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Enhancing the efficacy of chemotherapeutic drugs by the suppression of antiapoptotic cellular defense.
Cancer Detect Prev. 2003; 27(3):193-202.CD

Abstract

The study was aimed at evaluating the combination of a traditional anticancer drug doxorubicin (DOX) with a suppressor of antiapoptotic cellular defense--synthetic peptide corresponding to the minimal sequence of BCL-2 homology 3 (BH3) domain. BH3 peptide was delivered into cells by fusion with a peptide corresponding to the Antennapedia (Ant) internalization sequence. The cytotoxicity of DOX, Ant-BH3 and Ant-BH3 mixed in with DOX, mitochondrial transmembrane potential, expression of genes encoding pro- and antiapoptotic members of BCL-2 protein family and caspases, caspases activity, apoptosis induction were assessed in human ovarian carcinoma cells. It was found that the combination in one drug formulation of DOX and Ant-BH3 produced two main effects: (1) enhancing the apoptosis induction by an anticancer drug, and (2) preventing the development of antiapoptotic cellular drug resistance. The results confirmed that anticancer drug-BH3 combination might form the basis for a new advanced anticancer proapoptotic drug delivery systems.

Authors+Show Affiliations

Department of Pharmaceutics, Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA. minko@cop.rutgers.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12787726

Citation

Minko, T, et al. "Enhancing the Efficacy of Chemotherapeutic Drugs By the Suppression of Antiapoptotic Cellular Defense." Cancer Detection and Prevention, vol. 27, no. 3, 2003, pp. 193-202.
Minko T, Dharap SS, Fabbricatore AT. Enhancing the efficacy of chemotherapeutic drugs by the suppression of antiapoptotic cellular defense. Cancer Detect Prev. 2003;27(3):193-202.
Minko, T., Dharap, S. S., & Fabbricatore, A. T. (2003). Enhancing the efficacy of chemotherapeutic drugs by the suppression of antiapoptotic cellular defense. Cancer Detection and Prevention, 27(3), 193-202.
Minko T, Dharap SS, Fabbricatore AT. Enhancing the Efficacy of Chemotherapeutic Drugs By the Suppression of Antiapoptotic Cellular Defense. Cancer Detect Prev. 2003;27(3):193-202. PubMed PMID: 12787726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancing the efficacy of chemotherapeutic drugs by the suppression of antiapoptotic cellular defense. AU - Minko,T, AU - Dharap,S S, AU - Fabbricatore,A T, PY - 2003/6/6/pubmed PY - 2003/10/24/medline PY - 2003/6/6/entrez SP - 193 EP - 202 JF - Cancer detection and prevention JO - Cancer Detect Prev VL - 27 IS - 3 N2 - The study was aimed at evaluating the combination of a traditional anticancer drug doxorubicin (DOX) with a suppressor of antiapoptotic cellular defense--synthetic peptide corresponding to the minimal sequence of BCL-2 homology 3 (BH3) domain. BH3 peptide was delivered into cells by fusion with a peptide corresponding to the Antennapedia (Ant) internalization sequence. The cytotoxicity of DOX, Ant-BH3 and Ant-BH3 mixed in with DOX, mitochondrial transmembrane potential, expression of genes encoding pro- and antiapoptotic members of BCL-2 protein family and caspases, caspases activity, apoptosis induction were assessed in human ovarian carcinoma cells. It was found that the combination in one drug formulation of DOX and Ant-BH3 produced two main effects: (1) enhancing the apoptosis induction by an anticancer drug, and (2) preventing the development of antiapoptotic cellular drug resistance. The results confirmed that anticancer drug-BH3 combination might form the basis for a new advanced anticancer proapoptotic drug delivery systems. SN - 0361-090X UR - https://www.unboundmedicine.com/medline/citation/12787726/Enhancing_the_efficacy_of_chemotherapeutic_drugs_by_the_suppression_of_antiapoptotic_cellular_defense_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361090X03000679 DB - PRIME DP - Unbound Medicine ER -