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Distinct roles of P2X receptors in modulating glutamate release at different primary sensory synapses in rat spinal cord.
J Neurophysiol. 2003 Jun; 89(6):3243-52.JN

Abstract

Using spinal cord slice preparations and patch-clamp recordings in lamina II and lamina V regions, we tested a hypothesis that P2X receptor subtypes differentially modulate glutamate release from primary afferent terminals innervating different sensory regions. We found that activation of P2X receptors by alpha,beta-methylene-ATP increased glutamate release onto >80% of DH neurons in both lamina regions. However, two distinct types of modulation, a transient and a long-lasting enhancement of glutamate release were observed. In lamina II recordings, >70% of the modulation was transient. In contrast, P2X receptor-mediated modulation was always long-lasting in lamina V. Pharmacologically, both transient and long-lasting types of modulation were blocked by 10 microM pyridxal-phosphate-6-azophenyl-2',4'-disulphonic acid tetrasodium, a broad-spectrum P2X receptor antagonist. Transient modulation was not observed in the presence of 1 microM trinitrophenyl-ATP (TNP-ATP), a subtype-selective P2X receptor antagonist, suggesting that homomeric P2X3 receptors may be involved in the transient modulation in lamina II. The long-lasting modulation remained in the presence of 1 microM TNP-ATP. Selective removal of P2X3-expressing afferent terminals by the targeting toxin saporin-conjugated isolectin B4 or surgical removal of superficial DH did not affect P2X receptor-mediated long-lasting modulation in lamina V. Taken together, these results suggest that P2X receptor subtypes play distinct roles in sensory processing in functionally different sensory regions.

Authors+Show Affiliations

McKnight Brain Institute and Department of Oral Surgery, Division of Neuroscience, College of Dentistry, University of Florida, Gainesville, Florida, 32610, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12783958

Citation

Nakatsuka, Terumasa, et al. "Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord." Journal of Neurophysiology, vol. 89, no. 6, 2003, pp. 3243-52.
Nakatsuka T, Tsuzuki K, Ling JX, et al. Distinct roles of P2X receptors in modulating glutamate release at different primary sensory synapses in rat spinal cord. J Neurophysiol. 2003;89(6):3243-52.
Nakatsuka, T., Tsuzuki, K., Ling, J. X., Sonobe, H., & Gu, J. G. (2003). Distinct roles of P2X receptors in modulating glutamate release at different primary sensory synapses in rat spinal cord. Journal of Neurophysiology, 89(6), 3243-52.
Nakatsuka T, et al. Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord. J Neurophysiol. 2003;89(6):3243-52. PubMed PMID: 12783958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct roles of P2X receptors in modulating glutamate release at different primary sensory synapses in rat spinal cord. AU - Nakatsuka,Terumasa, AU - Tsuzuki,Kenzo, AU - Ling,Jennifer X, AU - Sonobe,Hideki, AU - Gu,Jianguo G, PY - 2003/6/5/pubmed PY - 2003/8/2/medline PY - 2003/6/5/entrez SP - 3243 EP - 52 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 89 IS - 6 N2 - Using spinal cord slice preparations and patch-clamp recordings in lamina II and lamina V regions, we tested a hypothesis that P2X receptor subtypes differentially modulate glutamate release from primary afferent terminals innervating different sensory regions. We found that activation of P2X receptors by alpha,beta-methylene-ATP increased glutamate release onto >80% of DH neurons in both lamina regions. However, two distinct types of modulation, a transient and a long-lasting enhancement of glutamate release were observed. In lamina II recordings, >70% of the modulation was transient. In contrast, P2X receptor-mediated modulation was always long-lasting in lamina V. Pharmacologically, both transient and long-lasting types of modulation were blocked by 10 microM pyridxal-phosphate-6-azophenyl-2',4'-disulphonic acid tetrasodium, a broad-spectrum P2X receptor antagonist. Transient modulation was not observed in the presence of 1 microM trinitrophenyl-ATP (TNP-ATP), a subtype-selective P2X receptor antagonist, suggesting that homomeric P2X3 receptors may be involved in the transient modulation in lamina II. The long-lasting modulation remained in the presence of 1 microM TNP-ATP. Selective removal of P2X3-expressing afferent terminals by the targeting toxin saporin-conjugated isolectin B4 or surgical removal of superficial DH did not affect P2X receptor-mediated long-lasting modulation in lamina V. Taken together, these results suggest that P2X receptor subtypes play distinct roles in sensory processing in functionally different sensory regions. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/12783958/Distinct_roles_of_P2X_receptors_in_modulating_glutamate_release_at_different_primary_sensory_synapses_in_rat_spinal_cord_ L2 - http://www.physiology.org/doi/full/10.1152/jn.01172.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -