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A fat-enriched, glucose-enriched diet markedly attenuates adiponectin mRNA levels in rat epididymal adipose tissue.
Clin Sci (Lond) 2003; 105(4):403-8CS

Abstract

Adiponectin levels are decreased in subjects with obesity, diabetes and coronary artery disease. In the present study, we have investigated whether the decrease in the levels and mRNA expression of adiponectin is due to obesity or to the diet itself. Wistar rats were either fed standard laboratory chow throughout (controls) or given a fat-enriched, glucose-enriched diet (diet-fed) for 2 days or 16 weeks. After 2 days of diet feeding, total body weight, fat pad masses and the plasma levels of glucose, insulin and leptin were all comparable between the two groups, while plasma NEFA (non-esterified fatty acid) and triacylglycerol levels were increased in the diet-fed animals (P<0.01 for both). There was a marked (P<0.01) decrease in plasma adiponectin levels. After 16 weeks of diet feeding, diet-fed rats had significantly higher body weight, fat pad mass and plasma levels of leptin, adiponectin, NEFA and triacylglycerol (P<0.001 for all) compared with chow-fed controls, whereas plasma levels of glucose and insulin were similar in the two groups. After 2 days of diet feeding, there were no significant changes in Ob mRNA levels in epididymal fat, whereas there was a marked decrease in adiponectin mRNA levels. After 16 weeks of diet feeding, rats had significantly increased levels of Ob mRNA, but decreased adiponectin mRNA levels, in epididymal fat compared with the chow-fed group (P<0.001 for both). These findings suggest that obesity per se is not a factor in the decreased adiponectin levels observed in obese subjects. We propose that the lipid profile of the plasma and/or the constituents of the diet consumed by rats may contribute to adiponectin levels more than obesity per se.

Authors+Show Affiliations

Neuroendocrine and Obesity Biology Unit, Department of Medicine, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK. naderali@liverpool.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12780342

Citation

Naderali, Ebrahim K., et al. "A Fat-enriched, Glucose-enriched Diet Markedly Attenuates Adiponectin mRNA Levels in Rat Epididymal Adipose Tissue." Clinical Science (London, England : 1979), vol. 105, no. 4, 2003, pp. 403-8.
Naderali EK, Estadella D, Rocha M, et al. A fat-enriched, glucose-enriched diet markedly attenuates adiponectin mRNA levels in rat epididymal adipose tissue. Clin Sci. 2003;105(4):403-8.
Naderali, E. K., Estadella, D., Rocha, M., Pickavance, L. C., Fatani, S., Denis, R. G., & Williams, G. (2003). A fat-enriched, glucose-enriched diet markedly attenuates adiponectin mRNA levels in rat epididymal adipose tissue. Clinical Science (London, England : 1979), 105(4), pp. 403-8.
Naderali EK, et al. A Fat-enriched, Glucose-enriched Diet Markedly Attenuates Adiponectin mRNA Levels in Rat Epididymal Adipose Tissue. Clin Sci. 2003;105(4):403-8. PubMed PMID: 12780342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fat-enriched, glucose-enriched diet markedly attenuates adiponectin mRNA levels in rat epididymal adipose tissue. AU - Naderali,Ebrahim K, AU - Estadella,Debora, AU - Rocha,Milagros, AU - Pickavance,Lucy C, AU - Fatani,Sameer, AU - Denis,Raphael G P, AU - Williams,Gareth, PY - 2003/06/03/accepted PY - 2003/05/27/revised PY - 2003/03/12/received PY - 2003/6/5/pubmed PY - 2003/11/11/medline PY - 2003/6/5/entrez SP - 403 EP - 8 JF - Clinical science (London, England : 1979) JO - Clin. Sci. VL - 105 IS - 4 N2 - Adiponectin levels are decreased in subjects with obesity, diabetes and coronary artery disease. In the present study, we have investigated whether the decrease in the levels and mRNA expression of adiponectin is due to obesity or to the diet itself. Wistar rats were either fed standard laboratory chow throughout (controls) or given a fat-enriched, glucose-enriched diet (diet-fed) for 2 days or 16 weeks. After 2 days of diet feeding, total body weight, fat pad masses and the plasma levels of glucose, insulin and leptin were all comparable between the two groups, while plasma NEFA (non-esterified fatty acid) and triacylglycerol levels were increased in the diet-fed animals (P<0.01 for both). There was a marked (P<0.01) decrease in plasma adiponectin levels. After 16 weeks of diet feeding, diet-fed rats had significantly higher body weight, fat pad mass and plasma levels of leptin, adiponectin, NEFA and triacylglycerol (P<0.001 for all) compared with chow-fed controls, whereas plasma levels of glucose and insulin were similar in the two groups. After 2 days of diet feeding, there were no significant changes in Ob mRNA levels in epididymal fat, whereas there was a marked decrease in adiponectin mRNA levels. After 16 weeks of diet feeding, rats had significantly increased levels of Ob mRNA, but decreased adiponectin mRNA levels, in epididymal fat compared with the chow-fed group (P<0.001 for both). These findings suggest that obesity per se is not a factor in the decreased adiponectin levels observed in obese subjects. We propose that the lipid profile of the plasma and/or the constituents of the diet consumed by rats may contribute to adiponectin levels more than obesity per se. SN - 0143-5221 UR - https://www.unboundmedicine.com/medline/citation/12780342/A_fat_enriched_glucose_enriched_diet_markedly_attenuates_adiponectin_mRNA_levels_in_rat_epididymal_adipose_tissue_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/CS20030094 DB - PRIME DP - Unbound Medicine ER -