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[Determination of ursolic acid in herba of Verbena officinalis by HPLC].
Zhongguo Zhong Yao Za Zhi. 2002 Dec; 27(12):916-8.ZZ

Abstract

OBJECTIVE

To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis.

METHOD

The column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm.

RESULTS

The liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%.

CONCLUSION

The method is simple, accurate and suitable for the qualify control.

Authors+Show Affiliations

Liu CH
Institute for Drug and Instrument Control of PLA, Beijing 100071, China. liuchenghong@chinaren.net
Liu Y
No affiliation info available

MeSH

Chromatography, High Pressure LiquidPlant Components, AerialPlants, MedicinalQuality ControlTriterpenesVerbena

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12776531

Citation

Liu, Cheng-hong, and Yun Liu. "[Determination of Ursolic Acid in Herba of Verbena Officinalis By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 27, no. 12, 2002, pp. 916-8.
Liu CH, Liu Y. [Determination of ursolic acid in herba of Verbena officinalis by HPLC]. Zhongguo Zhong Yao Za Zhi. 2002;27(12):916-8.
Liu, C. H., & Liu, Y. (2002). [Determination of ursolic acid in herba of Verbena officinalis by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 27(12), 916-8.
Liu CH, Liu Y. [Determination of Ursolic Acid in Herba of Verbena Officinalis By HPLC]. Zhongguo Zhong Yao Za Zhi. 2002;27(12):916-8. PubMed PMID: 12776531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of ursolic acid in herba of Verbena officinalis by HPLC]. AU - Liu,Cheng-hong, AU - Liu,Yun, PY - 2003/6/5/pubmed PY - 2003/10/24/medline PY - 2003/6/5/entrez SP - 916 EP - 8 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 27 IS - 12 N2 - OBJECTIVE: To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis. METHOD: The column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm. RESULTS: The liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%. CONCLUSION: The method is simple, accurate and suitable for the qualify control. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/12776531/[Determination_of_ursolic_acid_in_herba_of_Verbena_officinalis_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -