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cDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, Araneus ventricosus.
Comp Biochem Physiol B Biochem Mol Biol. 2003 May; 135(1):117-23.CB

Abstract

A cDNA encoding a defender against apoptotic cell death (DAD1) homologue was cloned from a cDNA library of the spider, A. ventricosus. Sequence analysis of the cDNA encoding the DAD1 homologue of A. ventricosus revealed that the 339-bp cDNA has an open reading frame of 113 amino acid residues. The deduced amino acid sequence of the A. ventricosus DAD1 homologue showed 75.4% of identity to Drosophila melanogaster, 74.6% of identity to Xenopus laevis, and 73.1% of identity to Homo sapiens, Sus scrofa, Mesocricetus auratus, Rattus norvegicus and Mus musculus. All animal DAD1 including A. ventricosus DAD1 homologue formed a subgroup, excluding all plant DAD1 proteins in the phylogenetic analysis. Northern blot analysis shows that the transcripts of A. ventricosus DAD1 homologue gene are present in all tissues examined, suggesting that A. ventricosus DAD1 is expressed in most, if not all, body tissues. Interestingly, the transcript levels of A. ventricosus DAD1 homologue gene were particularly high when exposed at low (4 degrees C) and high (37 degrees C) temperatures, suggesting that the gene is responsive to temperature stimuli.

Authors+Show Affiliations

Division of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12781979

Citation

Sik Lee, Kwang, et al. "CDNA Cloning of a Defender Against Apoptotic Cell Death 1 (DAD1) Homologue, Responsive to External Temperature Stimulus From the Spider, Araneus Ventricosus." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 135, no. 1, 2003, pp. 117-23.
Sik Lee K, Hwa Chung E, Hee Han J, et al. CDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, Araneus ventricosus. Comp Biochem Physiol B Biochem Mol Biol. 2003;135(1):117-23.
Sik Lee, K., Hwa Chung, E., Hee Han, J., Dae Sohn, H., & Rae Jin, B. (2003). CDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, Araneus ventricosus. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 135(1), 117-23.
Sik Lee K, et al. CDNA Cloning of a Defender Against Apoptotic Cell Death 1 (DAD1) Homologue, Responsive to External Temperature Stimulus From the Spider, Araneus Ventricosus. Comp Biochem Physiol B Biochem Mol Biol. 2003;135(1):117-23. PubMed PMID: 12781979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - cDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, Araneus ventricosus. AU - Sik Lee,Kwang, AU - Hwa Chung,Eun, AU - Hee Han,Ji, AU - Dae Sohn,Hung, AU - Rae Jin,Byung, PY - 2003/6/5/pubmed PY - 2004/3/3/medline PY - 2003/6/5/entrez SP - 117 EP - 23 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp Biochem Physiol B Biochem Mol Biol VL - 135 IS - 1 N2 - A cDNA encoding a defender against apoptotic cell death (DAD1) homologue was cloned from a cDNA library of the spider, A. ventricosus. Sequence analysis of the cDNA encoding the DAD1 homologue of A. ventricosus revealed that the 339-bp cDNA has an open reading frame of 113 amino acid residues. The deduced amino acid sequence of the A. ventricosus DAD1 homologue showed 75.4% of identity to Drosophila melanogaster, 74.6% of identity to Xenopus laevis, and 73.1% of identity to Homo sapiens, Sus scrofa, Mesocricetus auratus, Rattus norvegicus and Mus musculus. All animal DAD1 including A. ventricosus DAD1 homologue formed a subgroup, excluding all plant DAD1 proteins in the phylogenetic analysis. Northern blot analysis shows that the transcripts of A. ventricosus DAD1 homologue gene are present in all tissues examined, suggesting that A. ventricosus DAD1 is expressed in most, if not all, body tissues. Interestingly, the transcript levels of A. ventricosus DAD1 homologue gene were particularly high when exposed at low (4 degrees C) and high (37 degrees C) temperatures, suggesting that the gene is responsive to temperature stimuli. SN - 1096-4959 UR - https://www.unboundmedicine.com/medline/citation/12781979/cDNA_cloning_of_a_defender_against_apoptotic_cell_death_1__DAD1__homologue_responsive_to_external_temperature_stimulus_from_the_spider_Araneus_ventricosus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096495903000551 DB - PRIME DP - Unbound Medicine ER -