kg/day) in 15 asthmatic children with an asthma exacerbation. Cys-LT and 8-isoprostane concentrations were measured using an enzyme immunoassay. FE(NO) was measured using a chemiluminescence analyser. Exhaled breath condensate was also collected from 10 healthy children. RESULTS: Before prednisone treatment both Cys-LT and 8-isoprostane concentrations were higher in asthmatic subjects (Cys-LTs, 12.7 pg/ml (IQR 5.4-15.6); 8-isoprostane, 12.0 pg/ml (9.4-29.5)) than in healthy children (Cys-LTs, 4.3 pg/ml (2.0-5.7), p=0.002; 8-isoprostane, 2.6 pg/ml (2.1-3.0), p<0.001). After prednisone treatment there was a significant decrease in both Cys-LT (5.2 pg/ml (3.9-8.8), p=0.005) and 8-isoprostane (8.4 pg/ml (5.4-11.6), p=0.04) concentrations, but 8-isoprostane levels remained higher than in controls (p<0.001). FE(NO) levels, which fell significantly after prednisone treatment (p<0.001), did not correlate significantly with either Cys-LT or 8-isoprostane concentrations. CONCLUSION: After a 5 day course of oral prednisone there is a reduction in Cys-LT and 8-isoprostane levels in EBC of children with an asthma exacerbation, although 8-isoprostane levels remain higher than in controls. This finding suggests that corticosteroids may not be fully effective in reducing oxidative stress in children with an exacerbation of asthma. SN - 0040-6376 UR - https://www.unboundmedicine.com/medline/citation/12775861/Cysteinyl_leukotrienes_and_8_isoprostane_in_exhaled_breath_condensate_of_children_with_asthma_exacerbations_ L2 - https://thorax.bmj.com/lookup/pmidlookup?view=long&amp;pmid=12775861 DB - PRIME DP - Unbound Medicine ER -