TY - JOUR T1 - Dendritic-cell-specific ICAM3-grabbing non-integrin is essential for the productive infection of human dendritic cells by mosquito-cell-derived dengue viruses. AU - Navarro-Sanchez,Erika, AU - Altmeyer,Ralf, AU - Amara,Ali, AU - Schwartz,Olivier, AU - Fieschi,Franck, AU - Virelizier,Jean-Louis, AU - Arenzana-Seisdedos,Fernando, AU - Desprès,Philippe, PY - 2003/02/06/received PY - 2003/04/07/revised PY - 2003/04/24/accepted PY - 2003/6/5/pubmed PY - 2004/4/21/medline PY - 2003/6/5/entrez SP - 723 EP - 8 JF - EMBO reports JO - EMBO Rep VL - 4 IS - 7 N2 - Dengue virus (DV) is a mosquito-borne flavivirus that causes haemorrhagic fever in humans. DV primarily targets immature dendritic cells (DCs) after a bite by an infected mosquito vector. Here, we analysed the interactions between DV and human-monocyte-derived DCs at the level of virus entry. We show that the DC-specific ICAM3-grabbing non-integrin (DC-SIGN) molecule, a cell-surface, mannose-specific, C-type lectin, binds mosquito-cell-derived DVs and allows viral replication. Conclusive evidence for the involvement of DC-SIGN in DV infection was obtained by the inhibition of viral infection by anti-DC-SIGN antibodies and by the soluble tetrameric ectodomain of DC-SIGN. Our data show that DC-SIGN functions as a DV-binding lectin by interacting with the DV envelope glycoprotein. Mosquito-cell-derived DVs may have differential infectivity for DC-SIGN-expressing cells. We suggest that the differential use of DC-SIGN by viral envelope glycoproteins may account for the immunopathogenesis of DVs. SN - 1469-221X UR - https://www.unboundmedicine.com/medline/citation/12783086/Dendritic_cell_specific_ICAM3_grabbing_non_integrin_is_essential_for_the_productive_infection_of_human_dendritic_cells_by_mosquito_cell_derived_dengue_viruses_ DB - PRIME DP - Unbound Medicine ER -