Tags

Type your tag names separated by a space and hit enter

[Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in mice].
Yao Xue Xue Bao. 2003 Feb; 38(2):138-42.YX

Abstract

AIM

To evaluate the hypoglycemic effect of chitosan-coated and sodium alginate-coated insulin liposomes after oral administration in mice.

METHODS

Insulin-liposomes were prepared by reverse-phase evaporation. Chitosan and alginate coating was carried out by mixing liposomal suspension with chitosan and sodium alginate solutions, followed by incubation. The particle size and morphology of insulin-liposomes were determined using laser light scattering instrument and transmission electron microscopy (TEM). The entrapment efficiency was analyzed using HPLC and ultracentrifuge. The protection of insulin from peptic and tryptic digestion was studied with HPLC. The hypoglycemic effects of polysaccharide-coated insulin liposomes were investigated using the glucose oxidase method after oral administration in mice.

RESULTS

The particle size of uncoated, chitosan-coated and alginate-coated insulin-liposomes was (138 +/- 31) nm, (230 +/- 20) nm and (266 +/- 19) nm, respectively. All insulin-liposomes were of spherical or ellipsoidal shape. The entrapment efficiencies were 81.6%, 73.5% and 68.7%, respectively. Insulin was protected from tryptic digestion by chitosan-coated liposomes and protected from peptic digestion by alginate-coated liposomes. The hypoglycemic effects of insulin-liposomes, coated with 0.1% chitosan and 0.1% sodium alginate, were observed.

CONCLUSION

Chitosan-coated and sodium alginate-coated liposomes were shown to reduce peptic or tryptic digestion on insulin, and enhance enteral absorption of insulin.

Authors+Show Affiliations

Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12778751

Citation

Wu, Zheng-hong, et al. "[Hypoglycemic Effect of Polysaccharide-coated Insulin Liposomes After Oral Administration in Mice]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 38, no. 2, 2003, pp. 138-42.
Wu ZH, Ping QN, Lai JM, et al. [Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in mice]. Yao Xue Xue Bao. 2003;38(2):138-42.
Wu, Z. H., Ping, Q. N., Lai, J. M., & Wei, Y. (2003). [Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in mice]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 38(2), 138-42.
Wu ZH, et al. [Hypoglycemic Effect of Polysaccharide-coated Insulin Liposomes After Oral Administration in Mice]. Yao Xue Xue Bao. 2003;38(2):138-42. PubMed PMID: 12778751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in mice]. AU - Wu,Zheng-hong, AU - Ping,Qi-neng, AU - Lai,Jia-ming, AU - Wei,Yi, PY - 2003/6/5/pubmed PY - 2004/4/20/medline PY - 2003/6/5/entrez SP - 138 EP - 42 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 38 IS - 2 N2 - AIM: To evaluate the hypoglycemic effect of chitosan-coated and sodium alginate-coated insulin liposomes after oral administration in mice. METHODS: Insulin-liposomes were prepared by reverse-phase evaporation. Chitosan and alginate coating was carried out by mixing liposomal suspension with chitosan and sodium alginate solutions, followed by incubation. The particle size and morphology of insulin-liposomes were determined using laser light scattering instrument and transmission electron microscopy (TEM). The entrapment efficiency was analyzed using HPLC and ultracentrifuge. The protection of insulin from peptic and tryptic digestion was studied with HPLC. The hypoglycemic effects of polysaccharide-coated insulin liposomes were investigated using the glucose oxidase method after oral administration in mice. RESULTS: The particle size of uncoated, chitosan-coated and alginate-coated insulin-liposomes was (138 +/- 31) nm, (230 +/- 20) nm and (266 +/- 19) nm, respectively. All insulin-liposomes were of spherical or ellipsoidal shape. The entrapment efficiencies were 81.6%, 73.5% and 68.7%, respectively. Insulin was protected from tryptic digestion by chitosan-coated liposomes and protected from peptic digestion by alginate-coated liposomes. The hypoglycemic effects of insulin-liposomes, coated with 0.1% chitosan and 0.1% sodium alginate, were observed. CONCLUSION: Chitosan-coated and sodium alginate-coated liposomes were shown to reduce peptic or tryptic digestion on insulin, and enhance enteral absorption of insulin. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/12778751/[Hypoglycemic_effect_of_polysaccharide_coated_insulin_liposomes_after_oral_administration_in_mice]_ DB - PRIME DP - Unbound Medicine ER -