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Effects of high intensity resistance and low intensity endurance training on myosin heavy chain isoform expression in highly trained rowers.
Int J Sports Med. 2003 May; 24(4):264-70.IJ

Abstract

An important mechanism of muscle adaptation to exercise is the alteration of myosin heavy chain (MHC) isoform expression. This study investigated the effect of a high intensity resistance training (HIRT) and a low intensity endurance rowing (LIER) on MHC isoform expression in highly trained human muscle. Six well-trained male rowers underwent a training program consisting of a 3-week HIRT and a 3-week LIER, each followed by one-week of recovery. Muscle samples were taken from vastus lateralis before and at the end of each training and recovery phase. MHC isoform was analyzed by SDS-PAGE using silver stain and MHC isoform mRNA by RT-PCR. The maximum oxygen uptake and power output did not change after the training. MHC isoform composition did not change over HIRT or LIER, and there was a decrease in MHC I with concomitant increase in MHC IIa after recovery following HIRT. HIRT led to mRNA upregulation of MHC Ialpha, Ibeta and IIx (127 %, 148 % and 117 %, respectively, p < 0.05), but not MHC I protein (60 % vs 62 %, NS), and LIER led merely to MHC Ibeta mRNA upregulation (131 %, p < 0.05). Thus, different responses of MHC isoform expression to HIRT and LIER occurred in the highly trained muscle, and a "ceiling effect" in terms of MHC I expression could be observed. The upregulation of MHC Ialpha mRNA in human skeletal muscle documented in this study may encourage further observations in this field.

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Authors+Show Affiliations

Liu Y
Department of Sports and Rehabilitation Medicine, University of Ulm, Ulm, Germany. yuefei.liu@medizin.uni-ulm.de
Lormes W
No affiliation info available
Reissnecker S
No affiliation info available
Steinacker JM
No affiliation info available

MeSH

AdultBiopsy, NeedleHumansMaleMuscle, SkeletalMyosin Heavy ChainsOxygen ConsumptionPhysical Education and TrainingPhysical EnduranceProtein IsoformsRNA, MessengerSportsWeight Lifting

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12784168

Citation

Liu, Y, et al. "Effects of High Intensity Resistance and Low Intensity Endurance Training On Myosin Heavy Chain Isoform Expression in Highly Trained Rowers." International Journal of Sports Medicine, vol. 24, no. 4, 2003, pp. 264-70.
Liu Y, Lormes W, Reissnecker S, et al. Effects of high intensity resistance and low intensity endurance training on myosin heavy chain isoform expression in highly trained rowers. Int J Sports Med. 2003;24(4):264-70.
Liu, Y., Lormes, W., Reissnecker, S., & Steinacker, J. M. (2003). Effects of high intensity resistance and low intensity endurance training on myosin heavy chain isoform expression in highly trained rowers. International Journal of Sports Medicine, 24(4), 264-70.
Liu Y, et al. Effects of High Intensity Resistance and Low Intensity Endurance Training On Myosin Heavy Chain Isoform Expression in Highly Trained Rowers. Int J Sports Med. 2003;24(4):264-70. PubMed PMID: 12784168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of high intensity resistance and low intensity endurance training on myosin heavy chain isoform expression in highly trained rowers. AU - Liu,Y, AU - Lormes,W, AU - Reissnecker,S, AU - Steinacker,J M, PY - 2003/6/5/pubmed PY - 2003/10/3/medline PY - 2003/6/5/entrez SP - 264 EP - 70 JF - International journal of sports medicine JO - Int J Sports Med VL - 24 IS - 4 N2 - An important mechanism of muscle adaptation to exercise is the alteration of myosin heavy chain (MHC) isoform expression. This study investigated the effect of a high intensity resistance training (HIRT) and a low intensity endurance rowing (LIER) on MHC isoform expression in highly trained human muscle. Six well-trained male rowers underwent a training program consisting of a 3-week HIRT and a 3-week LIER, each followed by one-week of recovery. Muscle samples were taken from vastus lateralis before and at the end of each training and recovery phase. MHC isoform was analyzed by SDS-PAGE using silver stain and MHC isoform mRNA by RT-PCR. The maximum oxygen uptake and power output did not change after the training. MHC isoform composition did not change over HIRT or LIER, and there was a decrease in MHC I with concomitant increase in MHC IIa after recovery following HIRT. HIRT led to mRNA upregulation of MHC Ialpha, Ibeta and IIx (127 %, 148 % and 117 %, respectively, p < 0.05), but not MHC I protein (60 % vs 62 %, NS), and LIER led merely to MHC Ibeta mRNA upregulation (131 %, p < 0.05). Thus, different responses of MHC isoform expression to HIRT and LIER occurred in the highly trained muscle, and a "ceiling effect" in terms of MHC I expression could be observed. The upregulation of MHC Ialpha mRNA in human skeletal muscle documented in this study may encourage further observations in this field. SN - 0172-4622 UR - https://www.unboundmedicine.com/medline/citation/12784168/Effects_of_high_intensity_resistance_and_low_intensity_endurance_training_on_myosin_heavy_chain_isoform_expression_in_highly_trained_rowers_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2003-39509 DB - PRIME DP - Unbound Medicine ER -